بررسی اثرات حفاظتی آلفا توکوفرول، BSA و ویتامین C بر کیفیت اسپرم بز طی نگهداری در دمای پنج درجه سانتی گراد

نوع مقاله : مقاله پژوهشی

نویسنده

عضو هیآت علمی

چکیده

در این مطالعه، بررسی اثر غلظت‌های مختلف آنتی‌اکسیدان‌های آلفاتوکوفرول، آلبومین سرم گاوی (BSA) و ویتامین C بر شاخص‌های ارزیابی اسپرم بز متعاقب 48 ساعت نگهداری در دمای 5 درجه سانتی‌گراد بررسی شد. تیمارها شامل 7 گروه رقیق‌کننده حاوی آلفاتوکوفرول با دو غلظت 5 و 10 واحد، BSA با دو غلظت 4 و 8 میلی‌گرم در میلی‌لیتر، اسید آسکوربیک با غلظت‌های 3 و 6 میلی‌گرم در میلی‌لیتر و گروه شاهد بدون افزودن ماده آنتی‌اکسیدانی بودند. در زمان های صفر، 24 و 48 ساعت، تحرک کلی و پیشرونده و نیز سلامت غشای اسپرم‌ها ارزیابی شدند. تحرک اسپرم و سلامت غشاء هنگام استفاده از رقیق‌کننده BSA با غلظت 4 میلی‌گرم و ویتامین E با غلظت پنج واحد بیشتر از سایر رقیق‌کننده‌ها بود (05/0P<). اگرچه غلظت‌های بالاتر این 2 آنتی‌اکسیدان باعث افزایش کمتر شاخص‌های مورد ارزیابی شد، اما نسبت به گروه شاهد بهبود نسبی را نشان دادند (05/0P<). غلظت‌های مختلف اسید آسکوربیک بر هیچ‌یک از فراسنجه‌های ارزیابی اسپرم اثر نداشت. براساس نتایج تحقیق حاضر، افزودن BSA و ویتامین E به اسپرم بز در طی فرآیند نگهداری در دمای 5 درجه تحرک کلی و پیشرونده و نیز سلامت غشاء در طی 48 ساعت را بهبود می‌بخشد.

کلیدواژه‌ها


عنوان مقاله [English]

Protective effects of α-tocopherol, BSA and vitamin C on caprine sperm quality during incubation at 5° C

نویسنده [English]

  • Arash Kheradmand
چکیده [English]

This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin C, and control group without any antioxidant. Total sperm motility, progressive motility and HOS test were assessed at h 0, 24 and 48. Using 4 mg of BSA and 5 IU of vitamin E increased sperm motility and plasma membrane integrity compared to other antioxidants (P<0.05). Although, higher concentrations of these antioxidants relatively enhanced sperm quality parameters, however, it was still greater than the control group (P<0.05). Different concentrations of vitamin C did not affect sperm quality parameters. According to the results of the present study, addition of BSA and vitamin E to buck semen improved total and progressive sperm motility as well as plasma membrane integrity during 48 h after incubation at 5 °C.

کلیدواژه‌ها [English]

  • Antioxidant
  • Buck
  • Lipid Peroxidation
  • Membrane integrity
  • Sperm
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