Association study between some of biometric traits and IGF-I gene exon 1 polymorphism in Moghani sheep

Pourbayramian, F.; Ghaderzadeh, M.; Deljoo Isaloo, H. A.; Biabani, P.; Shams Borhan, M. B.; Barenj Foroush, P.

doi:10.22059/jap.2012.32039

Document Type : Research Paper

Authors

¹ M.Sc. Graduated, Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia - Iran

² – M.Sc. Graduated, Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia - Iran (Corresponding Author *)

³ M.Sc. Graduated, Department of Animal Science, Faculty of Agriculture, Zanjan University, Zanjan - Iran

⁴ M.Sc. Graduated, Department of Animal Science, Faculty of Agriculture, Islamic Azad University Maragheh Branch, Mahabad - Iran

⁵ B.Sc. Student, Department of Animal Science, Faculty of Agriculture, Islamic Azad University Tabriz Branch, Tabriz - Iran

https://doi.org/10.22059/jap.2012.32039

Abstract

The current study was designed to estimate the frequency of IGF-I gene 1 polymorphism and investigate if their polymorphisms have association with biometric traits in Moghani sheep by PCR-SSCP method. For this purpose, a number of 100 sheep were randomly chosen from Moghani sheep Jafarabad, Moghan breeding station that located in Ardabil state and blood samples were collected from the left jugular vein using vacuum tubes. DNA were extracted from blood samples that using the salting-out procedure and amplified a fragment of 279 bp in size from IGF-I gene exon 1. The Single-Strand Conformation Polymorphism (SSCP) method was used for genotyping. Electrophoresis of PCR products were done on acrylamide gel that observed the polymorphic in this region. Based on SSCP patterns, three genotypes BC, BD and BB obtained with frequencies of 65, 26 and nine percent, respectively. The genotypes in this locus deviated from HWE. The traits data were analyzed using the SAS program. The BB genotype were associated with Leg girth but no association of the other genotypes with the withers height, back height, body length, breast girth other examined biometric traits were found (P< 0.05).

Keywords

20.1001.1.20096776.1391.14.2.3.6

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Association study between some of biometric traits and IGF-I gene exon 1 polymorphism in Moghani sheep

References

References

Volume 14, Issue 2 - Serial Number 2January 2012Pages 21-30

Volume 14, Issue 2 - Serial Number 2
January 2012
Pages 21-30