نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانش آموخته دکتری حرفه ای، دانشکده دامپزشکی، دانشگاه لرستان
2 عضو هیآت علمی دانشگاه لرستان، تخصص: مامایی و بیماری‌های تولیدمثل دام/ ارزیابی اسپرم در حیوانات اهلی و موش‌ها/ سونوگرافی حیوانات
3 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه لرستان، خرم آباد، ایران
4 استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان
چکیده
این طرح با هدف بررسی تغییرات در برخی از فراسنجه های خونی در 15 رأس میش نژاد لری در طی آبستنی و مقایسه آن با یک ماه قبل از آبستنی و دوره شیردهی انجام شد. نمونه‌های خون قبل از جفت گیری طبیعی به عنوان شاهد، پایان هر سه ماه آبستنی و دو هفته بعد از زایش در دوره شیردهی از ورید وداج اخذ شدند و بوسیله دستگاه سل کانتر دامپزشکی، شمارش کامل سلول های خونی هر نمونه ارزیابی شد. در گسترش‌ها رنگ آمیزی گیمسا و شمارش تفریقی لکوسیت ها انجام شد و مقادیر پروتئین تام پلاسما به روش رفرکتومتری ارزیابی شد. تعداد گلبول‌های قرمز و درصد هماتوکریت‌ها در دوره شیردهی نسبت به دوره آبستنی کاهش معنی دار داشتند (0/05˂P). میزان حجم متوسط گلبولی در همه دوره‌ها در یک طیف گزارش شد. کاهش مقادیر متوسط میزان هموگلوبین گلبول‌های قرمز، غلظت متوسط هموگلوبین در گلبول‌های قرمز و تعداد پلاکت‌ها در آبستنی نسبت به پیش از جفت‌گیری مشاهده شد. تعداد لکوسیت‌ها و نوتروفیل‌ها در حوالی زایمان افزایش و بعد از زایمان کاهش یافتند. در طی آبستنی تعداد ائوزینوفیل‌ها به صورت معنی‌دار افزایش و بعد از زایمان کاهش یافتند (0/05˂P). تعداد مونوسیت‌ها و لنفوسیت‌ها در اواخر آبستنی افزایش و در دوره شیرواری کاهش یافتند (0/05˂P). میزان پروتئین‌تام پلاسما در بازه روزهای 150-100 آبستنی به صورت معنی دار کاهش یافت (0/05˂P). به این ترتیب نتیجه می‌شود که نظارت بر تغییرات فراسنجه های خونی میش‌های لری، تنظیم جیره غذایی و اقدامات پیشگیرانه برای ممانعت از اختلالات خونی نظیر کم خونی و ترومبوسیتوپنی الزامی می باشد.
کلیدواژه‌ها
عنوان مقاله [English]
Evaluation of changes in the leukogram in pregnant Lori ewes before pregnancy, during pregnancy and lactation
نویسندگان [English]
- fatemeh sarlak 1
- Arash Kheradmand 2
- Alireza Rocky 3
- Majid Khaldari 4
1 Professional PhD student, Faculty of Veterinary Medicine, Lorestan University
2 Lorestan University faculty member, specialty: Midwifery and reproductive diseases of livestock / Sperm evaluation in domestic animals and mice / Ultrasound of domestic animals / Mastitis / The effect of antioxidants on reproduction
3 Department of Clinical Pathology, Lorestan University, Khoramabad, Iran
4 (Assistant Professor, Department of Animal Sciences, Faculty of Agriculture, Lorestan University, Khorramabad, Iran)
چکیده [English]
The aim of this study was to investigate the changes in some blood parameters in 15 Lori ewes during pregnancy and compare it with one month before pregnancy and lactation. Blood samples were taken from the jagulur vein before normal mating as a control, at the end of every three months of pregnancy and two weeks after delivery during lactation, and the complete blood cell count of each sample was evaluated by a veterinary cell counter. In extensions, Giemsa staining and differential white blood cell count were performed and total plasma protein values were evaluated by refractometry. The number of red blood cells and the percentage of hematocrits during lactation were significantly reduced compared to pregnancy (P˂0.05). Mean cellular volume was reported in all periods in one spectrum. Decreases in mean hemoglobin levels of red blood cells, mean hemoglobin concentrations in red blood cells and platelet count in pregnancy were observed compared to before mating. The number of leukocytes and neutrophils increased around delivery and decreased after delivery. During pregnancy, the number of eosinophils increased significantly and decreased after delivery (P˂0.05). The number of monocytes and lymphocytes increased in late pregnancy and decreased during lactation (P˂0.05). The amount of total protein in the plasma decreased significantly from 150-100 days of gestation (P˂0.05). Thus, it is concluded that monitoring of changes in blood parameters of Lori ewes, dietary adjustment and preventive measures are necessary to prevent blood disorders such as anemia and thrombocytopenia.
کلیدواژه‌ها [English]
- Blood parameters
- Lori breed
- Leukogram
- Pregnancy
- Pregnant ewes
- Total protein
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