Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X

Effect of royal jelly on gene expression of antioxidant enzymes in in vitro maturation and embryo development of goat oocytes

Document Type : Research Paper

https://doi.org/10.22059/jap.2016.58360
Abstract
This research was conducted to investigate the effect of different concentrations of royal jelly (RJ) as an antioxidant source on in vitro maturation of goat oocytes. Goat ovaries were collected from a local abattoir. Cumulus-oocyte-complexes (COCs) were recovered from antral follicles. The COCs were placed in maturation medium and were reached to MII stage after 24 hours. The results indicated that the increasing of royal jelly concentration in the maturation media was significantly increased maturation rate of oocytes from control (54 ± 0%) to 20 mg/mL group (93 ± 0.5%). Also, the higher RJ concentrations were associated with increased cleavage (62.5 ± 2.64% to 85.6 ± 3.78%) and blastocyst rate (15.4 ± 2.26% to 38.6 ± 3.42%) from the RJ0 to the RJ20 group. Addition of RJ was significantly (P<0.05) increased the antioxidant gene expressions in the oocytes. In conclusion, supplementation of 20 mg/mL royal jelly to the maturation media was significantly improved the redox state, nuclear maturation and blastocyst formation.

Keywords

Antioxidant Enzymes
Embryonic development
Goat oocytes
in vitro maturation
Royal jelly
  1. Abd-Allah and Saber Mohamed (2012) Effect of Royal Jelly on viability and in vitro Maturation of Egyptian sheep oocytes in serum supplemented Medium. British Journal of Pharmacology Toxicology. 3: 29-32.
  2. Ali AA, Bilodeau JF and Sirard MA (2003) Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development.Theriogenology. 59: 939-949.
  3. Aslan A, Cemek M, Buyukokuroglu ME, Altunbas K, Bas O and Yurumez Y (2012) Royal jelly can diminish secondary neuronal damage after experimental spinal cord injury in rabbits. Food and Chemical Toxicology. 50: 2554-2559.
  4. Bolojan L (2012) Characterization of free radicals in biomedical andbiopharmaceutical systems. Ph.D. Thesis, University of Cluj-Napoca. Pp. 1-30.
  5. Bormann CL, Ongeri EM and Krisher RL (2003) The effect of vitamins during maturation of caprine oocytes on subsequent developmental potential in vitro. Theriogenology. 59: 1373-1380.
  6. Boukraa L, Niar A, Benbarek H and Benhanifia M (2008) Additive action of royal jelly and honey against Staphylococcus aureus. Medicinal Food. 11: 190-192.
  7. Cetica PD, Pintos LN, Dalvit GC and Beconi MT (2001) Antioxidant Enzyme Activity and Oxidative Stress in Bovine Oocyte in vitro Maturation. International Union of Biochemistry and Molecular Biology Life. 51: 57-64.
  8. Guo H, Kouzuma Y and Yonekura M (2008a) Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chemistry. 113: 238-245.
  9. Guo H, Ekusa A, Iwai K, Yonekura M, Takahata Y and Morimatsu F (2008b) Royal Jelly peptides inhibit lipid peroxidation in vitro and in vivo. Journal of Nutritional ScienceVitaminol. 54: 191-195.
  10. Ikeda S, Kitagawa M, Imai H and Yamada M (2005) The roles of vitamin A for cytoplasmic maturation of bovine oocytes. Journal of Reproduction and Development. 51: 23-3.
  11. Jamnik P, Goranovic D and Raspor P (2007) Antioxidative action of royal jelly in the yeast cell. Experimental Gerontology. 42: 594-600.
  12. Kamakura M, Maebuchi M, Ozasa S, Komori M, Ogawa T, Sakaki T and Moriyama T (2005) Influence of royal jelly on mouse hepatic gene expression and safety assessment with a DNA microarray. NutritionalScienceand Vitaminology. 51: 148-155.
  13. Kanbur M, Eraslan G, Beyaz L, Silici S, Liman BC, Altınordulu S and Atasever A (2009) The effects of royal jelly on liver damage induced by paracetamol in mice. Experimental and ToxicologicPathology. 61: 123-132.
  14. Karadeniz A, Simsek N, Karakus E, Yildirim S, Kara A, Can I, Kisa F, Emre H and Turkeli M (2011) Royal Jelly Modulates Oxidative Stress and Apoptosis in Liver and Kidneys of Rats Treated with Cisplatin. Oxidative Medicine and Cellular Longevity 2011, 10 pages.
  15. Kimura M, Kimura Y, Tsumura K, Okihara K, Sugimoto H, Yamada H and Yonekura M (2003) 350-kDa royal jelly glycoprotein (apisin), which stimulates proliferation of human monocytes, bears the beta 1-3galactosylated Nglycan: Analysis of the N-glycosylation site. Bioscience, Biotechnology and Biochemistry. 67: 2055-2058.
  16. Kodai T, Umebayashi K, Nakatani T, Ishiyama K and Noda N (2007) Compositions of royal jelly II.Organic acid glycosides and sterols of the royal jellyof honeybees. Apis mellifera. 55: 1528-1531.
  17. Kohno K, Okamoto I, Sano O, Arai N, Iwaki K, Ikeda M and Kurimoto M (2004) Royal jelly inhibits the production of proinflammatory cytokines by activated macrophages. Bioscience Biotechnology and Biochemistry. 68: 138-145.
  18. Krishnamurthy P and Wadhwani A (2012) Antioxidant Enzymes and Human Health, In: El-Missiry, MA. (ed.), 2012. Antioxidant Enzyme, Published by InTech. Chapter 1. Pp. 1-16.
  19. Kucuk M, Kolayli S, Karaoglu S, Ulusoy E, Baltaci C and Candan F (2007) Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry. 100: 526-534.
  20. Lamina S, Ezema CHI, Theresa AI and Anthonia EU (2013) Effects of free radicals and antioxidants on exercise performance. Journal of Medical Science. 2: 83-91.
  21. Mates JM (2000) Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology. 153: 83-104.
  22. Martos M, Navajas YR, Lopez JF and Alvarez JAP (2008) Functional properties of honey, propolis, and royal jelly, Journal of Food Science. 73: 117-124.
  23. Mazangi HR, Deldar H, Kashan NE and Mohammadi-Sangcheshmeh A (2015) Royal jelly treatment during oocyte maturation improves in vitro meiotic competence of goat oocytes by influencing intracellular glutathione synthesis and apoptosis gene expression Reproduction, Fertility and Development. 27: 241-241.
  24. Motlagh MK, Shahneh AZ, Daliri M, Kohram H and Ghragozlou F (2008) In vitro maturation of sheep oocytes in different concentrations of mare serum. African Journal of Biotechnology. 7: 3380-3382.
  25. Pavel CI, Marghitas LAO, Bobis DS, Dezmirian A, Sapcaliu I, Radoi and Madas MN (2011) Biological activities of royal jelly. Animal Science and Biotechnology. 44: 108-118.
  26. Pourmoradian S, Mahdavi R, Mobasseri M, Faramarzi E and Mobasseri M (2012) Effects of royal Jelly supplementation on body weight and dietary intake in type 2 diabetic females. Health Promotion Perspectives. 2: 231-235.
  27. Rahman ANMA, Abdullah RB and Wan-Khadijah WE (2008) In vitro maturation of oocytes with special reference to goat: A review. Biotechnology. 7: 599-611.
  28. Rezaei N and Chian RC (2005) Effects of essential and non-essential amino acids on in vitro maturation, fertilization and development of immature bovine oocyte. Reproductive Medicine. 3: 36-41.
  29.  Sabatin AG, Marcazzan G, Caboni MF, Bogdanov S and Muradian LB (2009) Quality and standardization of royal jelly. Journalof Agri-Food and Applied Sciences. 1: 1-6.
  30.  Tatemoto H, Muto N, Sunagawa I and Nakada T (2004) Protection of porcine oocytes against cell damage caused by oxidative stress during in vitro maturation: role of superoxide dismutase activity in porcine follicular fluid. Biology of Reproduction. 71: 1150-1157.
  31. Vahedi AV, Zeinoaldini S, Kohram H and Farahavar A (2009) Effects retinoic acid on nuclear maturation of bovine oocytes in vitro. African Journal of Biotechnology. 8: 3974-3978.
  32. Whitaker BD (2007) Oxidative stress mechanisms within the developing  porcine oocyte and the effects of Antioxidant supplementation. Ph.D. Thesis, Faculty of the Virginia Polytechnic. Pp. 1-82.
  33. Livak KJ and Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-Δ ΔCT method. Methods. 25: 402-408.
  34. Kridli RT, Husein MQ and Humphrey WD (2003) Effect of royal jelly and GnRH on the estrussyn chronization and pregnancy rate in ewes using intravaginal sponges. Small Ruminant Research.49: 25-30.
  35. Mishima S, Suzuki KM, Isohama Y, Kuratsu N, Araki Y, Inoue M and et al. (2005) Royal jelly has estrogeniceffects in vitro and in vivo. Journal of Ethnopharmacology. 101: 215-220.

Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X
Volume 18, Issue 4
Autumn 2016
Pages 867-876

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us