نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار، هیأت علمی بخش تحقیقات علوم دامی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان چهارمحال و بختیاری.
2 استاد،دانشگاه رازی کرمانشاه - دانشکده کشاورزی - گروه علوم دامی
3 استادیار،گروه میکروبشناسی و بیوتکنولوژی، موسسه مکس رابنر، کیل، آلمان.
4 استاد،گروه علوم دامی دانشگاه ایلینوی. آمریکا
5 دانشیار،گروه علوم دامی و صنایع غذایی دانشگاه تگزاس- تک. تگزاس. آمریکا
چکیده
هدف از این پژوهش مطالعه اثر جیره حاوی کربوهیدرات‌های غیرالیافی زیاد (40/7 درصد) بر بیان برخی از ژن‌های مرتبط با التهاب و سیستم ایمنی ذاتی در گاوهای شیرده جرسی بود. برای انجام آزمایش از هفت رأس گاو چند شکم‌زا (0/5±2/5) جرسی با میانگین روزهای شیردهی 3±71 و میانگین تولید شیر 6/6 ± 28 کیلوگرم استفاده شد. گاو در دو هفته اول آزمایش جیره شاهد( حاوی 34 درصد کربوهیدرات غیرالیافی ) و در هفته سوم، جیره حاوی کربوهیدرات غیرالیافی زیاد( 40/7 درصد کربوهیدرات غیرالیافی) دریافت کردند. نمونه‌های خون در روز صفر (انتهای روز 14، که گاوها جیره شاهد دریافت کرده بودند) و روزهای چهارم و هفتم (پس از تغییر جیره) تهیه شد. بیان نسبی هفت ژن مرتبط با سیستم ایمنی شامل ژن‌های TNF-α، IL1β،TLR2 ، SELL، CD74، MNDA و TGFβ اندازه‌گیری شد. بر اساس نتایج حاصل، مصرف جیره حاوی کربوهیدرات‌های غیرالیافی زیاد موجب القاء التهاب و تأثیر بر سیستم ایمنی ذاتی می شود.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of high non fibrous carbohydrates diet on some inflammation related genes expression in lactating Jersey cows
نویسندگان [English]
- Golnaz Taasoli 1
- Farrokh Kafilzadeh 2
- Darab Ghadimi 3
- Juan Loor 4
- Micheal Ballou 5
1 Department of Animal Science, Chahatmahal Bakhtiari Agricultural and Natural Resources Research and Education Center
2 Department of animal science, Razi University
3 Department of Microbiology and Biotechnology, Max Rubner-Institute, Kiel, Germany.
4 Department of Animal Science, University of Illinois, USA.
5 Department of Animal and Feed Science, Texas Tech University, Texas, USA.
چکیده [English]
The objective of this experiment was to study the effect of diet containing high (40.7 %) non fibrous carbohydrates on some inflammatory and native immunity related genes expression in lactating Jersey cows. Seven lactating multiparous (2.5 ± 0.5) Jersey cows with 71 ± 3 days in milk and 28 ± 6.6 kg of average milk production were used. All cows received a control diet containing low (34.3 percentage of dry matter) non fibrous carbohydrates content for the first 14 days and then were were switched to a diet containing high (40.7 percentage of dry matter) non fibrous carbohydrates content for one week. Blood samples were collected on day 0 (After 14 days of feeding control diet) and days 4 and 7 after feeding high NFC diet. Relative gene expression of seven genes (IL1-β, TNF-α, MNDA, CD74, TLR2, SELECTIN, TGF1-β) were measured. The results showed that gene expression of the pro-inflammatory cytokines (IL1-β and TNF-α) and anti-inflammatory proteins (MNDA, D74, TLR2, SELECTIN, TGF1-β) was down regulated during feeding of high NFC diet (p < 0.05). These data indicated that feeding a high non-fibrous carbohydrates diet caused inflammation and affected immunity system in lactating Jersey cows.
کلیدواژه‌ها [English]
- Carbohydrates
- Cytokine
- Dairy cow
- Gene
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- Inflammation
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