نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی-دانشکده کشاورزی-دانشگاه رازی-کرمانشاه-ایران

2 دانشگاه رازی، تخصص: تغدیه دام/ مواد معدنی

3 پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، تخصص: فیزیولوژی دام

4 دانشگاه علوم کشاورزی و منابع طبیعی ساری، تخصص: غذا و تغذیه نشخوارکنندگان/ بیوشیمی و کنترل کیفی مواد غذایی

چکیده

به منظور تعیین اثرات مکمل خوراکی اسید لینولئیک مزدوج (CLA) محافظت شده و مکمل تزریقی سلنیوم و ویتامین E (E-Se) بر سیستم ایمنی گاوهای شیری و گوساله های نوزاد آنها، ۴۰ رأس گاو شیری هلشتاین شکم دوم و بالاتر در یکی از گاوداری‌های صنعتی استان قزوین در سال 1394 مورد استفاده قرار گرفت. حیوانات در یک طرح کاملاً تصادفی با آرایش فاکتوریل به چهار گروه با 10 تکرار، تقسیم شدند. گروه های ازمایشی شامل جیره پایه (کنترل)، جیره پایه به علاوه مکمل تزریقی E-Se ، جیره پایه به علاوه 100 گرم مکمل CLA در روز و جیره پایه به علاوه 100 گرم مکمل CLA همراه مکمل تزریقی E-Se بود. مکمل E-Se به میزان 40 سی‌سی در روزهای 21-، 1، 14، 28 و 42 نسبت به روز زایش به صورت عضلانی به هر گاو تزریق شد. نمونه‌های خون از همه گاوها در روزهای تزریق گرفته شد. خون گیری از گوساله ها در روز تولد قبل از خوردن آغوز و سن هفت روزگی انجام شد. افزودن مکمل CLA تأثیر معنی‌داری بر میانگین غلظت ایمونوگلوبولین G ((IgG و وضعیت کل آنتی اکسیدانی (TAS) پلاسمای گوساله‌ها در سنین یک و هفت روزگی نداشت. اثر مکمل E-Se بر میزان TAS پلاسمای گوساله ها در سن 7 روزگی معنی دار بود (05/0>P). افزودن مکمل CLA و مکمل E-Se تأثیر معنی داری بر میانگین غلظت IgG ، MDA و TAS آغوز گاوها نداشت. افزودن مکمل CLA و مکمل E-Se تأثیر معنی داری بر میزان TAS خون و میانگین فراسنجه های خون شناسی گاوها نداشت. بر اساس نتایج حاصل، استفاده از اسید لینولئیک مزدوج در دوره انتقال اثری بر سیستم ایمنی گاوها و گوساله های متولد شده ندارد. مکمل تزریقی سلنیوم و ویتامین E وضعیت کل آنتی اکسیدانی خون گوساله های متولد شده را بهبود می بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Dietary Conjugated linoleic Acid (CLA) with or without injectable Se and VE supplement on immune system of lactating dairycows and their calves

نویسندگان [English]

  • zabih allah abdolmaleki 1
  • Manouchehr Souri 1
  • mohamad mehdi moeini 2
  • armin towhidi 3
  • Yadollah Chashnidel 4

1 Departemant of Animal sciences, Faculty of Agriculture, Razi University, iran

2 Depart. of Animal sciences, Faculty of Agriculture, Razi University

3 Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

4 Department of Animal sciences sari agricultural science and natural resources university.

چکیده [English]

Forty multiparous Holstein cows were used to investigate the effect of supplementary rumen protected Conjugated linoleic Acid (CLA) with injectable Se and vitamin E (E-Se) supplement on immune system in Holstein cows and their calve in a commercial dairy farm at Ghazvin province. The animals were divided in four experimental groups in a complete randomize design based on factorial. The experimental groups were: basal diet (Control), basal diet plus injectable E-Se Supplement, basal diet plus CLA 100 g/day per each cow) and basal diet plus CLA 100 g/day per each cow and Injectable E-Se Supplement. The dose of injectable E-Se supplement was 40 ml/cow. The injection has done muscularly in each cow in days, -21, 1, 14, 28 and 42 after calving. Blood Samples of cows collected at days of injection. Blood samples of calves collected at birthday pre feeding colostrum and 7 days after birth. The result showed CLA supplement had no significantly effect on mean concentration of Immunoglobulin G (IgG), Total antioxidant status (TAS) and Malondialdehyde (MDA) in calves on days 1 and 7 (P>0.05). Effect of injectable E-Se Supplement on TAS concentration of Calve's Plasma had was significant (P>.05). CLA and injectable E-Se Supplements had no significant effect on mean concentration of IgG, TAS and MDA of cow`s colostrum (P>0.05). The CLA and E-Se supplements had no significant effect on TAS concentration and hematology Parameters of dairy cow (P>0.05). Based on acquried result use of CLA supplement in transition period had no effect on immune system of cows and their calves. Supplementary injection of E-Se significantly improved total antioxidant status of newborn calves.

کلیدواژه‌ها [English]

  • Colostrum
  • Immunoglobulin G (IgG)
  • Malondialdehyde (MDA)
  • Total antioxidant status (TAS)
  • White blood cells

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