Document Type : Research Paper

Authors

1 Mehdi Vafaye Valleh. Department of Animal Science, Faculty of Agriculture, University of Zabol, P. O. Box 98615–538, Zabol, Iran

2 ندارد

Abstract

In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross 308 eggs were randomly assigned into two equal experimental groups including test (In ovo injection of Khazak yolk) and control (In ovo injection of Ross yolk) group. After hatching, chickens were reared for six weeks under the same standard environmental conditions with exposure to some certain inflammatory stimuli between 21-28 days of age. Chicken’s blood and/or tissues samples were collected on days 10 and 42, and the samples were analyzed for the target traits. Results showed that Khazak yolk component caused a reduction in the levels of pro-inflammatory TNF-α cytokine in both offspring's liver and intestinal tissue (P<0.05). Compared to the control group, khazak yolk injection was found to enhance the titer of IgA natural antibodyas well as primary and secondary antibody titer response against sheep red blood cells (SRBC) and reduces both serum levels of offspring's CRP protein and liver ALT enzyme (P<0.05). According to the results of the present study, injection of Khazak native hen egg-yolk into the yolk of eggs from Ross 308 commercial broiler breeder can effectively suppress the expression of TNF-α inflammatory cytokine in the offspring's liver and intestinal tissue.
 

Keywords

1.         Aviagen, Ross broiler management handbook (2014) Aviagen Group Huntsville, AL.
2.         Cerutti A, Chen K and Chorny A (2011) Immunoglobulin responses at the mucosal interface. Annual Review of Immunology, 29: 273-93.
3.         Chang Q, Wang W, Regev-Yochay G, Lipsitch M and Hanage WP (2015) Antibiotics in agriculture and the risk to human health: how worried should we be? Evolutionary Applications 8(3): 240-7.
4.         Chen J, Tellez G, Richards JD and Escobar J (2015) Identification of Potential Biomarkers for Gut Barrier Failure in Broiler Chickens. Frontiers in Veterinary Science 2: 1-10.
5.            Dou T, Zhao S, Rong H, Gu D, Li Q, Huang Y, Xu Z, Chu X, Tao L, Liu L, Ge C, Pas Te and Jia J (2017) Biological mechanisms discriminating growth rate and adult body weight phenotypes in two Chinese indigenous chicken breeds. BMC Genomics 18(1): 469-481.
6.         Fukui H (2015) Gut-liver axis in liver cirrhosis: How to manage leaky gut and endotoxemia. World Journal of Hepatology, 7(3): 425-42.
7.         Garnier R, Ramos R, Staszewski V, Militao T, Lobato E, Gonzalez-Solis J, Boulinier T (2012) Maternal antibody persistence: a neglected life-history trait with implications from albatross conservation to comparative immunology. Proceedings of the Royal Society B: Biological Sciences 279(1735): 2033-41.
8.         Gentile LF, Cuenca AG, Efron PA, Ang D, Bihorac A and McKinley BA (2012) Persistent inflammation and immunosuppression: a common syndrome and new horizon for surgical intensive care. Journal of Trauma and Acute Care Surgery 72(6): 1491-501.
9.         Giannini E.G, Testa R and Savarino V (2005) Liver enzyme alteration: a guide for clinicians. Canadian Medical Association Journal 172(3): 367-79.
10.      Gomez de Aguero M, Ganal-Vonarburg SC, Fuhrer T, Rupp S, Uchimura Y and Li H (2016) The maternal microbiota drives early postnatal innate immune development. Science 351(6279): 1296-302.
11.      Hamal K. R, Burgess S, Pevzner I, and Erf G (2006) Maternal antibody transfer from dams to their egg yolks, egg whites, and chicks in meat lines of chickens. Poultry science, 85(8):1364-1372.
12.      Jain S, Gautam V and Naseem S (2011) Acute-phase proteins: As diagnostic tool. Journal of Pharmacy and Bioallied Sciences, 3(1): 118-127. 
13.      Koch MA, Reiner GL, Lugo KA, Kreuk LS, Stanbery AG, Ansaldo E (2016) Maternal IgG and IgA Antibodies Dampen Mucosal T Helper Cell Responses in Early Life. Cell 165(4): 827-841.
14.      Levitt DG and Levitt MD (2016) Human serum albumin homeostasis: a new look at the roles of synthesis, catabolism, renal and gastrointestinal excretion, and the clinical value of serum albumin measurements. International Journal of General Medicine 9: 229-55.
15.      Li X, Wang L, Zhen Y, Li S and Xu Y (2015) Chicken egg yolk antibodies (IgY) as non-antibiotic production enhancers for use in swine production: a review. Journal of Animal Science and Biotechnology 6(1): 40-50.
16.      Mahdavi AH, Rahmani HR, Nili N, Samie AH, Soleimanian-Zad S and Jahanian R (2010) Effects of dietary egg yolk antibody powder on growth performance, intestinal Escherichia coli colonization, and immunocompetence of challenged broiler chicks. Poultry Science 89(3): 484-94.
17.      Mashukova A, Wald FA and Salas PJ (2011) Tumor necrosis factor alpha and inflammation disrupt the polarity complex in intestinal epithelial cells by a posttranslational mechanism. Molecular and Cellular Biology 31(4): 756-65.
18.      Mehta M, Ahmed S and Dryden G (2017) Immunopathophysiology of inflammatory bowel disease: how genetics link barrier dysfunction and innate immunity to inflammation. Innate Immunity 23(6): 497-505.
19.      Oesser S, Schulze C and Seifert J (1999) Protective capacity of a IgM/IgA-enriched polyclonal immunoglobulin-G preparation in endotoxemia. Research in Experimental Medicine 198(6): 325-39.
20.      Parnham MJ, (2016) Compendium of Inflammatory Diseases (First Edition). Basel, Switzerland: Springer.
21.      Schat KA, Kaspers B and Kaiser P (2014) Avian Immunology (Second Edition) Boston, Academic Press. Elsevier
22.      Tirawattanawanich C, Chantakru S, Nimitsantiwong W and Tongyai S (2011) The effects of tropical environmental conditions on the stress and immune responses of commercial broilers, Thai indigenous chickens, and crossbred chickens. The Journal of Applied Poultry Research 20(4): 409-420.
23.      Tripathi A, Debelius J, Brenner DA, Karin M, Loomba R and Schnabl B (2018) The gut–liver axis and the intersection with the microbiome. Nature Reviews Gastroenterology & Hepatology 15(7): 397-411.
24.      Van der Most, P. J., de Jong, B., Parmentier, H. K. & Verhulst, S. 2011. Trade-off between growth and immune function: a meta-analysis of selection experiments. Functional Ecology 25: 74-80.
25.      Wondmeneh E, Van Arendonk JA, Van der Waaij EH, Ducro BJ and Parmentier HK (2015) High natural antibody titers of indigenous chickens are related with increased hazard in confinement. Poultry Science. 94(7): 1493-8.