نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی ایران
2 دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، تخصص: تغذیه نشخوارکنندگان/ میکروبیولوِزی شکمبه/ آشنایی کامل با روشهای آزمایشگاهی
3 3استاد گروه زیست دانشکده علوم، و مرکز تحقیقاتی علوم زیستی و بیوتکنولوژی دانشگاه شهید چمران اهواز 3 Biotechnologies and Biological Science Research Center,
4 Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani- Iran.
5 دانشیار گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه کشاورزی و منابع طبیعی ساری
چکیده
این آزمایش با هدف عمل‌آوری کاه گندم توسط باکتریهای سلولولیتیک جداشده از دستگاه گوارش اسب و تأثیر این باکتریها بر عملکرد برههای پرواری، قابلیت هضم، فراسنجه‌های تخمیری و خونی انجام شد. از 30 رأس بره نر افشاری با میانگین وزن 3±32 کیلوگرم و سن چهار ماه در قالب طرح کاملاً تصادفی استفاده شد. تیمارهای آزمایشی شامل جیرههای حاوی کاه گندم فرآوری شده به‌مدت شش هفته با چهار سویه باکتریایی جداشده از دستگاه گوارش اسب (Paenibacilluspolymyxa L11، Paenibacilluspolymyxa L12، Enterobacter cloacae L2 و Escherichia coli Z2) و جیره شاهد بودند. نتایج نشان داد که فرآوری باکتریایی باعث افزایش محتوای پروتئین و کاهش NDF، iNDF و ADF و هم‌چنین افزایش قابلیت هضم ماده آلی و انرژی متابولیسمی کاه گندم نسبت به شاهد شد. بیش‌ترین مقدار قابلیت هضم ماده آلی و انرژی متابولیسمی به تیمار L11 اختصاص داشت. قابلیت هضم ظاهری ماده خشک، NDF،ADF، غلظت و نسبت اسیدهای چرب فرّار شکمبه، pH و نیتروژن آمونیاکی شکمبه تحت تأثیر فرآوری باکتریایی قرار گرفتند. بیش‌ترین قابلیت هضم مواد مغذی به تیمار L11 و کم‌ترین مقدار به تیمار شاهد اختصاص داشت. کم‌ترین مقدار نیتروژن آمونیاکی شکمبه مربوط به تیمارL11 بود. تیمارهای باکتریایی باعث افزایش غلظت کل اسیدهای چرب، غلظت پروپیونات و کاهش غلظت استات شکمبه شدند. با توجه به نتایج، فرآوری کاه گندم با باکتریهای سلولولیتیک جداشده از دستگاه گوارش اسب (به‌ویژه L11) باعث بهبود ارزش غذایی کاه گندم شد. بنابراین به‌نظر می‌رسد فرآوری باکتریایی می‌تواند راه‌کار مناسبی برای استفاده بهتر از بقایای زراعی با ارزش غذایی پایین باشد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of wheat straw processed with cellulolytic bacteria isolated from gastrointestinal tract of horse on performance, digestibility, and fermentation and blood parameters in the fattening lambs
نویسندگان [English]
- Maryam Harsini 1
- tahereh mohammadabadi 2
- hosein Motamedi 3
- Mohsen Sari 4
- Asadolah Teimouri Yansari 5
1 12 Department of Animal Science, Agricultural Sciences and Natural Resources University of Khuzestan, P.O. Box 63517-73637, Mollasani, Ahvaz, Iran
2 Ramin Agriculture and Natural Resources University of Khuzestan, Ahwaz, Mollasani - Iran
3 3 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, P.O. Box 61357-43169, Khuzestan, Iran. 3 Biotechnologies and Biological Science Research Center, Shahid Chamran University of Ahvaz, P.O. Box 61357-43169, Khuzestan, Ir
4 Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani- Iran.
5 4 Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 48181-68984, Sari, Iran
چکیده [English]
effect of these bacteriaon the performance of fattening lambs, digestibility, fermentation and blood parameters. Thirty Afshari male lambs with an average weight of 32 ± 3 kg and age of four months were used in a completely randomized design. The experimental treatments included diets containing wheat straw processed with four bacterial strains isolated from the gastrointestinal tract of horse (Paenibacillus polymyxa L11, Paenibacillus polymyxa L12Enterobacter cloacae L2 and Escherichia coli Z2) for 6 weeks; and the control treatment. The results showed that bacterial processing increased crude protein and decreased NDF, iNDF and ADF content, as well as increased organic matter digestibility (OMD) and metabolizable energy (ME) of wheat straw compared to the control. The highest amount of OMD and ME was assigned toL11. Apparent digestibility of DM, NDF and ADF, concentrations and ratios of ruminal volatile fatty acid (VFA), ruminal pH and ammonia nitrogen were affected by bacterial processing. The highest nutrient digestibility was assigned to the L11 and and the lowest amount was assigned to the control treatment. The lowest amount of ruminal ammonia nitrogen was observed in L11 treatment. Bacterial treatments increased total VFA, propionate concentration and decreased rumen acetate concentration. According to the results, processing of wheat straw with cellulolytic bacteria isolated from the horse's gastrointestinal tract (especially L11) improved nutritional value of wheat straw. Therefore, it seems that bacterial processing can be a suitable strategyfor better use of crop by-product with low nutritional value.
کلیدواژه‌ها [English]
- Bacteria
- digestibility
- performance
- processing
- wheat straw
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