نوع مقاله : مقاله پژوهشی
نویسنده
عضو هیآت علمی
چکیده
در این مطالعه، بررسی اثر غلظت‌های مختلف آنتی‌اکسیدان‌های آلفاتوکوفرول، آلبومین سرم گاوی (BSA) و ویتامین C بر شاخص‌های ارزیابی اسپرم بز متعاقب 48 ساعت نگهداری در دمای 5 درجه سانتی‌گراد بررسی شد. تیمارها شامل 7 گروه رقیق‌کننده حاوی آلفاتوکوفرول با دو غلظت 5 و 10 واحد، BSA با دو غلظت 4 و 8 میلی‌گرم در میلی‌لیتر، اسید آسکوربیک با غلظت‌های 3 و 6 میلی‌گرم در میلی‌لیتر و گروه شاهد بدون افزودن ماده آنتی‌اکسیدانی بودند. در زمان های صفر، 24 و 48 ساعت، تحرک کلی و پیشرونده و نیز سلامت غشای اسپرم‌ها ارزیابی شدند. تحرک اسپرم و سلامت غشاء هنگام استفاده از رقیق‌کننده BSA با غلظت 4 میلی‌گرم و ویتامین E با غلظت پنج واحد بیشتر از سایر رقیق‌کننده‌ها بود (05/0P<). اگرچه غلظت‌های بالاتر این 2 آنتی‌اکسیدان باعث افزایش کمتر شاخص‌های مورد ارزیابی شد، اما نسبت به گروه شاهد بهبود نسبی را نشان دادند (05/0P<). غلظت‌های مختلف اسید آسکوربیک بر هیچ‌یک از فراسنجه‌های ارزیابی اسپرم اثر نداشت. براساس نتایج تحقیق حاضر، افزودن BSA و ویتامین E به اسپرم بز در طی فرآیند نگهداری در دمای 5 درجه تحرک کلی و پیشرونده و نیز سلامت غشاء در طی 48 ساعت را بهبود می‌بخشد.
کلیدواژه‌ها
عنوان مقاله [English]
Protective effects of α-tocopherol, BSA and vitamin C on caprine sperm quality during incubation at 5° C
نویسنده [English]
- Arash Kheradmand
چکیده [English]
This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin C, and control group without any antioxidant. Total sperm motility, progressive motility and HOS test were assessed at h 0, 24 and 48. Using 4 mg of BSA and 5 IU of vitamin E increased sperm motility and plasma membrane integrity compared to other antioxidants (P<0.05). Although, higher concentrations of these antioxidants relatively enhanced sperm quality parameters, however, it was still greater than the control group (P<0.05). Different concentrations of vitamin C did not affect sperm quality parameters. According to the results of the present study, addition of BSA and vitamin E to buck semen improved total and progressive sperm motility as well as plasma membrane integrity during 48 h after incubation at 5 °C.
کلیدواژه‌ها [English]
- Antioxidant
- Buck
- Lipid Peroxidation
- Membrane integrity
- Sperm
- Anghel A, Zamfirescu S, Dragomir C, Nadolu D, Elena S and Florica B (2010) The effects of antioxidants on the cytological parameters of cryopreserved buck semen. Romanian Biotechnological Letters. 15: 26-32.
- Arthur GH, Noakes DE, Pearson H and Parkinson TJ (1996) Veterinary Reproduction and Obstetrics. 7th Edition. W.B. Saunders Company, London. Pp. 634-647.
- Arul-Murugan A, Gangadharan B and Mathur PP (2002) Antioxidative effect of fullerenol on goat epididymal spermatozoa. Asian Journal of Andrology. 4: 149-152.
- Aurich JE, Schonherr U, Hoppe H and Aurich C (1997) Effects of antioxidants on motility and membrane integrity of chilled stored stallion semen. Theriogenology. 48: 185-192.
- Ax RL, Dally MR, Didon BA, Lenz RW, Love CC, Varner DD, Hafez B and Bellin ME (2000) Artificial insemination. in: Reproduction in Farm Animals. 7th Edition. Lea and Febiger, Philadelphia. Pp. 376-389.
- Babaei H and Sivandi S (2014) The effects of in vitro supplementation of capacitation medium with ascorbic acid on kinetic parameters of caprine epididymal sperm. Comparative Clinical Pathology. 23: 63-68.
- Ball BA, Medina V, Gravance CG and Baumber J (2001) Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5ºC. Theriogenology. 56: 577-589.
- Barbas JB and Mascarenhas RD (2009) Cryopreservation of domestic animal sperm cells. Cell Tissue Bank. 10: 49-62.
- Chen Y, Foote RH and Brockett CC (1993) Effect of sucrose, trehalose, hypotaurine, taurine, and blood serum on survival of frozen bull sperm. Cryobiology. 30: 423-431.
- Gadea J, Garcıa-Vazquez F, Matas C, Gardon JC, Canovas S and Gumbao D (2005) Cooling and freezing of boar spermatozoa: supplementation of the freezing media with reduced glutathione preserves sperm function. Journal of Andrology. 26: 396-404.
- Gangadharan B, Arul Murugan A and Mathur PP (2001) Effect of methoxychlor on antioxidant system of goat epididymal sperm in vitro. Asian Journal of Andrology. 3: 285-288.
- Kheradmand A, Babaei H and Abshenas J (2006) Comparative evaluation of the effect of antioxidants on the chilled-stored ram semen. Iranian Journal of Veterinary Research. 7: 40-45.
- Memon AA, Wahid H, Rosnina Y, Goh YM, Ebrahimi M and Nadia FM (2013) Effect of Ascorbic Acid Concentrations, Methods of Cooling and Freezing on Boer Goat Semen Cryopreservation. Reproduction in Domestic Animals. 48: 325-330.
- Paulenz H, Soderquist L, Adnoy T, Soltun K, Saether PA and Fjellsoy KR (2005) Effect of cervical and vaginal insemination with liquid semen stored at room temperature on fertility of goats. Animals Reproduction Science. 86:
109-117. - Salamon S and Maxwell WM (2000) Storage of ram semen. Animal Reproduction Science. 62: 77-111.
- Salvador I, Yaniz J, Viudes-de-Castro MP, Gomez EA and Silvestre MA (2006) Effect of solid storage on caprine semen conservation at 5 ͦ C. Theriogenology. 66: 974-981.
- Sariozkan S, Turk G, Canturk F, Yay A, Eken A and Akcay A (2013) The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology. 67: 1-6.
- Silva SV, Soares AT, Batista AM, Almeida FC, Nunes JF, Peixoto CA and Guerra MMP (2013) Vitamin E (Trolox) addition to Tris-egg yolk extender preserves ram spermatozoon structure and kinematics after cryopreservation. Animal Reproduction Science. 137: 37-44.
- Sliwa L and Macura B (2005) Evaluation of cell membrane integrity of spermatozoa by hypoosmotic swelling test-water test in mice after intraperitoneal daidzein administration. Archives of Andrology. 51: 443-448.
- Swain DK, Swarnkar P, Kumar J and Yadav S (2012) Evaluation of in vitro longevity of caprine cauda epididymal sperms at different storage intervals of time. Indian Journal of Animal Science. 82: 1347-1350.
- Vishwanath R and Shannon P (2000) Storage of bovine semen in liquid and frozen state. Animal Reproduction Science. 62: 23-53.
- Usal O and Bucak MN (2007) Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed rem semen. Acta Veterinary. 76: 383-390.
- Usal O, Korkmaz T, Yavas I and Bucak NM (2006) Evaluation by hypoosmotic swelling –eosine test of cryopreserved bovine spermatozoa. Indian Veterinary Journal. 83: 557-559.
- Yamashiro H, Wang H, Yamashita Y, Kumamoto K and Terada T (2006) Enhanced freezability of goat spermatozoa collected into tubes containing extender supplemented with bovine serum albumin. Journal of Reproduction and Development. 52: 407-414.
)