Document Type : Research Paper
Authors
Abstract
In order to improve the quality of ram semen after freezing-thawing process, different levels of ethanol extract of Bilberry plants were investigated. In this study five rams were used for semen collection twice a week by an artificial vagina. In order to eliminate the effects of the individual, the semen samples were pooled. Different levels of Bilberry extract (0, 100, 150, 200 μl/ml) were added to the Tris- Egg yolk based extender. Following cooling and freezing of semen samples, they were stored in liquid nitrogen until evaluation. After freezing-thawing, motility parameters were evaluated using the Computer-assisted sperm analysis system (CASA), the viability of sperms by Eosin-Nigrosin staining, membrane integrity using the hypo-osmotic swelling test, sperm abnormality by Hancock solution and lipid peroxidation by measuring malondialdehyde concentration. The results showed that the addition of 150 μl of Bilberry extract improved sperm parameters following freezing-thawing process compared to the control group (P< 0.05). According to the results of this study, Bilberry extract can be used as a natural antioxidant, with high performance, inexpensive and available in ram sperm diluent.
Keywords
- شهباززاده ر، دقیقکیا ح، مقدم غ، دهقان غ، حسین‌خانی ع و اشرفی ا (1394) تأثیر سطوح مختلف عصاره الکلی مرزه سهندی بر کیفیت اسپرم منجمد/ یخگشایی شده گاو هلشتاین. پژوهشهای علوم دامی. 25(1): 24-13.
- فرهادی ر، دقیقکیا ح و اشرفی ا (1394) تأثیر عصاره اتانولی مریم‌گلی سهندی به عنوان آنتیاکسیدان طبیعی بر پارامترهای کیفی اسپرم منجمد/ یخگشایی شده گاو هلشتاین. پژوهشهای تولیدات دامی. 12: 79-86.
3. Aitken RJ (1995) Free radicals, lipid peroxidation and sperm function. Reproduction, Fertility, and Development 7(4): 659-668.
4. Baghshahi H‚ Riasi A‚ Mahdavi AH and Shirazi A (2014) Antioxidant effect of clove bud (syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation. Cryobiology. 69(3): 482-487.
5. Dragana M‚ Stanisavljević, Saša S‚ Stojiĉević, Sofija M‚ ĐorĊević, Branislav P‚ Zlatković, Dragan T‚ Veliĉković, Ivana T‚ Karabegović, Miodrag L and Lazić (2012) Antioxidant activity, the content of total Phenols and flavonoids in the ethanol extracts of mentha longifolia L. Hudson dried by the use of Different techniques. Chemical Industry and Chemical Engineering Quarterly. 18(3): 411-420.
6. Emamverdi M, Zhandi M, Zare-Shahneh A, Sharafi M and Akbari-Sharif A (2013) Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based extender. Reproduction in Domestic Animals. 48(6): 899-904.
7. Ersoy N, Bagci Y and Gok V (2011) Antioxidant properties of 12 cornelian cherry (Cornus mas L.) fruit types selected from Turkey. Scientific Research and Essays. 6(1): 98-102.
8. Esteraauer H and Cheeseman KH (1990) Determination of Aldehydic Lipid Peroxidation Products: Malonaldehyde and 4-Hydroxynonenal. Methods in Enzymology. 186: 407-421.
9. Evans G, Maxwell WMC and Salamon S (1987) Artificial Insemination of Sheep and Goats. Butterworths, Sydney.
10. Forouzanfar M, Fazilati M and Hosseini S (2007) Investigation of different glycerol and Egg Yolk concentration on freezing Bakhtiari Ram Semen. Journal of Iranian Anatomical Sciences. 5(18): 17-25.
11. Gil L, Mascaró F, Mur P, Gale I, Silva A, González N, Malo C and Cano R (2010) Freezing ram semen: The effect of combination of soya and rosemary essences as a freezing extender on post-thaw sperm motility. Reproduction in Domestic Animals. 45: 91.
12. González N, Gil L, Martinez F, Malo C, Cano R, Mur P and Espinosa E (2010) Effect of natural antioxidant Rosemary in canine soya freezing extender. Reproduction in Domestic Animals. 45: 88.
13. Hassanpour H, Hamidoghli Y and Adlipour M (2011) Antioxidant capacity and phytochemical properties of cornelian cherry (Cornus mas L.) genotypes in Iran. Scientia Horticulturae. 129(3): 459-463.
14. Hu JH, Li QW, Zhang T and Jiang ZL (2009) Effect of Gynostemma pen-taphyllum polysaccharide on boar spermatozoa quality following freezing–thawing. Cryobiology. 59(3): 244 249.
15. Malo C, Bathgate R, Gil L and De Graaf SP (2010) Influence of natural antioxidants on boar spermatozoa quality and DNA integrity of frozen-thawed boar semen. Reproduction in Domestic Animals. 45: 98.
16. Malo C, Gil L, Gonzalez N, Martínez F, Cano R, de Blas I and Espinosa E (2010b) Anti-oxidant supplementation improves boar sperm characteristics and fertility after cryopreservation: Comparison between cysteine and rosemary (Rosmarinus officinalis). Cryobiology. 61(1): 142-147.
17. Malo C, Gil L, Cano R, Martínez F and Galé I (2011) Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology. 75(9): 1735-1741.
18. Martinez-Pastor F, Fernandez-Santos MR, Domınguez Rebol-ledo A, Esteso MC and Garde JJ (2009) DNA status on thawed semen from fighting bull: a comparison between the SCD and the SCSA tests. Reproduction in Domestic Animals. 44(3): 424-431.
19. Maxwell WMC and Watson PF (1996) Recent progress in the preservation of ram semen. Animal Reproduction Science. 42(1-4): 55-65.
20. Otakar R‚ Jira M‚ Daniela K and Tunde J (2010) Selected cultivars of cornelian cherry (cornus mas) as a new food source for human nutrition. African Journal of Biothecnology. 9(8): 1205-1210.
21. Sadeghi Ghotbabadi F, Alizadeh A, Zadehbagheri M, Kamelmanesh MM and Shaabani M (2012) Phytochemical composition of the essential oil, total phenolic content, antioxidant and antimicrobial activity in Iranian Satureja Sahendica Bornm at different ontogenesis conditions. Journal of Medicinal Plants Research. 6(19): 3525-3534.
22. Vaseghi Dodaran H, Zhandi M, Sharafi M, Nejati-Amiri E, Nejati-Javaremi A, Mohammadi Sangcheshmeh A, Shehab-El-Deen MA and Malak Shakeri (2015) Effect of ethanol induced mild stress on post thawed bull sperm quality. Cryobiology. 71(1): 12-17.
23. Zhao HW, Li QW, Ning GZ, Han ZS, Jiang ZL and Duan YF (2009) Rhodiola sacra aqueous extract (RSAE) improves biochemical and sperm characteristics in cryopreserved boar semen. Theriogenology. 71(5): 849-57.
24. Zanganeh Z, Zhandi M, Zare Shahneh A, Najafi A, Mahdi Nabi M and Mohammadi Sangcheshmeh A (2013) Does rosemary aqueous extract improve buck semen cryopreservation? Small Ruminant Research. 114(1): 120-125.
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