Volume 18, Issue 4 , December 2016, Pages 647-659
Abstract
Bovine mastitis is an inflammation disease of the mammary gland that impose considerable costs to the dairy industry. Regulatory mechanisms of this disease is complex and controlled by various gene regulatory elements and more studies are needed to better understand this disease. In the present study ...
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Bovine mastitis is an inflammation disease of the mammary gland that impose considerable costs to the dairy industry. Regulatory mechanisms of this disease is complex and controlled by various gene regulatory elements and more studies are needed to better understand this disease. In the present study aimed to better understand of regulatory elements involved in mastitis, milk samples of two groups of healthy and infected cows during time series of 0, 12, 24, 36 and 48 hours after contamination were collected. The miRNA-seq data obtained from the milk samples and using the advanced bioinformatics, novel miRNAs, their targets and probability functions, isomirs and novel miRNAs* were identified. The results led to the identification of 92 novel miRNA including 26 miRNAs with homologous and 66 miRNAs without homologous genes in other species. Investigation of the functional groups of predicted targets genes, confirmed the roles of new miRNAs in response to internal and external stimulations, apoptosis and production of immunoglobulin. Furthermore, 135 novel miRNAs were identified. Also, 493 novel isomeric sibling miRNAs (isomers) were discovered that immune related functions of these isomirs were demonstrated in some species like human and mouse. Identification of miRNAs target genes with associated functions in mastitis, including safety, apoptosis and inflammation, can indicated the possible regulatory roles of the identified miRNAs in mastitis.
Nejat Badbarin; nymat hydaeat
Volume 18, Issue 4 , December 2016, Pages 661-670
Abstract
The aim of this study was to map body weight QTLs with some microsatellite markers on chromosome 2 of Markhoz goats. For this purpose a total of 255 offspring including 129 male and 126 female from 8 half-sib sire families were genotyped for 6 microsatellite markers on chromosomes 2. Quantitative traits ...
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The aim of this study was to map body weight QTLs with some microsatellite markers on chromosome 2 of Markhoz goats. For this purpose a total of 255 offspring including 129 male and 126 female from 8 half-sib sire families were genotyped for 6 microsatellite markers on chromosomes 2. Quantitative traits were included weights at birth, 4 months, 6 months, 9 months and 12 months of age. Significant QTL effect was tested using the least squared regression approach using GridQTL (V3.3.0) software. In this study, one putative QTL were detected for weaning weight at 153 cM on chromosome 2 (P<0.01). QTL effect was 4.99 in units of residual standard deviation. The position of this QTL was between the IDVGA64 and OarFCB011 and its distance was measured 10 cM of IDVGA64 markers. In the present study any pleiotropic effect of this QTL with other traits were not recognized. It is expected that future genome wide scan with larger individual per family will help identify pleotropic effects and other QTLs associated with these traits and will provide useful markers for marker-assisted selection of such important traits. According to ensure high confidence interval (CM = 25 cM) Offer is not valid candidate genes for QTL set and is recommended for accurate determination of the QTL in future studies of more offspring in the family are applied.
shoheil mirhabibi; nasser Emam jomeh kashan; shahabodin gharahveysi
Volume 18, Issue 4 , December 2016, Pages 671-678
Abstract
The present investigation was undertaken to study reason of culling, non genetic effects and genetic parameters of longevity with two method linear model and Weibull. A data set of information from Holstein dairy cows of Isfahan province (Iran) was used for this study. The culling date of 36340 animals ...
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The present investigation was undertaken to study reason of culling, non genetic effects and genetic parameters of longevity with two method linear model and Weibull. A data set of information from Holstein dairy cows of Isfahan province (Iran) was used for this study. The culling date of 36340 animals from 65 herds recorded in 1993-2014 was used. From 33219 culled cows in these years were 27.9 and 72.1 percent for voluntary and involuntary culling respectively. Lifespan and Length of productive life (LPL) were analyzed for longevity. Functional productive life (FPL) was estimated by adjusting LPL for first lactation milk yield relative to herd average. The mean of lifespan and length of productive life were estimated to be 954 and 1710 (days) respectively. The results with GLM procedure showed that HYS and age at the first calving had significant on length of productive life. The results with Lifereg procedure with Weibull function showed that effects of herd, year of first calving (p
sajad pezeshki najafabadi; abolfazl shirazi
Volume 18, Issue 4 , December 2016, Pages 679-686
Abstract
Transcription factors have important role in controls of animal fertility by binding to promoter regions of genes and regulation of their expression. In this study, nobox, ovol1 and zp3 genes expression, that are candidate genes involved in regulatory pathways of genes, in two categories of uniparous ...
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Transcription factors have important role in controls of animal fertility by binding to promoter regions of genes and regulation of their expression. In this study, nobox, ovol1 and zp3 genes expression, that are candidate genes involved in regulatory pathways of genes, in two categories of uniparous and multiparous Shall sheep were investigated. According to the pedigree of animals, a totally of six ewes selected from the herd of Qazvin Shall breeding center and applied for this experiment. After estrus synchronization, ewes were operated by general anesthesia technique and ovarian mature follicles were extracted by aspiration. After RNA extraction, sample’s cDNA was constructed. To investigation the expression of the above genes, a set of specific primers was designed for a gene, and then relative expression of these genes were measured using Real-Time PCR method and by employment of ∆∆Ct approach. Comparisons between the averages of data were done by T-Test Statistical procedure. In addition, promoter region and transcription factors of the genes investigated using BDGP software. The expression of nobox and ovol1 genes were significant in multiparous animals category (P
Volume 18, Issue 4 , December 2016, Pages 687-696
Abstract
This research was conducted to study genetic and phenotypic relationships between growth curve parameters in Mehraban sheep. The data set used in this study were obtained from the Agricultural Organization of Hamedan province and comprised 35414 body weight records from birth to 365 days of age during ...
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This research was conducted to study genetic and phenotypic relationships between growth curve parameters in Mehraban sheep. The data set used in this study were obtained from the Agricultural Organization of Hamedan province and comprised 35414 body weight records from birth to 365 days of age during 2001to 2011. Parameters of Brody growth model were studied which included A (asymptotic mature weight or average mature weight of animal), B (proportion of asymptotic mature weight to weight gain as a percent of mature weight) and K (growth rate until reaching to mature weight). Fixed factors affecting on growth curve parameters were identified using the GLM procedure of SAS software. The model of genetic analysis included direct genetic effect, maternal genetic effect, maternal permanent environmental effect and covariance between direct genetic and maternal genetic effects. To estimate genetic relationship between growth curve parameters, Bayesian method based on Gibbs sampling was used. Direct heritabilities for growth curve parameters of A, B and K were 0.29, 0.63 and 0.27, respectively. Direct genetic correlation between A and B was positive and high (0.58), which indicates that these traits are aligned with each other while the correlations between B and K (-0.69) and between A and K (-0.54) were negative. The results indicated that improvement of growth curve parameters of Mehraban sheep is possible in selection programs. Therefore, it is worthwhile to propose an optimum selection strategy for obtaining appropriate growth curve in Mehraban sheep through changing genetically the model parameters.
ali javanrouh aliabad; Ali akbar masoudi; alireza ehsani
Volume 18, Issue 4 , December 2016, Pages 697-709
Abstract
In order to identify loci and genes associated with meat quality traits, genome-wide association study (GWAS) were conducted in a F2 population derived from a reciprocal cross between Azerbaijan native chickens and Aryan broiler line by using Illumnia 60 K Chicken SNP Bead chip. For each bird, a total ...
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In order to identify loci and genes associated with meat quality traits, genome-wide association study (GWAS) were conducted in a F2 population derived from a reciprocal cross between Azerbaijan native chickens and Aryan broiler line by using Illumnia 60 K Chicken SNP Bead chip. For each bird, a total 6 traits including water holding capacity, meat color lightness (L*), redness (a*), yellowness (b*), shear force and ultimate pH were measured. The SNPs that were associated with meat quality traits were identified using both GLM and compressed mixed linear models (CMLM). A total of 36 SNPs were associated with meat quality traits in the genome–wide significance and suggestive levels, that 3 SNPs were significantly associated with meat color yellowness through CMLM model and 18 SNPs were suggestively associated with meat color yellowness, ultimate pH, water holding capacity and shear force through GLM model. The identified candidate genes have molecular functions related to meat quality traits. So, these candidate genes can be applied in the chicken breeding scheme.
Volume 18, Issue 4 , December 2016, Pages 711-718
Abstract
A total of 96 semen samples were collected from Sanjabi breed sheep over two years and during autumn and spring seasons in order to investigate the leptin gene polymorphism and its relationship with the characteristics of sperm quality and testicular size. At the same time, the dimensions of length, ...
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A total of 96 semen samples were collected from Sanjabi breed sheep over two years and during autumn and spring seasons in order to investigate the leptin gene polymorphism and its relationship with the characteristics of sperm quality and testicular size. At the same time, the dimensions of length, width and scrotal circumference were measured and blood samples were taken from sheep jugular vein for extract DNA. PCR was performed using specific primers to amplify a 463bp fragment including exon 3 of leptin gene. After PCR and determining three GG, GT and TT genotypes in the studied samples, it was determined that leptin gene polymorphism had significant relation with individual motility (P<0.05) and motility spermatozoa in animals with GG genotype had the highest. sperm volume, sperm concentration, total sperm ejaculated, hypo osmotic swelling test (HOS), viability and sperm index, in animals with GG genotype had the highest (P<0.05). In animals with TT genotype mean of length and scrotal circumference and in GT genotypes also testicular width and volume were more compared to GG genotypes. These results showed of this leptin gene polymorphism in order to improving fertility in breeding programs of Sanjabi sheep had high heterozygosity, it seems that more studies in larger populations are needed to better understand the relation of other leptin gene polymorphisms on ram fertility.
Morteza Chaji
Volume 18, Issue 4 , December 2016, Pages 719-728
Abstract
This experiment was conducted to determine chemical composition, and fermentation and digestion characteristics of black mulberry leaves, or diets content 0, 25, 50, 75 and 100% of it, by whole rumen microorganisms or rumen fungi. Dry matter, crude protein, NDF, ADF, Ash and tannin of the black mulberry ...
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This experiment was conducted to determine chemical composition, and fermentation and digestion characteristics of black mulberry leaves, or diets content 0, 25, 50, 75 and 100% of it, by whole rumen microorganisms or rumen fungi. Dry matter, crude protein, NDF, ADF, Ash and tannin of the black mulberry leaves was 96.5, 19.30, 36.82, 15.90, 9.31 and 0.30%, respectively. Partitioning factor, truly organic matter degradability, microbial biomass and microbial biomass efficiency in the black mulberry leaves were more than alfalfa hay (P<0.05). The gas production was increased by increasing amount of the black mulberry leaves, so that diet contains 30% of the black mulberry leaves had the hiegest gas production (P<0.05). The organic matter digestibility, cell wall degradability, metabolizable energy and short chain fatty acids of experimental diets increased by increasing the amount of the black mulberry leaves in diet (P<0.05). Therefore, the replacement of the alfalfa hay with black mulberry leaves in diet, has no negative effect on rumen microorganisms, especially isolated rumen fungi which are the digester of fiber and harsh parts of plants, and due to the higher protein and lower fiber contents of the black mulberry leaves, may be replaced it instead of alfalfa hay in fattening lamb diets.
Mohammad Ebrahim Nooriyan Soroor; Mohammad Ebarhim Nooriyan Soroor; Mohammad Mahdi Moeini
Volume 18, Issue 4 , December 2016, Pages 729-740
Abstract
Effects of ethanol and acetone extracts of Glycyrrhiza glabra at six levels (0, 50, 100, 200,300 or 500 µl) were evaluated on gas production process, ruminal fermentation and protozoa population in Markhoz goat. The gas production was conducted by alfalfa substrate using compiled randomized design ...
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Effects of ethanol and acetone extracts of Glycyrrhiza glabra at six levels (0, 50, 100, 200,300 or 500 µl) were evaluated on gas production process, ruminal fermentation and protozoa population in Markhoz goat. The gas production was conducted by alfalfa substrate using compiled randomized design with five replications in each treatment. The fermentation parameters, methane, partitioning factor (PF) and volatile fatty acids (VFA) were recorded. The gas production in 24 h was increased in all levels of both extracts (P˂0.01). The methane production decreased in ethanol extract (P˂ 0.05) however, it was increased by acetone extract at level of 300 and 500 µl (P˂0.01). The organic matter degradability was improved by addition of both extracts (P˂0.01). The Ammonia was declined by ethanol extract (P˂ 0.01). The PF and microbial mass production were decreased by inclusion of both extract, however the VFA and metabolizable energy were enhanced at all levels of both extracts (P˂0.01). All subfamily (except Diplodiniinae)and total protozoa population were decreased by both extracts. It can be concluded that ethanol and acetone extracts of Glycyrrhiza glabra has the potential to improve degradability and were decreased methane production and protozoa population of goat rumen.
Hossein Gholami
Volume 18, Issue 4 , December 2016, Pages 741-748
Abstract
This study was conducted to determine the metabolizable and net energy for lactation of Iranian sainfoin using chemical composition and gas production data obtained by experiments being carried out in different animal nutrition laboratories mainly Animal Science Research Institute (ASRI) (from 1987 to ...
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This study was conducted to determine the metabolizable and net energy for lactation of Iranian sainfoin using chemical composition and gas production data obtained by experiments being carried out in different animal nutrition laboratories mainly Animal Science Research Institute (ASRI) (from 1987 to 2011) in IRAN. Linear fitted equations as well as logarithmic, inverse, quadratic, cubic, power, S and exponential fitted equations for components such as crude protein, crude fat, crude ash, crude fiber, neutral detergent fiber, acid detergent fiber and NFE were estimated. The obtained equations for crude protein were very significant (P<0.001) with high coefficient of the determination (R2 = 0.731). In the above mentioned equations for estimating metabolizable energy of sainfoin hay, crude protein can be chosen as an independent variable. By selecting crude protein as an independent variable and related equations, Metabolizable energy (ME), net energy for lactation (NEL), Net energy for maintenance (NEm) and net energy for growth (NEg) of Iranian sainfoin were 2.43, 1.29, 1.55 and 0.94 Mcal/Kg DM, respectively. The result showed, with a simple chemical experiment of crude protein in the animal nutrition laboratory can be estimated available energy of Iranian sainfoin and used it in ruminant diets.
Ali Kiani; Ayoob Azizi shotor-khoft
Volume 18, Issue 4 , December 2016, Pages 749-760
Abstract
Effects of feeding sequence and boiling of barley on digestibility of fiber and activity of hydrolytic enzymes in colonic contents of horse were investigated. The experiment carried out in a completely randomized design using six Arabian mares in a factorial cross-over with four consecutive periods. ...
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Effects of feeding sequence and boiling of barley on digestibility of fiber and activity of hydrolytic enzymes in colonic contents of horse were investigated. The experiment carried out in a completely randomized design using six Arabian mares in a factorial cross-over with four consecutive periods. The concentrate part of the ration was offered either 30 min after (F-C) or 30 min before (C-F) forage feeding. Barley grain was either boiled (processed) or crushed (not processed). At the end of each period, fecal sample was collected directly from rectum and the activities of carboxymethyl cellulase, microcrystalline cellulase, filter paper degrading, and α-amylase enzyme were determined. Results showed that dry matter, neutral detergent fiber and acid detergent fiber intakes were significantly higher in C- F than those in F-C (P<0.05). Feeding sequence had no effects on digestibility of fiber and on activity of carboxymethyl cellulase, microcrystalline cellulase and filter paper degrading activity. Amylase activity was significantly increased in C-F and decreased by boiling barley (P<0.05). When processed barley was used, the activity of carboxymethyl cellulase decreased in F-C but increased in C-F. In conclusion, using F-C feeding sequence and boiling barley showed positive effects on the activity of hydrolytic enzymes in rectal contents of Aabian mares.
Reza Naseri Harsini; Farokh Kafilzadeh
Volume 18, Issue 4 , December 2016, Pages 761-773
Abstract
Effects of feeding a commercial probiotic (primalac) on performance and carcass characteristics of Morkhoz goat kids were studied using 16 male kids (13.2 ± 1.6 kg body weight, 3 months old) based on a completely randomized design (2 treatments and 8 replicates). For this purpose kids were fed ...
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Effects of feeding a commercial probiotic (primalac) on performance and carcass characteristics of Morkhoz goat kids were studied using 16 male kids (13.2 ± 1.6 kg body weight, 3 months old) based on a completely randomized design (2 treatments and 8 replicates). For this purpose kids were fed for 119 days. The amount of feed consumption was recorded daily and body weight gain and feed conversion ratio were recorded and calculated monthly. Blood samples were collected from jugular vein three times during the experimental period, in equal intervals. At the end of the experimental period animals from each treatment were slaughtered to determine the carcass characteristics. Feeding probiotic did not affect performance parameters including the final live body weight, average feed intake, average daily gain, and feed conversion ratio. With the exception of creatinine concentration, which was lower (P<0.05) in probiotic consuming animals, plasma concentration of other metabolites did not affect by the probiotic consumption. Carcass characteristics measured in this study including weights of live body, carcasss, gastrointestinal tract, liver, heart and internal adipose tissues, weights of neck, rib, loin and long leg cuts and rib eye area did not affect by the treatments and only back fat thickness and shoulder weight were affected (P<0.05). In conclusion, it seems that the probiotic used in this experiment had no effect on performance and carcass chararcteristics of healthy, growing Morkhoz goat kids.
Mohammad reza Asadi; seyed davood sharifi
Volume 18, Issue 4 , December 2016, Pages 775-788
Abstract
Effect of probiotic, prebiotic and synbiotic in protein-deficient diets on performance and intestinal histomorphometry were investigated in a 2 × 4 factorial experiment. The treatments were arranged in two levels of protein (recommended and 10 percent less than requirements), and four types of ...
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Effect of probiotic, prebiotic and synbiotic in protein-deficient diets on performance and intestinal histomorphometry were investigated in a 2 × 4 factorial experiment. The treatments were arranged in two levels of protein (recommended and 10 percent less than requirements), and four types of additives (without additives, probiotics Gallipro, prebiotic Technomos and a mixture of them) in a completely randomized design with eight treatments and four replicates and 25 birds per each. Birds fed on low protein diet had more feed intake and feed conversion ratio (P<0.05). Feed conversion ratio of birds fed on additives (probiotics, Probiotic and synbiotic) diets was lower than of others (P<0.05). The Relative weight of cecea in birds fed on diets containing low protein was higher than of others (P<0.05). An increase in crypt depth of duodenum and a reduction in villi length in jejenum and villi length: crypt depth ratio in deudenum and jejenum were observed by feeding protein dificeint diets (P<0.05). Villi length in the duodenum of birds fed on additive-less diet were lower than of birds fed diets containing prebiotic or mixture of probiotic and prebiotic (P<0.05). Villi length: crypt depth ratio in jejenum of birds fed on diets containing recommended protein and mixture of probiotic and prebiotic were higher than of others (P<0.05). Villi length in ileum were increased by adding the mixture of probiotic and prebiotic to the diet (P<0.05). Villi thickness in the ileum of birds that were received synbiotic was lower than of birds that fed on diets containing prebiotics (P<0.05). It could be concluded that using of probiotic, prebiotic and their mixtre in protein deficient diets, improve the feed conversion ratio in Japanese quail by improving intestinal histomorphometric parameters.
Razieh Badiefar; Farid Shariatmadari
Volume 18, Issue 4 , December 2016, Pages 789-799
Abstract
The aim of this study was to investigate the influence of whole barley with GalyZym poly enzymesupplementation on performance and nutrient digestibility in laying hens. In this study 105 laying hens (w-36) at the age of 40 to 52 weeks were distributed randomly in 5 treatments, with 7 replication, and ...
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The aim of this study was to investigate the influence of whole barley with GalyZym poly enzymesupplementation on performance and nutrient digestibility in laying hens. In this study 105 laying hens (w-36) at the age of 40 to 52 weeks were distributed randomly in 5 treatments, with 7 replication, and 3 birds in each. The experimental treatments included control diet, diets containing barley seed without enzyme, diets containing barley and Galyzym enzyme supplementation,diet containing chopped oatmeal without enzyme, diet containing chopped oatmeal and Galyzym enzyme supplementation. Effects of treatments on production and egg weight was not significant, But laying hens that fed a diet containing barley seeds (with or without enzyme supplementation), had more daily feed intake compared to the control birds (P<0.05). Effects of treatments on egg quality characteristics were not significant. Addition of Galyzym enzyme to the diets containing barley had no effect on apparent metabolizable energy of the diet, dry matter and Organic matter digestibility as well. When enzyme was added to the diets containing oatmeal,an increase of protein digestibility were observed.According toresults of this study, 50 percent of corn in corn and soybean meal based diets on can be replaced with barley grain supplemented with enzyme, without decreasing the performances and digestibility of the nutrients.
Volume 18, Issue 4 , December 2016, Pages 801-810
Abstract
This study was conducted to evaluate the effects of replacing soybean meal with hempseed meal in diet on performance, egg quality and blood parameters in laying hens. A total of 200 Hy-line 102 wks laying hens were randomly allotted to 25 cages in 5 dietary treatments with 5 replicates (8 birds each ...
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This study was conducted to evaluate the effects of replacing soybean meal with hempseed meal in diet on performance, egg quality and blood parameters in laying hens. A total of 200 Hy-line 102 wks laying hens were randomly allotted to 25 cages in 5 dietary treatments with 5 replicates (8 birds each replicate). This experiment was conducted in a completely randomized design with 5 treatments involved different ratio of soybean meal to hempseed meal protein source including 1) ratio of 100: 0 protein of soybean meal to hempseed meal (control); 2) ratio of 75: 25 protein of soybean meal to hempseed meal; 3) ratio of 50: 50 protein of soybean meal to hempseed meal; 4) ratio of 25: 75 protein of soybean meal to hempseed meal; and 5) ratio of 0: 100 protein of soybean meal to hempseed meal. Egg production, egg mean weight and feed intake in the birds fed diet contained ratio of 0: 100 protein of soybean meal to hempseed meal were higher than control diet (P<0.05). Albumin height (Haugh unit), yolk color index, yolk weight percentage and shell weight percentage were ameliorated when hens fed diets the highest protein ratio of hempseed meal compared with the control group. Using hempseed meal in diets decreased (P<0.05) the cholesterol, triglycerides and lipoprotein with low density as compared with the control group. It can be concluded that replacing of 75 % and 100% of hempseed meal to soybean meal may improve the performance, egg quality of post molting layer and decrease the serum LDL and cholesterol of laying hens.
Elham Sarbaz; Bahman navidshad; Farzad Mirzaei Aghjegheshlagh
Volume 18, Issue 4 , December 2016, Pages 811-820
Abstract
The effect of peanut pod as insoluble fiber source in diets on growth performance, small intestine morphology and nutrient digestibility of broilers was evaluated. This study was conducted using 320 Ross-308 broiler chickens in a completely randomized design with four treatments diets (containing 0 (control), ...
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The effect of peanut pod as insoluble fiber source in diets on growth performance, small intestine morphology and nutrient digestibility of broilers was evaluated. This study was conducted using 320 Ross-308 broiler chickens in a completely randomized design with four treatments diets (containing 0 (control), 2.5, five or 7.5 percent peanut pod), with five replicates and 16 birds in each from 11 to 42 days of age. At the growth phase (11 to 24 d) dietary peanut pod decreased feed intake and feed conversion ratio, however, the birds that fed the diet containing 7.5 percent had higher daily weight gain (P<0.05). Peanut pod improved the digestibility coefficients of dry matter and ether extract and the diets containing 2.5 or 5.0 percent peanut pod had more organic matter digestibility than the control diet (P<0.05) Chicks that fed the diets containing 5.0 or 7.5 percent peanut pod, the villous was (P<0.05) longer than control birds. The results of present study suggest that using peanut pod up to 7.5 percent of diet did not adversely affect chickens growth performance and can improve feed conversion ratio at the grower phase.
Volume 18, Issue 4 , December 2016, Pages 821-830
Abstract
An experiment was conducted with 600 one-day-old male broilers (Ross 308) to investigate the effects of Dillaqueous extracts (Anethum graveolens) on blood and growth performance in broiler during a 42-days period. The chickens were divided to four treatments (0, 5, 10 and 20 ml of dill aqueous extracts ...
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An experiment was conducted with 600 one-day-old male broilers (Ross 308) to investigate the effects of Dillaqueous extracts (Anethum graveolens) on blood and growth performance in broiler during a 42-days period. The chickens were divided to four treatments (0, 5, 10 and 20 ml of dill aqueous extracts in drinking water) of five replicates each. Body weight gain, feed intake and feed conversion ratio were measured. At the end of the experiment (day 42), 2 chickens from each replicate were randomly selected and slaughtered. The heart was removed; weights of right and left ventricles were determined separately and ascetic index was calculated. Plasma activity of alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), glutathione peroxidase (GPX), superoxide dismutase (SOD) activity, total antioxidant status (TAS) and Malondialdehyde (MDA) content of plasma were determined. Results showed that 5ml dill aqueous extracts, significantly increased the body weight and reduced the ascetic index, mortality due to ascites and feed conversion ratio (P<0.05). Dill aqueous extracts reduced the plasma glucose level (P<0.05). Dill aqueous extracts at 10 and 20 ml, reduced the plasma triglyceride, lactate dehydrogenase (LDH) activity and increased the plasma HDL (P<0.05). Antioxidant parameters were not significantly affected by treatments. It was concluded that, 5 ml of aqueous extracts of dill could improve the growth performance and reduce the mortality due to ascites in broilers.
Hossein Daghigh Kia; Fatemeh Zare Ghaleh Jigh; Abouzar Najafi; Hossein Vaseghi Dodran
Volume 18, Issue 4 , December 2016, Pages 831-840
Abstract
In order to improve the quality of ram semen after freezing-thawing process, different levels of ethanol extract of Bilberry plants were investigated. In this study five rams were used for semen collection twice a week by an artificial vagina. In order to eliminate the effects of the individual, the ...
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In order to improve the quality of ram semen after freezing-thawing process, different levels of ethanol extract of Bilberry plants were investigated. In this study five rams were used for semen collection twice a week by an artificial vagina. In order to eliminate the effects of the individual, the semen samples were pooled. Different levels of Bilberry extract (0, 100, 150, 200 μl/ml) were added to the Tris- Egg yolk based extender. Following cooling and freezing of semen samples, they were stored in liquid nitrogen until evaluation. After freezing-thawing, motility parameters were evaluated using the Computer-assisted sperm analysis system (CASA), the viability of sperms by Eosin-Nigrosin staining, membrane integrity using the hypo-osmotic swelling test, sperm abnormality by Hancock solution and lipid peroxidation by measuring malondialdehyde concentration. The results showed that the addition of 150 μl of Bilberry extract improved sperm parameters following freezing-thawing process compared to the control group (P< 0.05). According to the results of this study, Bilberry extract can be used as a natural antioxidant, with high performance, inexpensive and available in ram sperm diluent.
Arash Kheradmand
Volume 18, Issue 4 , December 2016, Pages 841-852
Abstract
This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin ...
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This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin C, and control group without any antioxidant. Total sperm motility, progressive motility and HOS test were assessed at h 0, 24 and 48. Using 4 mg of BSA and 5 IU of vitamin E increased sperm motility and plasma membrane integrity compared to other antioxidants (P<0.05). Although, higher concentrations of these antioxidants relatively enhanced sperm quality parameters, however, it was still greater than the control group (P<0.05). Different concentrations of vitamin C did not affect sperm quality parameters. According to the results of the present study, addition of BSA and vitamin E to buck semen improved total and progressive sperm motility as well as plasma membrane integrity during 48 h after incubation at 5 °C.
behrooz shokrani; morteza mehri; amir fattah; mohsen sharafi; fatemeh shirmohammad
Volume 18, Issue 4 , December 2016, Pages 853-865
Abstract
Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates ...
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Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates per each group. Semen samples were collected on days 0, 14, 28, 42 and 56 of experiment. Results showed that 100mg rosemary significantly improved several traits of sperm such as Amplitude of Lateral Head Displacement (ALH), Average Path Velocity (VAP), and Straight Line Velocity (VSL) on days 42 and 56, and sperm Membrane Integrity (MI), sperm viability, Linearity (LIN) and Total Motility (TM) on day 56 (P<0.05) and significantly decreased dead sperms (P<0.05). However, sperm concentration, apoptosis and morphological changes, Sperm Track Straightness (STR), Curvilinear Velocity (VCL), Progressive motility (PM) and malondialdehyde (MDA) level were not affected by experimental treatment. It can be concluded that 100 mg rosemary essential oil/kg of diet could be efficient for improvement of some qualitative sperm parameters.
Volume 18, Issue 4 , December 2016, Pages 867-876
Abstract
This research was conducted to investigate the effect of different concentrations of royal jelly (RJ) as an antioxidant source on in vitro maturation of goat oocytes. Goat ovaries were collected from a local abattoir. Cumulus-oocyte-complexes (COCs) were recovered from antral follicles. The COCs were ...
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This research was conducted to investigate the effect of different concentrations of royal jelly (RJ) as an antioxidant source on in vitro maturation of goat oocytes. Goat ovaries were collected from a local abattoir. Cumulus-oocyte-complexes (COCs) were recovered from antral follicles. The COCs were placed in maturation medium and were reached to MII stage after 24 hours. The results indicated that the increasing of royal jelly concentration in the maturation media was significantly increased maturation rate of oocytes from control (54 ± 0%) to 20 mg/mL group (93 ± 0.5%). Also, the higher RJ concentrations were associated with increased cleavage (62.5 ± 2.64% to 85.6 ± 3.78%) and blastocyst rate (15.4 ± 2.26% to 38.6 ± 3.42%) from the RJ0 to the RJ20 group. Addition of RJ was significantly (P<0.05) increased the antioxidant gene expressions in the oocytes. In conclusion, supplementation of 20 mg/mL royal jelly to the maturation media was significantly improved the redox state, nuclear maturation and blastocyst formation.
Abbas Masoudi; Arash Azarfar
Volume 18, Issue 4 , December 2016, Pages 877-888
Abstract
In the current study, growth parameters of broiler chickens fed with rice hull were estimated and their final body weigh was predicted using non-linear, spline regression and neural networks models. The experimental treatments were control and dietary inclusion of rice hull at the levels of 2.5, 5 and ...
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In the current study, growth parameters of broiler chickens fed with rice hull were estimated and their final body weigh was predicted using non-linear, spline regression and neural networks models. The experimental treatments were control and dietary inclusion of rice hull at the levels of 2.5, 5 and 7.5 percent. Predicted final body weight estimated by non-linear regression models in the current study was higher in control chicks compare with those fed rice hull containing diets (P<0.05), but similar among the other birds. Inflection point of growth curve occurred earlier in in control chicks than those fed hull rice containing diets (P<0.05), but increasing hull rice in the diet level had no effect on this parameter. The highest and lowest body weight at inflection point observed in birds fed control and those fed diet containing 5 percent of rice hull, respectively (P<0.05). Feeding chicks with diets containing rice hull decreased parameters b of spline regression model compared with control birds, while parameter c was only lower in birds fed diet containing 7.5 percent hull rice compared with birds on control diet (P<0.05). According to our results, spline regression model is more efficient than the non-linear and artificial neural network models to predict body weight of broiler chicks fed with diets containing rice hull at day 42 of age.