نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد،گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، خوزستان، ایران
2 استاد،گروه علوم دامی، دانشکدۀ علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان
3 دانشیار،گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان-دانشگاه شیروان
چکیده
بهمنظور بررسی تأثیر ویتامین E و نانولیپوزوم ویتامین E بر بیان ژن STAR در بیضه و ژن TSPO در تخمدان بلدرچین مولد ژاپنی، آزمایشی با استفاده از 864 قطعه بلدرچین به‌مدت 10 هفته در قالب طرح کاملاً تصادفی با شش تیمار، شش تکرار و 24 قطعه بلدرچین مولد (16 ماده و هشت نر) در هر تکرار به انجام رسید. تیمارها از جیره حاوی سطوح مختلف ویتامین E (25، 50 و 100 IU در کیلوگرم جیره) و نانولیپوزم ویتامین E (25، 50 و 100 IU در کیلوگرم جیره) تغذیه کردند. نتایج این پژوهش نشان داد، اثر تیمارهای آزمایشی بر بیان ژن TSPO در تخمدان و ژن STAR در بیضه معنیدار بوده است (05/0>P). افزودن سطح 50 IU ویتامین E بیان ژن TSPO در تخمدان را نسبت به تیمار شاهد بهطور معنیداری افزایش داد (05/0>P). هم‌چنین نتایج نشان داد سطح 25 IU نانولیپوزوم ویتامین E باعث افزایش بیان ژن TSPO در تخمدان شد. این افزایش تفاوت معنیداری با تیمار شاهد نداشت. استفاده از سطوح ویتامین E و سطوح نانولیپوزوم ویتامین E باعث کاهش معنیدار بیان ژن STAR در بیضه بلدرچین ژاپنی شد (05/0>P). براساس نتایج این مطالعه اضافهنمودن سطح 25 IU نانولیپوزوم ویتامین E و هم‌چنین سطوح 50 و 100 IU ویتامین E تأثیر زیادی بر بیان ژن TSPO در تخمدان که از ژن‌های مؤثر بر باروری و تولید مثل است دارد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of vitamin E and vitamin E nanoliposomes on STAR gene expression in testis and TSPO gene in ovary of Japanese quail
نویسندگان [English]
- Fatemeh Hendijani 1
- Jamal Fayazi 2
- Hedayat-allah Roshanfekr 2
- Mohammad Reza Ghorbani 3
1 Animal Science Department, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 Animal Science Department, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
3 Animal Science Department, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده [English]
In order to investigate the effect of vitamin E and vitamin E nanoliposomes on the expression of the STAR gene in testis and TSPO gene in the ovary of Japanese quail, an experiment using 864 pieces of quail for 10 weeks in a completely randomized design with six treatments, six replications and 24 Breeding quail (16 females and eight males) was performed in each replication. The treatments were fed diets containing different levels of vitamin E (25, 50 and 100 IU per kg of diet) and vitamin E nanoliposomes (25, 50 and 100 IU per kg of diet). The results of this study showed that the effect of the experimental treatments on the expression of TSPO gene in the ovary and STAR gene in the testis was significant (P<0.05). The addition of 50 IU of vitamin E significantly increased TSPO gene expression in the ovary compared to the control treatment (P<0.05). The results also showed that the level of 25 IU nanoliposome of vitamin E increased the expression of TSPO gene in the ovary, which was not significantly different from the control treatment. The use of vitamin E and vitamin E nanoliposome levels significantly decreased the expression of STAR gene in the testis of Japanese quail (P<0.05). According to the results of this study, the addition of 25 IU vitamin E nanoliposomes as well as 50 and 100 IU levels of vitamin E has a significant effect on the expression of TSPO gene in the ovary, which is one of the genes affecting fertility and reproduction.
کلیدواژه‌ها [English]
- Nanoliposomes
- Quail
- STAR gene
- TSPO gene
- Vitamin E
Enriched Yeast Modifies the Effects of
Methandienone in Male Rabbits on the HPA
Axis and Adrenal Gland Oxidative Stress. AlAnbar Journal of Veterinary Sciences 13(2).
2. Al-Tawash A, Sami A, Shahneh Z, Ansari M
and Deldar (2019) Improvement of histological
parameters and relative expression of StAR
gene in testis of chrysin-fed roosters. Iranian
Animal Sciences 50 (3): 207-215.
3. Amorezae S, Farzinpour A, Farshad A and
Razmkabir M (2015) The effects of dietary zinc
oxide nanoparticles on some reproductive
parameters of male quail. Research Journal of
Poultry Sciences 1(2): 21-31.
4. Aprioku JS (2013) Pharmacology of free
radicals and the impact of reactive oxygen
species on the testis. Journal Reprod Infertil
14(4): 158-72.
5. Arabpour Z, Mohammadabadi M and Khezri A
(2021) The expression pattern of p32 gene in
femur, humeral muscle, back muscle and back
fat tissues of Kermani lambs. Agricultural
Biotechnology Journal 13 (4):183-200.
6. BAR-Ami S, Amiri Z, Fares F and Gavish M
(1994) Modulation of peripheral benzodiazepine
receptors infemale rat genital organs by various
gonadal steroids. Life Sciences 54(25): 1965-
75.
7. Biswas A, Mohan J, Sastry KVH and Tyagi JS
(2007) Effect of dietary vitamin E on the cloacal
gland, foam and semen characteristics of male
Japanese quail. Theriogenology 67(2): 259-263.
8. Bogan RL, Davis TL and Niswender GD (2007)
Peripheral-type benzodiazepine receptor (PBR)
aggregation and absence of steroidogenic acute
regulatory protein (STAR)/PBR association in
the mitochondrial membrane as determined by
bioluminescence resonance energy transfer
(BRET). The Journal of steroid biochemistry
and molecular biology 104(1-2): 61-67.
9. David B Warheit (2008) How meaningful are
the results of nanotoxicity studies in the absence
of adequate material characterization?.
Toxicological sciences 101(2): 183-185.
10. Khan I, Yousaf S, Subramanian S, Korale O,
Alhnan MA, Ahmed W and Elhissi A (2015)
Proliposome powders prepared using a slurry
method for the generation of beclometasone
dipropionate liposomes. International journal of
pharmaceutics 496(2): 342-350.
11. Li F, Liu J, Liu N, Kuhn LA, Garavito RM and
Ferguson-Miller S (2016) Translocator protein
18 kDa (TSPO): an old protein with new
functions. Biochemistry 55(20): 2821-2831.
12. Lin YF, Chang SJ and Hsu AL (2004) Effects of
supplemental vitamin E during the laying period
on the reproductive performance of Taiwan native
chickens. British Poultry Science 45 (6): 807-814.
13. McCune RW, Roberts S and Young PL (1970)
Competitive inhibition of adrenal Δ5-3βhydroxysteroid dehydrogenase and Δ5-3-
ketosteroid isomerase activities by adenosine 3',
5'-monophosphate. Journal of Biological
Chemistry 245(15): 3859-3867.
14. Modaresi M, Messripour M and Rajaei R (2010)
Effect of cinnamon extract on the number of
spermatocyte and spermatozoa cells in mice.
Iranian Journal of Medicinal and Aromatic
Plants 26 (1): 83-90. (In Persian).
15. Moghimipour M, Kouchak M and Bahmandar R
(2013) Nano-liposomes as new Drug Delivery
Carriers. Jundishapur Science Med Journal, 12
(5): 467-483.
16. Mohammadabadi MR (2020) Expression of
ESR1 gene in Raini Cashmere goat using Real
Time PCR. Agric Biotechnol Journal 12 (1):
177-192. (In Persian).
17. Mohammadabadi MR. and Tohidinejad F
(2017) Charachteristics determination of Rheb
gene and protein in Raini Cashmere goat. Iran J
Appl Anim Sci 7: 289-295.
18. Mozafari MR and Mortazavi SM (2005)
Nanoliposomes: From Fundamentals to Recent
Developments, Trafford Publishing, Ltd,
Oxford, UK. ISBN 1(4120): 5545-8. 19. Nikolaidis MG, Kerksick CM, Lamprecht M
and McAnulty SR (2012) Does vitamin C and E
supplementation impair the favorable
adaptations of regular exercise? Oxidative medicine and cellular longevity.20. Powers SK and Jackson MJ (2008) Exerciseinduced oxidative stress: cellular mechanisms
and impact on muscle force production.
Physiological reviews 88(4): 1243-1276.
21. Sahin T, Kaga I, Unal Y and Elmail DA (2008)
Dietary supplementation of probiotic and
prebiotic combination (Combiotics) on
performance, carcass quality and blood
parameters in growing quails. Journal of Animal
and Veterinary Advances 7(11): 1370-1373.
22. Shi L, Song R, Yao X, Duan Y, Ren Y, Zhang
C and Lei F (2018) Effects of maternal dietary
selenium (Se-enriched yeast) on testis
development, testosterone level and testicular
steroidogenesis-related gene expression of their
male kids in Taihang Black Goats.
Theriogenology 114: 95-102.
23. Široká Z and Drastichova J (2004) Biochemical
marker of aquatic environment contaminationcytochrome P450 in fish. A review. Acta
Veterinaria Brno, 73(1): 123-132.
24. Surai PF, Kutz E, Wishart GJ, Noble RC and
Speake B.K (1997) The relationship between
the dietary provision of α-tocopherol and the
concentration of this vitamin in the semen of
chicken: Effects on lipid composition and
susceptibility to peroxidation. Journal of
reproduction and fertility 110: 47-51.
25. Tabatabai Vakili S, Darabi A, Aghaei A and
Mehrnia M.A (2021) The effect of injecting
different levels of thyme essential oil
nanoemulsion into Japanese quail eggs on
reproductive performance and blood parameters.
Animal Science Research (Agricultural Science)
31 (2): 87-99.
26. Turner TT and Lysiak JJ (2008) Oxidative stress: a
common factor in testicular dysfunction. Journal
of andrology 29 (5): 488-498.
27. Zirkin BR and Chen H (2000) Regulation of
Leydig cell steroidogenic function during aging.
Biology of reproduction 63(4): 977-98.
)