Bizhan Mahmoudi; Hedayatollah Roshanfekr; Mohsen Sari; Mohammad Reza Bakhtiarizadeh
Abstract
The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminaltissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using theHiseq2500 illumine ...
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The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminaltissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using theHiseq2500 illumine platform. Hisat2 software was used to align reads to the bovine reference genome and StringTie software package was used toassemble read files into transcripts. Using next generation sequencing, 1636 genes belonging to known intergenic lncRNAs were identified, of which56 genes showed significant differential expression (P≤0.05). Neighbor genes of known intergenic lncRNAs were determined on bovine genome.Analysis of biological pathways and molecular function showed that five biological pathways were significantly (P≤0.05) enriched. These pathwayswere Apelin signaling pathway, Gap junction, Glucagon signaling pathway, Renin secretion, and AGE-RAGE signaling pathway. Moreover, twomolecular functions including gap junction channel activity, and phosphatidyl inositol phospholipase C activity were significantly (P≤0.05) enriched.Some lncRNAs have different expression in healthy and acidosis samples, and the decreased pH acts as a stimulus to activate some biologicalsignaling pathways. In conclusion, it was indicated that lncRNAs with differential expression between the control group and the group affected byacidosis are associated with pathways related to rumen energy metabolism and signaling. Identified differentially expressed lncRNAs could be used asprognostic in acidosis and biomarkers or promising candidates in animal breeding.
elahe sanjari; mahdiyeh zieaadini; Masood asadi fozi; Ali Esmailizadeh koshkoiyeh
Abstract
This wok was conducted to identify polymorphisms in the region of the exon 1 in 5'UTR of myostatin gene and their relationships with the growth traitsin Raeini cashmere goats. Myostatin gene has been identified as a factor causing a phenotype known as double-muscling in which a series of mutations render ...
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This wok was conducted to identify polymorphisms in the region of the exon 1 in 5'UTR of myostatin gene and their relationships with the growth traitsin Raeini cashmere goats. Myostatin gene has been identified as a factor causing a phenotype known as double-muscling in which a series of mutations render the gen inactivity; therefore, able to increase muscle growth. Blood samples were randomly taken from 150 Raeini cashmere goats. Total genomic DNA was extracted and a 701 bp fragment was amplified from the exon 1 of myostatin gene using PCR-RFLP method. The analysis of band patterns indicated three genotypes including AA, AB and BB with frequencies of 0.56, 0.33 and 0.11, respectively. The A and Balleles had a frequency of 0.72 and 0.28, respectively. The analysis of additive and dominance gene actions revealed that the effect of the myostatin gene on growth traits and body size of Raeini cashmere goats is via the dominance mechanism. The results showed that all growth traits except weaning weight were significantly associated with the identified polymorphisms, thus this gene can be used as a molecular marker in Raeini cashmere goats breeding.
Seyed Hamid Mousavi Esfiokhi; Mohammad Reza Sahlabadi; Behzad Khorrami
Abstract
In order to investigate the chemical and nutritional characteristics of pea pod silage (PPS) and its effect on the performance of fattening lambs, 24 Zandi male lambs (initial BW, 33.5 ±1.5 kg) were used in a completely randomized design over 75 days. Experimental treatments were: 1) control; ...
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In order to investigate the chemical and nutritional characteristics of pea pod silage (PPS) and its effect on the performance of fattening lambs, 24 Zandi male lambs (initial BW, 33.5 ±1.5 kg) were used in a completely randomized design over 75 days. Experimental treatments were: 1) control; (basal diet) 2- pea pod silage (PPS replaced wheat straw and alfalfa in the basal diet). The amount of dry matter (DM), crude protein, ether extract, ash, neutral detergent fiber and acid detergent fiber of PPS were determined 20, 11.8, 1.8, 8, 62 and 20.2 percent, respectively, and pH was 6.02. The rumen degradability potential of DM and in vitro gas production potential of PPS after 96 h of incubation were estimated 83 and 58.6 percent, respectively. The use of PPS in fattening lambs rations improved the final body weight and increased the daily weight gain (P<0.05). The amount of DM digestibility was affected by the treatment (P<0.05), but digestibility of other nutrients was not affected by the experimental treatment. Blood parameters (cholesterol, triglyceride, HDL and LDL) were not affected by the experimental treatments, except for the glucose, which significantly decreased by feeding PPS (P<0.05). The experimental treatments had no significant effect on carcass traits. Based on the results of this study, the use of pea pod silage as a cost-effective alternative forage in replacement with common forages such as wheat straw and alfalfa can improve the performance of fattening lambs.
Mohammad Hossein Nemati; Seyed Abdoallah Hosseini; Ali Mansori; Seyed Saeid Mousavi
Abstract
This study was conducted to rank the broiler farms based on buildings, installations and equipment and its effect on production factors. To determine the contribution of each factor in ranking of farms, a multi-criteria decision analysis (SAW) was used. Data of 108 broilers were recorded in specific ...
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This study was conducted to rank the broiler farms based on buildings, installations and equipment and its effect on production factors. To determine the contribution of each factor in ranking of farms, a multi-criteria decision analysis (SAW) was used. Data of 108 broilers were recorded in specific forms and the amount of investment per broiler was calculated for individual factors. To calculate the adjusted weights of parameters, 20 experts were consulted as was specified in forms. The results showed that ventilation (fans and air inlets), roof and wall insulation in a poultry houses have represented about 55 percent of technological factors. The breeding units holding rank 1 and 2 enjoyed high degree of mechanization. As the level of mechanization was improved, the density of chickens per unit area was also increased; These units used more tunnel ventilation and large fans, and their ceiling were insulated using fiberglass, polystyrene, corrugated plastic and thickness of walls were also 35 cm. Mostly external heaters have been installed. Feeding and drinker systems have exerted no important effect on production. Slaughter weight and survival percent were not affected by the degree of mechanization but feed intake and feed conversion ratio decreased and production index increased (p<0.01) in the breeding units holding rank 1 and 2. In general in poultry house, ventilation system and ceiling and wall insulation improved energy efficiency and increased the efficiency of the system.
Mohammad hadi Khabbazan; Abdollah Rezagholivand Lahrud; Saeed mokhtarzadeh; Hojatollah Moosapour
Abstract
The objective of this study was to compare growth performance, birth and weaning body weights, and survivability between purebred Balouchi (n=53) and crossbred Balouchi×Romanov (n=114) lambs. In addition, carcass characteristics (n=24) were determined. Balouchi ewes were inseminated with Romanov ...
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The objective of this study was to compare growth performance, birth and weaning body weights, and survivability between purebred Balouchi (n=53) and crossbred Balouchi×Romanov (n=114) lambs. In addition, carcass characteristics (n=24) were determined. Balouchi ewes were inseminated with Romanov semen to produce crossbred lambs. All experimental lambs were weaned at 77 d of age and slaughtered at 10 months of age. Birth body weight of the purebred Balouchi lambs was significantly greater than for the crossbred lambs (3.75 vs. 3.56 kg, P<0.01). However, weaning body weight and average daily gain from birth until weaning and slaughter were greater (P<0.05) for the crossbred lambs than for the purebred lambs. Slaughter body weight at 10-month age were not different brtween the tow groups (P=0.08). Litter size was also increased by crossbreeding and using hormone (1.13 vs. 1.00), but lamb survivability was not different between the two groups. The fat yield decreased with crossbreeding. Crossbreeding increased (P<0.05) the weights of testis, liver, heart, and lung. It is concluded that crossbreeding Balouchi ewes with Romanov semen resulted in improved litter size, weaning and slaughter body weights, average daily gains from birth until weaning and slaughter, and carcass characteristics without affecting lamb survivability. Therefore, crossbreeding can be a profitable strategy to increase lamb (meat) production in Balouchi sheep.
Hossein Mohammadi; amir hossein khaltabadi farahani
Abstract
Objective: Over the last decade, interest in detection of genes or genomic regions that are targeted by selection has been growing. Identifying signatures of selection can provide valuable insights about the genes or genomic regions that are or have been under selection pressure, which in turn leads ...
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Objective: Over the last decade, interest in detection of genes or genomic regions that are targeted by selection has been growing. Identifying signatures of selection can provide valuable insights about the genes or genomic regions that are or have been under selection pressure, which in turn leads to a better understanding of genotype-phenotype relationships. The aim of this study was to identify candidate genes and genomic region related to litter size trait in Murcia-Granadina goats using the selective sweep methods.
Material and Methods: In this study, data from 643 Murcia-Granadina goat genotyped using Caprine 50 K BeadChip were used. Quality control measures were performed in Plink by setting animal call rate of 0.90, SNP call rate of 0.90 and SNPs with minor allele frequencies (MAF) lower than 0.01. To identify the signatures of selection, two statistical methods of FST and hapFLK were used. Candidate genes were identified by SNPs located at 1% upper range of FST and hapFLK. Finally, GeneCards and UniProtKB databases were also used to interpret the function of the obtained genes.
Results and Discussion: Using FST approach, we identified nine genomic regions on chromosomes 4, 5, 6, 8, 12, 13, 14, 15, and 22 chromosomes. The identified candidate genes associated with litter size trait in these genomic regions included KMT2E, CAMK2D, CTNNAL, DACH1, DNMT3B, STK3. Some of the genes located in identified regions under selection were associated with the oocyte growth, development and differentiation of ovarian follicles, fertility and growth and development of granulosa cells, which can be directly and indirectly related to the trait of the litter size. Also, survey on extracted QTLs was shown that these QTLs in cow orthologous associated sperm count and calf size. The results of hapFLK statistics in this research led to the identification of four genomic regions on chromosomes 1, 2, 5, and 11. The identified candidate genes associated with the litter size trait in these genomic regions included EDA2R, KCNH7 and CNOT11. It was determined that they had different functions in folliculogenesis and spermatogenesis. Also, survey on extracted QTLs was shown in cow orthologous associated calving interval.
Conclusion: By the way, various genes that were founded within these regions can be considered as candidates under selection based on function. However, will be necessary to carry out more association and functional studies to demonstrate the implication of genes obtained from association analyses. Finally, the results of our research can be used to understand the genetic mechanism controlling litter size trait.
Kobra Karimi; Mohammad taghi Beigi nassiri; Mahmood Nazari; khalil Mirzadeh
Abstract
The present study was conducted to evaluate the effect of Tribulus Tresstrise (TT) herb on sex ratio of semen in Arabic Khouzestan ram using real time-qPCR technique using 18 rams in a completely randomized design with 3 treatments. The SRY and PLP genes were amplified to isolate the specific ...
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The present study was conducted to evaluate the effect of Tribulus Tresstrise (TT) herb on sex ratio of semen in Arabic Khouzestan ram using real time-qPCR technique using 18 rams in a completely randomized design with 3 treatments. The SRY and PLP genes were amplified to isolate the specific fragments of Y- and X- chromosome sequences. The treatments included: i) the control group (0% TT), ii) Diet containing 15 g/kg TT, iii) Diet containing 30 g/kg TT. Sperm sampling was taken from all rams at 10 month of age and blood sampling was performed at 8 and 10 month of age. The results showed that expression rate of SRY gene increased with increasing TT level and rams that received 30 g/kg TT diet had the highest SRY gene expression and PLP gene expression decreased with increasing TT level (p-value =0.004). There was positive correlation between Testosterone concentration and SRY gene expression at 8 (0.65) and 10 (0.59) month of age, and the relationship between PLP gene expression and Testosterone concentration was negative and -0.61 and -0.66 at 8 and 10 month of age, respectively (p-value= 0.006). The results indicated that adding Tribulus Tresstrise herb to the ram diet increases the SRY gene expression and also sperm containing Y chromosome. In other words, it increases the sex ratio toward male gens in Arabic Khozestan ram by increasing the androgen hormones.
Noorolahe SHahroodi; Mohammad Rokouei; Hadi Faraji- Arough; Ali Maghsoudi; Morteza Kykha Saber
Abstract
The purpose of this study was to fit different nonlinear models to describe growth curve and selection the best model to describe a growth curve for calves of Sistani calves. Body weight records of 241 calves (118 males and 123 females) collected by the Sistani Dairy Cattle Research Station of Zahak ...
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The purpose of this study was to fit different nonlinear models to describe growth curve and selection the best model to describe a growth curve for calves of Sistani calves. Body weight records of 241 calves (118 males and 123 females) collected by the Sistani Dairy Cattle Research Station of Zahak from year 2010 to 2017 were used. Four nonlinear models (Gompertz, Logistic, Richards, and Weibull) were fitted to the body weight records and the best model was selected by the goodness-of-fit criteria (Mean square error, Bayesian information criterion, Akaike information criterion and corrected coefficient of determination). According to goodness-of-fit criteria, Richards model was the most appropriate model to describe the growth curve in male and female calves. The effect of sex on curve parameters was significant in many functions (P <0.05). Logistic and Richards models had the highest and the lowest initial weight parameter, respectively. Male calves reached to the inflection point in a higher age and weight compared to female calves. According to the results of this study, a proper model can be used to study the growth pattern of this breed in order to better nutritional management and selection for rapid growth with high accuracy.
mehdi Vafaye valleh; nahid Karimi zandi; Farzaneh Bazmandegan Shomeyli
Abstract
In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross ...
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In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross 308 eggs were randomly assigned into two equal experimental groups including test (In ovo injection of Khazak yolk) and control (In ovo injection of Ross yolk) group. After hatching, chickens were reared for six weeks under the same standard environmental conditions with exposure to some certain inflammatory stimuli between 21-28 days of age. Chicken’s blood and/or tissues samples were collected on days 10 and 42, and the samples were analyzed for the target traits. Results showed that Khazak yolk component caused a reduction in the levels of pro-inflammatory TNF-α cytokine in both offspring's liver and intestinal tissue (P<0.05). Compared to the control group, khazak yolk injection was found to enhance the titer of IgA natural antibodyas well as primary and secondary antibody titer response against sheep red blood cells (SRBC) and reduces both serum levels of offspring's CRP protein and liver ALT enzyme (P<0.05). According to the results of the present study, injection of Khazak native hen egg-yolk into the yolk of eggs from Ross 308 commercial broiler breeder can effectively suppress the expression of TNF-α inflammatory cytokine in the offspring's liver and intestinal tissue.
Behrouz Mohammad Nazari; Ardeshir Nejati Javaremi; Mohammad Moradi Shahre Babak; Rostam AbdolahiArpanahi
Abstract
In order to evaluate the effect of genotype by environment interaction on production traits of Holstein cattle of Iran, first lactation test day records of 344170, 135000 and 156840 of milk, fat and protein yield on 34417, 13500 and 15684 cows and SNP markers of 1935 genotyped bulls were used. The ...
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In order to evaluate the effect of genotype by environment interaction on production traits of Holstein cattle of Iran, first lactation test day records of 344170, 135000 and 156840 of milk, fat and protein yield on 34417, 13500 and 15684 cows and SNP markers of 1935 genotyped bulls were used. The production data were retrieved from the Animal Breeding Center and Productions Improvement of Iran’s database which were collected from 2013 to 2018. To consider the interaction of genotype and environment, mean of temperature-humidity index (THI) in three days before each test day records as continuous environmental effect were retrieved from the 35 closest meteorological stations in the vicinity of 139 Holstein herds from 13 provinces. Variance and covariance components were estimated through a single-trait random regression model with orthogonal Legendre polynomials of second order for days in milk and THI using AIREMLF90 software. The results showed that changes in THI across lactation led tofluctuations in additive genetic variance over time. The change in heritability of milk production traits over lactation followed the same trend as additive genetic variance. The results from cross-validation analysis showed that including genomic information into the predictive model, increased prediction accuracy and including THI information increased unbiasedness. Due to the changes in milk production of daughters of bulls across days and THI , genotype by environment interaction should be considered when selecting bulls under different conditions.
efat nasresfahani
Abstract
The objective of this study was to estimate and compare the sensitivity of economic efficiency to change in productive and reproductive performance as well as involuntary culling rate of dairy herds. For this purpose, A bio- economic model developed in DairyVIP software was used to analysis the registered ...
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The objective of this study was to estimate and compare the sensitivity of economic efficiency to change in productive and reproductive performance as well as involuntary culling rate of dairy herds. For this purpose, A bio- economic model developed in DairyVIP software was used to analysis the registered information’s in the annual performance of dairy farms covered by Isfahan Vahdat cooperation. The net annual profit obtained 23310 × 1000 IRR when the average annual of milk yield, pregnancy rate and annual involuntary culling rate were 11588 kg, %18.6 and %18.1, respectively. The annual profit varied by 1353 × 1000 IRR per 1 kg change in the daily milk yield. While for every 1 percent change in the conception and involuntary culling rate, the annual profit varied by 564 and 321 × 1000 IRR, respectively. The breakeven of daily milk yield and pregnancy rate was too changeable to stay the annual interest, So that an increase in daily milk yield by 5 kg could tolerate a decrease in pregnancy rates up to % 8.1. Whereas pregnancy rate should be increased as much as 26.6% of basic conditions to compensate the economic losses due to a decline in daily milk yield by 5kg. Our finding can help the producers to identify the economic value of important traits in cattle annual profitability and take the optimal management decisions to improve economic benefit.
Nejat Badbarin; nymat hydaeat
Abstract
The aim of this study was to map body weight QTLs with some microsatellite markers on chromosome 2 of Markhoz goats. For this purpose a total of 255 offspring including 129 male and 126 female from 8 half-sib sire families were genotyped for 6 microsatellite markers on chromosomes 2. Quantitative traits ...
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The aim of this study was to map body weight QTLs with some microsatellite markers on chromosome 2 of Markhoz goats. For this purpose a total of 255 offspring including 129 male and 126 female from 8 half-sib sire families were genotyped for 6 microsatellite markers on chromosomes 2. Quantitative traits were included weights at birth, 4 months, 6 months, 9 months and 12 months of age. Significant QTL effect was tested using the least squared regression approach using GridQTL (V3.3.0) software. In this study, one putative QTL were detected for weaning weight at 153 cM on chromosome 2 (P<0.01). QTL effect was 4.99 in units of residual standard deviation. The position of this QTL was between the IDVGA64 and OarFCB011 and its distance was measured 10 cM of IDVGA64 markers. In the present study any pleiotropic effect of this QTL with other traits were not recognized. It is expected that future genome wide scan with larger individual per family will help identify pleotropic effects and other QTLs associated with these traits and will provide useful markers for marker-assisted selection of such important traits. According to ensure high confidence interval (CM = 25 cM) Offer is not valid candidate genes for QTL set and is recommended for accurate determination of the QTL in future studies of more offspring in the family are applied.
Masoud Norozi-ِDeeyarjan; ali assadi-alamouti; Ahmad Afzalzadeh; mohsen danesh mesgaran
Abstract
This study examined the effects of partial replacement of soybean meal (SBM) with heat-treated soybean meal (HSBM) in heat-stressed cows. 32 multiparous Holstein cows (DIM = 110 ± 20; milk yield = 40 ± 4.1 kg) were used in a complete block randomized design with a 2×2 factorial arrangment ...
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This study examined the effects of partial replacement of soybean meal (SBM) with heat-treated soybean meal (HSBM) in heat-stressed cows. 32 multiparous Holstein cows (DIM = 110 ± 20; milk yield = 40 ± 4.1 kg) were used in a complete block randomized design with a 2×2 factorial arrangment of treatments. Treatments included two dietary crude protein levels (i.e. 17 and 15.5%) each combined with HSBM partially replaced for SBM. The experiment was conducted in two periods of 28 days with the last seven days for sampling and collection of data of tempreature, nutrient intake and digestibility and blood variables. Average THI showed that cows were in mild heat stress (70.60 and 71.50 for periods 1 and 2, respectively)(p > 0.05). Diets with 15.5% CP and based on HSBM increased intakes of dry and organic matter (p < 0.05); however, this was not observed in diets with 17% CP. Milk yiled and composition, apparent nutrient digestibility and blood variables were unaffected (p > 0.05). Butyric and isovaleric acid were increased in cows received HSBM (p < 0.05); however, level of CP had no effect. Results showed that feeding HSBM in diets with 15.5% CP could be regarded as a nutritional sterategy for improving DM intake of mild heat-stressed cows.
Parvaneh Hashemi; Leila Taherabadi; Farokh Kafilzadeh
Abstract
Introduction: Ensilage is one of the methods of preserving forage plants for livestock feeding. The use of carbohydrate sources to prepare of silage improve the quality of fermentation by producing high lactic acid concentrations. The aerobic stability of silage after exposure to air is one of the quality ...
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Introduction: Ensilage is one of the methods of preserving forage plants for livestock feeding. The use of carbohydrate sources to prepare of silage improve the quality of fermentation by producing high lactic acid concentrations. The aerobic stability of silage after exposure to air is one of the quality parameters of silages. Heterofermentative lactic acid bacteria are among the additives that have been used to improve the aerobic stability of silages. However, there is no information on the use of the Lactobacillus fermentum on the aerobic stability of Napier grass silage with or without the use of carbohydrate sources.
Aim of study: This research was conducted to study the effect of Lactobacillus fermentum 92069 and molasses on fermentation properties, aerobic stability and in vitro digestibility of Napier grass silage as a new forage source (introduced for the first time) in the country.
Material and methods: Napier grass was cultivated at experimental station of school of Agriculture, Razi University on May 2021. In order to prepare Napier grass silage, the forage was harvested, chopped and treated with 0, 3 or 6 % molasses with or without 0, 1 × 106 cfu or 2 ×106 cfu of Lactobacillus Fermentum 92069 per gram of fresh forage. Forage was ensiled in laboratory silos with four replicates. After 90 days of ensiling, chemical composition and fermentation products of silages such as lactic acid, butyric acid, acetic acid, ammonia nitrogen and in vitro digestibility were determined. The fungal population of silages including yeast and mold were also determined. During the aerobic fermentation process, aerobic stability of the silages and changes in pH and the population of fungal in the silages were determined.
Results and Discussion: Increasing the level of molasses was associated with an increase in dry matter and soluble carbohydrates of silage. The lowest pH values (3.90 to 3.97) and higher production of lactic acid concentration (47.9 to 53.3 g/kg dry matter) were observed in silages containing the high level of molasses with or without bacterial inoculant. The effect of using Lactobacillus fermentum increased the production of acetic acid, but had no effect on the aerobic stability. There was no difference in the fungi population of silages from different treatments. The digestibility of dry matter and organic matter and also metabolize energy were higher in silages containing molasses with or without bacterial inoculant.
Conclusion: The current research regarding the Napier grass silage prepared with no additive had a relatively good quality. However, the use of molasses led to an improvement in the anaerobic fermentation and digestibility. The aerobic stability of Napier grass silages in spite of an increase in acetic acid was not affected by the addition of the Lactobacillus fermentum up to 2 ×106 cfu/ g fresh forage.
Mahnaz Salehi; Raziyeh Hezareh Moghadam
Volume 13, Issue 1 , September 2011, , Pages 17-22
Abstract
To study the fleece characteristics of native goats in Sistan and Balouchestan province, fiber sample was taken from 270 females between one to three years old from Khash, Saravan and Iranshahr cities. The differences between age groups for all fleece parameters except fleece weight were not significant. ...
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To study the fleece characteristics of native goats in Sistan and Balouchestan province, fiber sample was taken from 270 females between one to three years old from Khash, Saravan and Iranshahr cities. The differences between age groups for all fleece parameters except fleece weight were not significant. The fleece weight of two and three years old goats were 263.4 (± 7.7) and 315 (± 7.8) gr, respectively and were more than the fleece weight of yearling goats 323 (± 7.7) gr. The results of cashmere in Khash goats was highest (26.3 ± 1%), and the goats of Iran-shahr had the finest cashmere of (12.5± 0.3 micron).
Mehdi Abediny Sanigy; farid shariatmadari; Mohammad Amir Karimi Torshizi
Volume 13, Issue 2 , March 2012, , Pages 19-27
Abstract
400 one day-old broiler chicks were allocated on a randomized complete design experiment with five treatments consisting of four replications for 42 days. Experiment treatments were included: 1 . based diet consist 20 percent barley (barley control), 2 . barley control + 0.1 percent of mixed essential ...
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400 one day-old broiler chicks were allocated on a randomized complete design experiment with five treatments consisting of four replications for 42 days. Experiment treatments were included: 1 . based diet consist 20 percent barley (barley control), 2 . barley control + 0.1 percent of mixed essential oil (Antibiofin), 3 . barley control + 0.2 percent organic acid, 4 . barley control + 15 ppm of antibiotic (virginiamycin) and 5 . corn-soybean based diet as corn control. In addition, Basal diet supplemented with commercial enzyme preparation. There was significant difference within treatments for 42 age's body weight and FCR. No significant differences in FI were noted among treatment during experiment. Antibody titer against (SRBC) among groups in secondly immuno response had a significant effect (P< 0.05). The villus height of deudenum and Jejunum was significant differences among the treatment groups. It can be concluded that by using additives such as medicinal plant and organic acids in diets containing barley, can be achieved to similar performance of corn based diets.
karim hasanpur; Ali Asghar Aslaminejad; Mohammad Moradi Shahrbabak
Abstract
The effects of days in milk (DIM) on the curves of milk yield and milk fat percentage in Holstein cows of Iran were studied. A total of 964,606 and 885,346 test day records were used for milk yield and milk fat percentage, respectively. The data were grouped in four classes including: 1) 240 < DIM ...
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The effects of days in milk (DIM) on the curves of milk yield and milk fat percentage in Holstein cows of Iran were studied. A total of 964,606 and 885,346 test day records were used for milk yield and milk fat percentage, respectively. The data were grouped in four classes including: 1) 240 < DIM < 301, 2) 300 < DIM < 401, 3) 400 < DIM < 501 and 4) 500 < DIM < 601 days. To fit the curves, nine non-linear models for every one of the classes were applied. The Narushin & Takma 1 function which previously has been used for fitting the production and growth characteristics of chickens was used in this study. It showed a relatively high goodness of fit in fitting milk yield and milk fat percentage curves compared to other functions. Wood, Dijkstra and Rook functions showed higher Adjusted R2 whereas the third order Legendre polynomial function showed the lowest value in all the classes. The cows had longer lactation period, showed higher persistency and yielded higher daily milk at peak and post peak stages of lactation period. The forth class of cows yielded considerably higher milk and fat (based on 305 day production) compared to other groups. On the contrary, the rate of reduction of milk yield was significantly high for cows located in the lowest lactation length class and the slope of declining stage of milk yield curve was sharper.
Atefeh Berenjian; Seyed Davood Sharifi; Abdollah Mohammadi Sangcheshmeh; Shokoofeh Ghazanfari
Abstract
Effects of chromium nano particles (NanoCr) on performance and meat quality of Japanese quail under physiological stress was determined by using 360 birds in a completely randomized design with six treatments, four replicates and 15 birds per each, during 17- 35 d of age. The tratments were ...
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Effects of chromium nano particles (NanoCr) on performance and meat quality of Japanese quail under physiological stress was determined by using 360 birds in a completely randomized design with six treatments, four replicates and 15 birds per each, during 17- 35 d of age. The tratments were negative control (non-stressed), and five groups under physiological stress which fed on diets supplemented with different levels of NanoCr (0, 200, 400, 800 and 1200 µgkg-1). Dexamethasone (0.6 mgkg-1 BW) was added to diet for induction of physiological stress. Physiological stress reduced feed intake and growth, whereas increased feed conversion ratio (FCR) and thigh and breast muscles malondialdehyde (MDA) concentration (P<0.05). Stressed birds were fed on diets containing increased levels of NanoCr linearly improved weight gain (P< 0.02), and decreaced FCR (P< 0.05), MDA levels in breast (P< 0.04), and thigh muscles (P< 0.006). The results showed that supplementation of diet with NanoCr can alleviate the negative effects of physiological stress on performance and meat quality of quails.
Zohreh Shirmoradi; Abdol Reza Salehi; Rostam Pahlavan; Mohamad Reza Mollasalehi
Volume 12, Issue 2 , October 2010, , Pages 21-28
Abstract
In this study, the genetic and phenotypic trend of milk and fat production traits were estimated form 94121 cows’ first lactation records and that for age at first calving and calving interval traits were estimated from 41900 cows’ reproduction records. Data related to Iranian Holstein dairy ...
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In this study, the genetic and phenotypic trend of milk and fat production traits were estimated form 94121 cows’ first lactation records and that for age at first calving and calving interval traits were estimated from 41900 cows’ reproduction records. Data related to Iranian Holstein dairy cattle from 1995 to 2003. Data were analyzed, using univariate and mulitivariate animal model with genetic group effects. The heritability of milk yield, fat yield and fat percentage, age at first calving and calving interval were 0.29 (±0.01), 0.22 (±0.01), 0.38 (±0.01), 0.055 (±0.01) and 0.034 (±0.005), respectively. The linear regression of average breeding values and phenotypic values of cows for a trait on birth year provided an estimate of genetic and phenotypic trend, respectively. There genetic trend for milk yield, fat yield and Calving Interval were 53.5 kg, 1.07kg and 0.46 day, respectively. Their trend for fat percentage and Age at First Calving were -0.011% and -0.23 day, respectively. There was a positive phenotypic trend for milk yield, fat yield and fat percentage. This trend was negative for Age at First Calving and Calving Interval.
F. Pourbayramian; M. Ghaderzadeh; H. A. Deljoo Isaloo; P. Biabani; M. B. Shams Borhan; P. Barenj Foroush
Abstract
The current study was designed to estimate the frequency of IGF-I gene 1 polymorphism and investigate if their polymorphisms have association with biometric traits in Moghani sheep by PCR-SSCP method. For this purpose, a number of 100 sheep were randomly chosen from Moghani sheep Jafarabad, Moghan breeding ...
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The current study was designed to estimate the frequency of IGF-I gene 1 polymorphism and investigate if their polymorphisms have association with biometric traits in Moghani sheep by PCR-SSCP method. For this purpose, a number of 100 sheep were randomly chosen from Moghani sheep Jafarabad, Moghan breeding station that located in Ardabil state and blood samples were collected from the left jugular vein using vacuum tubes. DNA were extracted from blood samples that using the salting-out procedure and amplified a fragment of 279 bp in size from IGF-I gene exon 1. The Single-Strand Conformation Polymorphism (SSCP) method was used for genotyping. Electrophoresis of PCR products were done on acrylamide gel that observed the polymorphic in this region. Based on SSCP patterns, three genotypes BC, BD and BB obtained with frequencies of 65, 26 and nine percent, respectively. The genotypes in this locus deviated from HWE. The traits data were analyzed using the SAS program. The BB genotype were associated with Leg girth but no association of the other genotypes with the withers height, back height, body length, breast girth other examined biometric traits were found (P< 0.05).
Negin Jamali Emam Gheis; Ali Sadeghisefidmazgi; Mohammad Mehdi Moeini
Abstract
In this study, somatic cell counts (SCC) of milk were determined in six industrial and traditional dairyfarms in Tehran province during different seasons. The prevalence of udder disorders and mastitis wasestimated on the base of SCC as an indicator as well. In this research, industrial and traditional ...
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In this study, somatic cell counts (SCC) of milk were determined in six industrial and traditional dairyfarms in Tehran province during different seasons. The prevalence of udder disorders and mastitis wasestimated on the base of SCC as an indicator as well. In this research, industrial and traditional dairyfarms during 9 months were studied by total 32620 SCC records. Milk samples were collectedindividually. Least square mean (±standard error) (n×1000 per ml) in industrial and traditional culturesystems were 80.11 (±12.60) and 234.57 (±12.97), respectively. The SCC in traditional dairy farms wasapproximately 2.9 times higher than those of industrial ones (P<0.05). The highest SCC was found insummer that was statistically different from spring and autumn (P<0.05). In industrial dairy farms, subclinicaland clinical mastitis were estimated to 36.6 and 11.8%, respectively. The corresponding valuesfor traditional ones were 59.6 and 34.7%, respectively. The results showed that if the SCC decreases by ahalf, mastitis disease incidence would be reduced up to 30-50 percent.
Mohammad Roostaei-Ali Mehr; Marziyeh Ghamgosar; Mahmoud Haghighian Roodsari
Abstract
Experiment was conducted to evaluate the effect of SAF-Mannan on productive performance and intestinal microflora by 180 day-old chicks. At six d, Chicks were divided to five groups (treatments) and each group was split into three replicates which included 12 birds. Amounts of 0.3 mg/Kg lincomycin premix, ...
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Experiment was conducted to evaluate the effect of SAF-Mannan on productive performance and intestinal microflora by 180 day-old chicks. At six d, Chicks were divided to five groups (treatments) and each group was split into three replicates which included 12 birds. Amounts of 0.3 mg/Kg lincomycin premix, 0 (control), 0.5 (A0.5) and 1 (S1) g/Kg SAF-Mannan for total period were added to diet of the first four treatments and also 0.25and 0.75 g/Kg SAF-Mannan for starter and grower, respectively were added to diets of the fifth treatment (S0.25-0.75). Poupulation of E. coli and lactobacillus of ileal samples were assayed at 18, 28 and 38 d of age. The results showed that birds fed on SAF-Manan diets had lower feed conversion ratio compared to control group (P<0.05). At 18 and 28 d, the E. coli count in the al content of SAF-Manan treatments was lower than controland lincomycin treatment (P<0.05). At 28 d, lactobacillus count in ileal content of birds fed on dietd containing 1 g SAF-Manan was higher compared to other treatments (P<0.05). It could be conclude that addition of 0.5 g/Kg SAF-Manan to broiler diet improve weight gain and feed conversion ratio probably, by modifying of intestinal microflora.
Tavakol Akhavan Gigloo; Fardin Hozhabri; Manouchehr Souri
Abstract
A total of 24 male and female Sanjabi suckling lambs with an average weight of 4.2 kg at birth day were used in a 90-day trial period using randomized complete block design for evaluation of two dietary antioxidants. The lambs were received forage and concentrate from the second week of birth in addition ...
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A total of 24 male and female Sanjabi suckling lambs with an average weight of 4.2 kg at birth day were used in a 90-day trial period using randomized complete block design for evaluation of two dietary antioxidants. The lambs were received forage and concentrate from the second week of birth in addition to maternal milk. The experimental diets included control (milk and starter), control + 10% lemon balm (Melissa officinalis) and control + one tablet CoQ10 (30 mg). Every 30 day of experiment blood samples were drawn from jugular vein to measure some hematological and enzymatic parameters. No significant difference was observed among treatments for daily milk, dry matter intake and performance of lambs. Hematocrit was not affected due to lemon balm but it was increased (P<0.05) by CoQ10. Diets containing antioxidants increased the total white blood cells compared to control (P<0.05); but monocytes and basophils did not alter these parameters. Lemon balm reduced the lymphocytes and increased neutrophils while CoQ10 did not influence these two parameters. Eosinophil in blood samples of lambs received Lemon balm or CoQ10 were more than that of control (P<0.05). The concentrations of glutathione peroxidase, immunoglobulin-G and total antioxidant capacity were not affected by treatments, but lemon balm supplementation decreased (P<0.05) malondialdehyde index. The results of this experiment showed, the use of lemon balm in 10% of diet as well as 30 mg/day of coQ10 in suckling lambs’ diet improved immune system, however no significant changes observed in the performance of the lambs.
Hassan Fazaeli; Farshid Mirzaei; Ahmad Afzalzadah; Mohammad Ali Norouzian; Mahdi Nadalian; hadi hosseini
Abstract
This study was conducted to investigate the effect of dry corn gluten feed (DCGF) on nutritive value of corn silage by in vitro and in vivo methods. The firs stage of the experiment was conducted in a completely randomized design, included three treatments and 5 replicates, in which chopped ...
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This study was conducted to investigate the effect of dry corn gluten feed (DCGF) on nutritive value of corn silage by in vitro and in vivo methods. The firs stage of the experiment was conducted in a completely randomized design, included three treatments and 5 replicates, in which chopped corn forage was mixed with 0.0, 5 and 10 percent of DCGF and ensiled in 200L polyethylen barrels. After three months of ensiling, the silages were opened and evaluated. Voluntary intake and digestibility of the silages along with basal diet (Alfalfa hay+wheat straw+barley ground) were determined, using mature (two years old) male Shal sheep. Including of DCGF to corn silage increased (p<0.05)dry matter (DM) and crude protein (CP) in the silages respectively, but pH and organic matter values were not affected by the treatments. However, the DM content did not reach to optimum (35%) point. Concentration of neutral detergent fibre (NDF) was increased but acid detergent fibre (ADF) was decreased (p<0.05) as DCGF was increased in the corn silages. Addition of CGF increased dry matter intake of the silages (p<0.05). The digestibility of DM, OM and NDF were higher in silages containing DCGF (p<0.05). The intake of the digestible DM, OM and NDF were higher in DCGF content silages than those of basal diet and control silage (p<0.05). In general,adding DCGF to corn silage could improve silage quality as well as increasing voluntary intake (g/d) and digestibility when fed to sheep.
Somayeh Jalalinia; Farhang Fatehi; Mehdi Dehghan banadaky; Hossein Rokhforoz
Abstract
To investigate the interaction effects between season and dietary forage particle size on milk yield and composition, one hundred early lactating Holstein were allocated. Dietary forage particle size included long (7.7 mm) and fine (4.4 mm), were adjusted as an experimental treatment per season. The ...
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To investigate the interaction effects between season and dietary forage particle size on milk yield and composition, one hundred early lactating Holstein were allocated. Dietary forage particle size included long (7.7 mm) and fine (4.4 mm), were adjusted as an experimental treatment per season. The results showed that raw milk yield, protein, lactose and total solid percentage of milk were higher within spring compared to summer (p< 0.01). Milk yield and milk lactose percentage for cows fed fine forage particle size trended to be higher than cows fed long forage particle size. Also, the amount of dry matter consumed within two hours after feeding for summer was higher than spring. Totally results revealed that cows fed with fine forage particle size had more homogeneous feeding behavior pattern within both studied season than cows fed long forage particle size.