نوع مقاله : مقاله پژوهشی

نویسندگان

1 نویسنده مسئول، گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه، ایران. رایانامه: zmostafai@basu.ac.ir

2 گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه، ایران. رایانامه: torki@razi.ac.ir

3 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه، ایران. رایانامه: cheghamirza@ razi.ac.ir

4 گروه حفظ نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه، ایران. رایانامه: r.sharifi@razi.ac.ir

10.22059/jap.2024.367708.623768

چکیده

اثر خوراندن مستقیم میکروارگانیسم بر عملکرد، میکرو فلور روده باریک و کور، فراسنجه­های خونی و pH  روده باریک مرغ‌های تخم­گذار تغذیه‌شده با جیره حاوی گندم از سن 54 تا 62 هفتگی با استفاده از تعداد 240 قطعه مرغ تخم­گذار های-لاین W-36 در قالب طرح کاملا تصادفی بین 40 قفس شامل پنج تیمار، هشت تکرار و شش پرنده در هر تکرار بررسی شد. جیره­های آزمایشی شامل الف- جیره شاهد بر پایه ذرت- کنجاله سویا، ب- جیره بر پایه گندم بدون افزودنی میکروبی، ج- جیره بر پایه گندم حاوی cfu/kg 109×1 باسیلوس ولزنسیس (B. velezensis)، د- جیره بر پایه گندم حاوی cfu/kg 105×1 قارچ تریکودرما (Trichoderma)، و- جیره بر پایه گندم با باسیلوس ولزنسیس+ تریکودرما (B.velezensis + Trichoderma)  بود.  نتایج نشان داد که افزودن باسیلوس ولزنسیس+ تریکودرما به جیره منجر به بهبود ضریب تبدیل خوراک نسبت به تیمار شاهد و تیمار بر پایه گندم و افزایش تولید تخم­مرغ و مصرف خوراک روزانه شد (05/0>p). هم‌چنین pH محتویات ژژنوم، ایلئوم و روده کور در تیمارهای حاوی میکروارگانیسم­ها کاهش یافت (05/0>p). پرندگان دریافت‌کننده میکروارگانیسم­ها سطح پلاسمایی کلسیم، فسفر و لیپوپروتئین با چگالی بالا (HDL) بالاتری را داشتند (05/0>p). افزودن باسیلوس ولزنسیس+ تریکودرما به جیره منجر به کاهش تعداد کلنی اشرشیاکلی و افزایش کلنی لاکتوباسیل­ها شد. براساس نتایج مطالعه حاضر، می­توان نتیجه گرفت که در مرغ‌های تخم‌گذار، افزودن باسیلوس ولزنسیس+ تریکودرما به جیره غذایی بر پایه گندم باعث بهبود عملکرد تولیدی، کاهش سطح کلسترول و تری­گلیسیرید پلاسما و بهبود میکروفلور روده می‌شود. 

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of direct-fed microbials on performance, pH values of gastrointestinal tract, blood parameters and small intestine microflora of laying hens fed wheat-soybean meal-based diet

نویسندگان [English]

  • zahra Mostafaie 1
  • Mehran Torki 2
  • Kianosh Chaghamirza 3
  • Rouhallah Sharifi 4

1 Corresponding Author, Animal Science Department, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran. E-mail: zmostafai@basu.ac.ir

2 Animal Science Department, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran. E-mail: torki@razi.ac.ir

3 Agronomy and Plant Breeding Department, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran. E-mail: cheghamirza@ razi.ac.ir

4 Plant Protection Department, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran E-mail: r.sharifi@razi.ac.ir

چکیده [English]

Introduction: Corn and wheat are the main sources of energy in poultry diets worldwide, although due to its availability, wheat can be a good substitute for corn in the diet of broilers and laying hens. However, the inclusion level of wheat is limited often because wheatbased diet contained higher levels ofsoluble non-starch polysaccharidesThe arabinoxylans are the main NSP in wheat that increasing the viscosity of the digesta and reducing nutrient digestibility of direct-fed microbial (DFM) supplements include Bacillus velezensis, Trichoderma, and Saccharomyces cerevisiae, which are used as biological control agents due to their high ability to suppress disease agents and inhibit the proliferation of intestinal pathogens.
Materials and Methods: The total number of 240 Hy-Line W-36 laying hens were randomly distributed between 40 cages, and the five experimental diets including (A) corn-soybean meal-based control diet, (B) wheat-based diet with no microbial additive, (C) wheat-based diet with 1Í109 cfu/kg Bacillus velezensis, (D) wheat-based diet with 1Í105 cfu/kg Trichoderma and (E) wheat-based diet with B. velezensis + Trichoderma were assigned to hens with 8 replicate cages per diet and 6 hens per each replicate.
Results and Discussion: The results showed that the addition of B. velezensis + Trichoderma to the diet led to an improvement in the feed conversion ratio (FCR),  and increased egg production (EP) and feed intake (FI; P<0.05)., Also, the pH of the contents of the jejunum, ileum and caecum was reduced in the treatments containing microorganisms(P<0.05). The DFMs enhanced the efficiency of nutrient adsorption and utilization, predominantly ascribable to the production of exogenous enzymes by the test probiotic. Prebiotics work as feed for the intestinal microflora and could stimulate the fermentation rate which increased the production of short chain fatty acids and reduced luminal pH. Hens fed microbe-added diets had higher plasma levels of calcium (Ca), phosphorus (P) and high-density lipoprotein (P<0.05). The addition of B. velezensis + Trichoderma to the diet, modulated the ileal and caecal microflora composition by decreasing the numbers of Escherichia coli and increasing the numbers of Lactobacilli. Dietary supplemented by B. velezensis and Trichoderma could increase plasma Ca and P compared to other treatments, which can be interpreted as probiotics increase the rate of fermentation and reduce intestinal pH, leads to better absorption of Ca and P from the intestine. Improved intestinal morphology characteristics were observed in hens fed the microbe-added diets (P<0.05). These beneficial effects were directly associated with decrease in total microbes, E. coli and Salmonella and enhance lactic acid bacteria of cecal. Consistently, addition of B. subtilis manipulated the gut ecosystem toward beneficial bacteria and enhanced the development and health of gastrointestinal tract. The positive effects of DFMs on eggshell quality can be attributed to the enhancement of gut health and intestinal tract microflora environment. Also, modifying the gut microflora composition by promoting the growth of beneficial microflora (such as lactic acid bacteria) and inhibiting the growth of Salmonella, E. coli, and Clostridium perfringens is beneficial effects of DFMs. The present study confirmed B. velezensis and Trichoderma enhanced the colonization of the beneficial bacteria and reduced the colonization of enteric bacteria. Maintaining a healthy intestine by probiotics and prebiotics will improve the ability of birds to overcome the disease and enhance their performance. Highest count of aerobic bacteria, lactobacilli, anaerobic bacteria, and E. coli were associated with Trichoderma reesei
Conclusion: Based on the results of the current study, it can be concluded that adding B. velezensis + Trichoderma to the wheat-based diets improve performance of laying hens, decrease plasma levels of cholesterol, triglyceride, and LDL and the intestinal pH and number of E. coli.

کلیدواژه‌ها [English]

  • Bacillus velezensis: Intestinal health
  • : Laying hen: Trichoderma
  • : Wheat
نعمتی، محمدحسین؛ حاجی‌لو، مصطفی؛ حسینی، سید عبداله و موسوی سیدسعید (1400). مقایسه اثر پروبیوتیک بیوپلاس B2 و پری‌بیوتیک گالاکتوالیگوساکارید بر عملکرد، کیفیت تخم‌مرغ تولیدی و برخی فراسنجه‌های خونی مرغ‌های تخم‌گذار تجاری. تولیدات دامی، 23(4)، 501-514.‎
 
References
Abdelqader, A., Irshaid, R., & Al-Fataftah, A. R. (2013). Effects of dietary probiotic inclusion on performance, eggshell quality, cecal microflora composition, and tibia traits of laying hens in the late phase of production. Tropical Animal Health And Production, 45(4), 1017-1024. https://doi.org/10.1007/s11250-012-0326-7.
 Bozkurt, M., Kucukyılmaz, K., Catlı, A.U., & Cınar, M. (2009). The effect of single or combined dietary supplementation of prebiotics, organic acid and probiotics on performance and slaughter characteristics of broilers. South African Journal of Animal Science, 39 (3), 302-304.
Chen, W., Wang, S., Xu, R., Xia, W., Ruan, D., Zhang, Y., & Zheng, C. (2021). Effects of dietary barley inclusion and glucanase supplementation on the production performance, egg quality and digestive functions in laying ducks. Animal Nutrition, 7(1), 176-184. https://doi.org/10.1016/j.aninu.2020.06.011.
Chen, Y.C., Nakthong, C., & Chen, T.C. (2005). Improvement of laying hen performance by dietary prebiotic chicory oligofructose and inulin. International Journal Poultry. Science, 4 103–108. https://10.3923/ijps.2005.103.108
CHOCT, M. (2015). Fibre-Chemistry and Functions in Poultry Nutrition. Paper presented at the LII Simposio Científico de Avicultura, Málaga, Spain, 29th Oct.
Engberg, R. M., Hedemann, M. S., Steenfeldt, S., & Jensen, B. B. (2004). Influence of whole wheat and xylanase on broiler performance and microbial composition and activity in the digestive tract. Poultry Science, 83(6), 925-938. https://doi.org/10.1093/ps/83.6.925.
Ferd, D.J. (1974). The effect of microflora on gastrointestinal pH in the chick. Poultry
Science, 53, 115-131.
Ferket, P. R. (2011). Nutrition-disease interactions regarding gut health in chickens. In Proceeding 18th European Symposium on Poultry Nutrition. Cesme, Izmir, Turkey.
Fukushima, M., & Nakano, M. (1995). The effect of a probiotic on faecal and liver lipid classes in rats. British Journal of Nutrition, 73(5), 701-710. https://doi.org/10.1079/BJN19950074.
Fuller, R., & Gibson, G. R. (1998). Probiotics and prebiotics: microflora management for improved gut health. Clinical Microbiology And Infection, 4(9), 477-480.
Gaggìa, F., Mattarelli, P., & Biavati, B. (2010). Probiotics and prebiotics in animal feeding for safe food production. International Journal Of Food Microbiology, 141, S15-S28. https://doi.org/10.1016/j.ijfoodmicro.2010.02.031.
Gao, X. Y., Liu, Y., Miao, L. L., Li, E. W., Sun, G. X., Liu, Y., & Liu, Z. P. (2017). Characterization and mechanism of anti-Aeromonas salmonicida activity of a marine probiotic strain, Bacillus velezensis V4. Applied Microbiology And Biotechnology, 101, 3759-3768.
Hadorn, R., & Wiedmer, H. (2001). Effect of an enzyme complex in a wheat-based diet on performance of male and female broilers. Journal of Applied Poultry Research, 10(4), 340-346.‏
Jasim, M.S. (2018). Use trichoderma harzianum and saccharomyces cerevisiae in Solid State Fermentation for Wheat Bran as Productive Performance Prebiotic of Layer Hens. Iraqi Poultry Sciences Journal, 12(2).
Karimi, A. (2022). Effect of Probiotics (direct-fed microbials) in poultry production: A Comprehensive Review. Revista Electronica de Veterinaria, 72-82. https://doi.org/10.3390/ani10101863.
Lee, K. W., Lee, S. H., Lillehoj, H. S., Li, G. X., Jang, S. I., Babu, U. S., ... & Siragusa, G. R. (2010). Effects of direct-fed microbials on growth performance, gut morphometry, and immune characteristics in broiler chickens. 10.3382/ps.2009-00418
Lee, S. H., Hosseindoust, A., Laxman Ingale, S., Rathi, P. C., Yoon, S. Y., Choi, J. W., & Kim, J. S. (2020). Thermostable xylanase derived from Trichoderma citrinoviride increases growth performance and non-starch polysaccharide degradation in broiler chickens. British Poultry Science, 61(1), 57-62. https://doi.org/10.1080/00071668.2019.1673316
Li, Y., Xu, Q., Huang, Z., Lv, L., Liu, X., Yin, C., Yan, H., & Yuan, J. (2016). Effect of Bacillus subtilis cgmcc 1.1086 on the growth performance and intestinal microbiota of broilers. Journal Applied Microbiology, 120(1), 195-204.  https://doi.org/10.1111/jam.12972
Macfarlane, G. T., & Cummings, J. H. (2002). Probiotics, infection and immunity. Current Opinion in Infectious Diseases, 15(5), 501-506.
Mirzaie, S., Zaghari, M., Aminzadeh, S., Shivazad, M., & Mateos, G. G. (2012). Effects of wheat inclusion and xylanase supplementation of the diet on productive performance, nutrient retention, and endogenous intestinal enzyme activity of laying hens. Poultry Science, 91(2), 413-425. https://doi.org/10.3382/ps.2011-01686
Naderi Sahami Zamir, S., Mirzaie Goudarzi, S., Saki, A. A., & Zamani, P. (2021). Effect of different levels of canola meal and protease enzyme on performance, egg quality traits and nutrient digestibility in laying hens. Animal Production Research, 9(4), 33-45. https://10.22124/AR.2021.15020.1474
Nemati, M. H., Hajilo, M., Hosseini, S. A., & Mosavi, S. S. (2021). Comparison of the effect of Bioplus B2 probiotic and galacto oligosaccharide prebiotic on performance, egg quality and some blood parameters of commercial laying hens. Animal Production, 23(4), 549-559. (inPersian)
Nguyen, H. T., Wu, S. B., Bedford, M. R., Nguyen, X. H., & Morgan, N. K. (2021). Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens. British Poultry Science, 1-11. https://doi.org/10.1080/00071668.2021.2003754
Omwango, E. O., Njagi, E. N. M., Orinda, G. O., & Wanjau, R. N. (2013). Nutrient enrichment of pineapple waste using Aspergillus niger and Trichoderma viride by solid state fermentation. African Journal of Biotechnology, 12(43), 6193-6196.
Panda, A.K., Reddy, M.R., Rama Rao, S.V., Raju, M.V.L.N., and Paraharaj, N.K. (2000). Growth, carcass characteristics, immunocomponence and response to Escherchia coli of broiler fed diets with various level of probiotic. Archive for Geflugel Customer, 64, 152-156.
pang, Y., & Applegate, T. J. (2007). Effects of dietary copper sup-plementation and copper source on digesta pH, calcium, zinc, and copper complex size in the gastrointestinal tract of the broiler chicken. Poultry Science, 86, 531-537. https://doi.org/10.1093/ps/86.3.531.
Salim, H. M., Kang, H. K., Akter, N., Kim, D. W., Kim, J. H., Kim, M. J., ... & Kim, W. K. (2013). Supplementation of direct-fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens. Poultry Science, 92(8), 2084-2090.‏
Scholz-Ahrens, K. E., Ade, P., Marten, B., Weber, P., Timm, W., Aςil, Y., . & Schrezenmeir, J. (2007). Prebiotics, probiotics, and synbiotics affect mineral absorption, bone mineral content, and bone structure. The Journal of Nutrition, 137(3), 838S-846S. https://doi.org/10.1093/jn/137.3.838S
Shalaei, M., Hosseini, S. M., & Zergani, E. (2014). Effect of different supplements on eggshell quality, some characteristics of gastrointestinal tract and performance of laying hens. In Veterinary Research Forum. Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Singh, Y., Molan, A. L., & Ravindran, V. (2019). Influence of the method of whole wheat inclusion on performance and caecal microbiota profile of broiler chickens. Journal of Applied Animal Nutritionhttps://doi.org/10.1017/jan.2019.3
Statistical Analytical Systems. (2012). SAS 9.4 for Windows x64 Based Systems. SAS Institute Inc., Cary, NC 27513, USA.
Tasirnafas, M., Karimi, K., Asgari Jafarabadi, G., Seidavi, A., & Noorbakhsh, F. (2020). Extraction and purification of β-glucanase from bovine rumen fungus Trichoderma reesei and its effect on performance, carcass characteristics, microbial flora, plasma biochemical parameters, and immunity in a local broiler hybrid Golpayegan-Ross. Tropical Animal Health and Production, 52(4), 1833-1843. https://doi.org/10.1007/s11250-019-02186-5
Trichoderma and probiotics: scope for future research. Physiological and Molecular Plant Pathology, 100, 84-96. https://doi.org/10.1016/j.pmpp.2017.07.005.
Upadhaya, S. D., Rudeaux, F., & Kim, I. H. (2019). Effects of inclusion of Bacillus subtilis (Gallipro) to energy-and protein-reduced diet on growth performance, nutrient digestibility, and meat quality and gas emission in broilers. Poultry Science, 98(5), 2169-2178.
Wang, J. P., & Kim, I. H. (2011). Effect of caprylic acid and Yucca schidigera extract on production performance, egg quality, blood characteristics, and excreta microflora in laying hens. British poultry Science, 52(6), 711-717. https://doi.org/10.1080/00071668.2011.635638
Wang, J. P., Lee, J. H., Yoo, J. S., Cho, J. H., Kim, H. J., & Kim, I. H. (2010). Effects of phenyllactic acid on growth performance, intestinal microbiota, relative organ weight, blood characteristics, and meat quality of broiler chicks. Poultry Science, 89(7), 1549-1555. https://doi.org/10.3382/ps.2009-00235
Ye, M., Wei, C., Khalid, A., Hu, Q., Yang, R., Dai, B., ... & Wang, Z. (2020). Effect of Bacillus velezensis to substitute in-feed antibiotics on the production, blood biochemistry and egg quality indices of laying hens. BMC veterinary research, 16, 1-8.
Ye, M., Wei, C., Khalid, A., Hu, Q., Yang, R., Dai, B., ... & Wang, Z. (2020). Effect of Bacillus velezensis to substitute in-feed antibiotics on the production, blood biochemistry and egg quality indices of laying hens. BMC Veterinary Research, 16(1), 1-8. https://doi.org/10.1186/s12917-020-02570-6.
Zhang, J.L., Xie, Q.M., Ji, J., Yang, W.H., Wu, Y.B., Li, C., & Bi, Y.Z. (2012). Different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens. Poultry Science, 91(11), 2755-2760.