بررسی ارتباط چند‌‌شکلی تک نوکلئوتیدی ژن STAT1 با صفات تولید شیر در گاوهای براون سوئیس

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، گروه علوم دامی، دانشکده کشاورزی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران

2 استادیار، گروه علوم دامی، دانشکده کشاورزی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران

چکیده

چند­شکلی تک­نوکلئوتیدی ژن STAT1 با روش PCR-RFLP و ارتباط آن با صفات تولید شیر در گاو­های شیری نژاد براون­سوئیس بررسی شد. تعداد ۹۸ رأس گاو شیری نژاد براون­سوئیس به طور تصادفی از گاوداری­های صنعتی مشهد انتخاب و خون­گیری ازورید دمی انجام شد. استخراج DNA با استفاده از کیت آماده انجام شد. یک قطعه دارای ۳۱۴ جفت باز در ناحیه 3'UTR ژن STAT1 با روش PCR تکثیر و سپس قطعه مورد نظر توسط آنزیم برشی Pag1 هضم شد. فراوانی­های ژنوتیپی و ژنی با نرم­افزار POPGENE (نسخه 31/1) برآورد شد. برای تجزیه و تحلیل آماری از نرم­افزار آماری SAS استفاده شد. میانگین صفات با روش دانکن مقایسه شد. فراوانی ژنوتیپ­های TT، TC و  CCبه ترتیب 23/0 ، 25/0 و 5۲/0 بود. فراوانی آلل­های T و C به ترتیب 35۵/0 و 6۴۵/0 برآورد شد. هتروزیگوسیتی مورد انتظار معادل ۰/۴۵۸ برآورد شد. آزمون مربع کای نشان داد که جمعیت مورد مطالعه در تعادل هاردی­وینبرگ نمی­باشد (05/0P<). تفاوت میانگین الگو­های ژنوتیپی در جایگاه ژن STAT1 برای صفات شیر (شامل تولید شیر، درصد و مقدار چربی شیر و مقدار پروتئین شیر) معنی­دار نبود، ولی برای صفت درصد پروتئین شیر معنی­دار بود (05/0P<). میانگین تولید شیر و مقادیر چربی و پروتئین برای ژنوتیپ CC نسبت به دو ژنوتیپ دیگر بیشتر بود. با توجه به نتایج حاصل، آلل T به عنوان آلل با فراوانی کم شناخته می­شود و وجود آلل C در ژنوتیپ گاو اثر مثبت بر صفات تولید شیر، مقدار چربی و پروتئین دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Association of single nucleotide polymorphism of STAT1 gene with milk production traits in Brown Swiss cattle

نویسندگان [English]

  • Hengameh Vafaee 1
  • Mojtaba Hosseinpour Mashhadi2 2
1 M.Sc. Graduate, Department of Animal Science, Faculty of Agriculture, Mashhad Branch, Islamic Azad University, Mashhad - Iran
2 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Mashhad Branch, Islamic Azad University, Mashhad - Iran
چکیده [English]

 This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety eight cows were collected randomly from tail vein. The DNA was extracted with Cinagene kit (PR881612C). Fragment of 314 bp of STAT1 gene was amplified by PCR and digested by Pag1 restriction enzyme. Genotype frequencies were calculated by PopGene software (version 1.31). The SAS 9.1 software was used for statistical analysis and the mean of traits was compared by Duncan test. The genotype frequencies of TT, TC and CC were 0.23, 0.25 and 0.52 respectively. The T and C allele frequency were 0.355 and 0.645 respectively. The expected heterozygosity was estimated 0.458. Result of Chi-square showed that the population was not in the Hardy Weinberg equilibrium (P<0.05). The means of traits for milk, percent and yield of fat and yield of protein were not significant but means of percent of protein was significant(P<0.05). The mean of milk, fat and protein yield traits for CC genotype were more than two other genotypes. The results of present study show that the T allele is minor frequency allele and C allele has positive effect on milk, fat and protein yield traits.

کلیدواژه‌ها [English]

  • Brown Swiss breed
  • Gene
  • Milk production traits
  • STAT1
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