نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری اصلاح نژاد دام دانشگاه تربیت مدرس
2 عضو هیئت علمی دانشگاه تربیت مدرس
چکیده
در این تحقیق به منظور شناسایی جایگاه‌ها و ژن‌های مرتبط با صفات کیفیت گوشت، مطالعه پویش کل ژنوم (GWAS) با استفاده از یک تراشه SNP ژنوم مرغ (SNP Beadchip (Illumnia 60k Chicken در یک جمعیت F2 حاصل از تلاقی دوطرفه مرغ بومی آذربایجان غربی و لاین B سویه گوشتی آرین انجام شد. برای هر پرنده، صفات شامل: ظرفیت نگهداری آب، رنگ گوشت (روشنایی ، قرمزی و زردی)، میزان نیروی برشی و PH نهایی گوشت اندازه‌گیری شد. با استفاده از دو مدل خطی معمول (GLM) و مدل خطی مختلط فشرده (Compressed MLM) ارتباط هر یک SNPها با صفات کیفیت گوشت بررسی شد. در مجموع تعداد 36 نشانگر SNP در سطح احتمال پیشنهادی و معنی‌داری برای صفات کیفیت گوشت شناسایی شد که تعداد 3 نشانگر SNP با روش Compressed MLM برای صفت فاکتور زردی گوشت و تعداد 18 نشانگر SNP با روش GLM برای صفات فاکتور زردی گوشت، pH نهایی گوشت، ظرفیت نگهداری آب و میزان نیروی برشی معنی‌دار شدند. ژن‌های کاندیدای شناسایی‌شده، عملکرد مولکولی مرتبط با صفات کیفیت گوشت داشتند و لذا می‌توان از این ژن‌های کاندیدا در برنامه‌های اصلاحی طیور مورد استفاده قرار‌داد.
کلیدواژه‌ها
عنوان مقاله [English]
Genome-Wide Association Study for meat quality traits in an F2 intercross between Azerbaijan native chickens and Ariyan broiler line
نویسندگان [English]
- ali javanrouh aliabad 1
- Ali akbar masoudi 2
- alireza ehsani 2
2 member of science Tarbiat Modares University
چکیده [English]
In order to identify loci and genes associated with meat quality traits, genome-wide association study (GWAS) were conducted in a F2 population derived from a reciprocal cross between Azerbaijan native chickens and Aryan broiler line by using Illumnia 60 K Chicken SNP Bead chip. For each bird, a total 6 traits including water holding capacity, meat color lightness (L*), redness (a*), yellowness (b*), shear force and ultimate pH were measured. The SNPs that were associated with meat quality traits were identified using both GLM and compressed mixed linear models (CMLM). A total of 36 SNPs were associated with meat quality traits in the genome–wide significance and suggestive levels, that 3 SNPs were significantly associated with meat color yellowness through CMLM model and 18 SNPs were suggestively associated with meat color yellowness, ultimate pH, water holding capacity and shear force through GLM model. The identified candidate genes have molecular functions related to meat quality traits. So, these candidate genes can be applied in the chicken breeding scheme.
کلیدواژه‌ها [English]
- candidate genes
- chicken
- F2 population
- GWAS
- Meat quality traits
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