Document Type : Research Paper
Author
Abstract
This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin C, and control group without any antioxidant. Total sperm motility, progressive motility and HOS test were assessed at h 0, 24 and 48. Using 4 mg of BSA and 5 IU of vitamin E increased sperm motility and plasma membrane integrity compared to other antioxidants (P<0.05). Although, higher concentrations of these antioxidants relatively enhanced sperm quality parameters, however, it was still greater than the control group (P<0.05). Different concentrations of vitamin C did not affect sperm quality parameters. According to the results of the present study, addition of BSA and vitamin E to buck semen improved total and progressive sperm motility as well as plasma membrane integrity during 48 h after incubation at 5 °C.
Keywords
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