Document Type : Research Paper
Authors
1 Professional PhD student, Faculty of Veterinary Medicine, Lorestan University
2 Lorestan University faculty member, specialty: Midwifery and reproductive diseases of livestock / Sperm evaluation in domestic animals and mice / Ultrasound of domestic animals / Mastitis / The effect of antioxidants on reproduction
3 Department of Clinical Pathology, Lorestan University, Khoramabad, Iran
4 (Assistant Professor, Department of Animal Sciences, Faculty of Agriculture, Lorestan University, Khorramabad, Iran)
Abstract
The aim of this study was to investigate the changes in some blood parameters in 15 Lori ewes during pregnancy and compare it with one month before pregnancy and lactation. Blood samples were taken from the jagulur vein before normal mating as a control, at the end of every three months of pregnancy and two weeks after delivery during lactation, and the complete blood cell count of each sample was evaluated by a veterinary cell counter. In extensions, Giemsa staining and differential white blood cell count were performed and total plasma protein values were evaluated by refractometry. The number of red blood cells and the percentage of hematocrits during lactation were significantly reduced compared to pregnancy (P˂0.05). Mean cellular volume was reported in all periods in one spectrum. Decreases in mean hemoglobin levels of red blood cells, mean hemoglobin concentrations in red blood cells and platelet count in pregnancy were observed compared to before mating. The number of leukocytes and neutrophils increased around delivery and decreased after delivery. During pregnancy, the number of eosinophils increased significantly and decreased after delivery (P˂0.05). The number of monocytes and lymphocytes increased in late pregnancy and decreased during lactation (P˂0.05). The amount of total protein in the plasma decreased significantly from 150-100 days of gestation (P˂0.05). Thus, it is concluded that monitoring of changes in blood parameters of Lori ewes, dietary adjustment and preventive measures are necessary to prevent blood disorders such as anemia and thrombocytopenia.
Keywords
of Tehran Press, (1): 43-44. (In Persian)
2. Ahmadi M, Kafi M and Ghodrat M (2005)
Crystallization and the number of neutrophils
increase in the cervical mucus as parturition
approaches in dairy cows. Comparative
Clinical Pathology, 14(2): 72-75.
3. AL-Hadithy H and Suleiman J (2014) The
hematological parameters in clinically normal
lactating and ewes affected with mastitis. Kufa
Journal for Veterinary Medical Sciences, 5(2).
4. Alonso A, De Teresa R, Garcia M, Gonzalez J
and Vallejo M (1997) The effects of age and
reproductive status on serum and blood
parameters in Merino breed sheep. Journal of
Veterinary Medicine Series A, 44(1‐10):223-231.
5. Ayoub M, El-Khouly A and Mohamed T
(2003) Some hematological and biochemical
parameters and steroid hormone levels in the
one-humped camel during different
physiological conditions. Emirates Journal of
Food and Agriculture, 44-55.
6. Azab M and Abdel-Maksoud A (1999)
Changes in some hematological and
biochemical parameters during prepartum and
postpartum periods in female Baladi
goats. Small Ruminant Research, 34(1): 77-85.
7. Badawi N and AL-Hadithy H (2014) The
hematological parameters in clinically healthy
Iraqi Awassi sheep. Platelets, 32: 0-12.
8. Barbazanges A, Piazza P, Le Moal M and
Maccari S (1996) Maternal glucocorticoid
secretion mediates long-term effects of
prenatal stress. Journal of Neuroscience,
16(12): 3943-3949.
9. Chandra S, Tripathi A, Mishra S, Amzarul M
and Vaish K (2012) Physiological changes in
hematological parameters during pregnancy.
Indian journal of hematology and blood
transfusion, 28(3): 144-146.
10. Davson H (1960) Textbook of general
physiology. Academic Medicine, 35(2): 204.
11. Iriadam M (2007) Variation in certain
hematological and biochemical parameters
during the peri-partum period in Kilis
does. Small Ruminant Research, 73(1-3): 54-57.
12. Jain N (1993) Essentials of veterinary
hematology, comparative hematology of
common domestic animals. Philadelphia, PA:
44-46.
13. Jainudeen M and Hafez E (2000) Gestation,
prenatal physiology, and parturition.
Reproduction in farm animals, 140-155.
14. Kandiel M, El-Khaiat H and Mahmoud K
(2016) Changes in some hematobiochemical
and hormonal profile in Barki sheep with
various reproductive statuses. Small Ruminant
Research, 136: 87-95.
15. Karen A, Beckers J, Sulon J, de Sousa N,
Szabados K, Reczigel J and Szenci O (2003)
Early pregnancy diagnosis in sheep by
progesterone and pregnancy-associated
glycoprotein tests. Theriogenology, 59(9):
1941-1948.
16. Kayhanian Sh (2011) Evaluation of the
frequency of pregnancy thrombocytopenia in
patients referred to Tonekabon health centers.
Journal of Ardabil University of Medical
Sciences, 4: 10. (In Persian)
17. Liberg P (1977) Agarose gel electrophoretic
fractionation of serum proteins in adult
cattle. Acta Veterinaria Scandinavica, 18(1):
40-53.
18. Makrides M, Crowther C, Gibson R, Gibson R
and Skeaff C (2003) Efficacy and tolerability
of low-dose iron supplements during
pregnancy: a randomized controlled trial. The
American journal of clinical nutrition, 78(1):
145-153.
19. Nazari Sh (2017) Blood textbook. Shahid
Beheshti University of Medical Sciences
Publications, (1): 14 (In Persian)
20. Norouzi M and Khalaj V (1990) Clinical
Biochemistry (Translation). Daneshpajooh
Publications. First (1): 349-348. (In Persian)
21. Picciano M (2003) Pregnancy and lactation:
physiological adjustments, nutritional
requirements and the role of dietary
supplements. The Journal of nutrition, 133(6):
1997S-2002S.
22. Sharma A, Kumar P, Singh M and Vasishta N
(2015) Haemato-biochemical and endocrine
profiling of north western Himalayan Gaddi
sheep during various physiological/ reproductive
phases. Open veterinary journal, 5(2): 103-107.
23. Yılmaz B (2000) Blood and Blood circulatory,
pp: 307-308.
)