نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانش‌آموخته کارشناسی ارشد،گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران.
2 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران.
3 استادیار، گروه علوم پایه، دانشکده دامپزشکی،دانشگاه شیراز، شیراز، ایران
چکیده
تأثیرپودر برگ رزماری روی فراسنجههای منی و نرخ آسیب DNA تحت شرایط تنش گرمایی با استفاده از 40 قطعه خروس بومی در سن 42 هفته و وزن2300 گرمدرقالب آزمایش فاکتوریل 2×2 طرح بلوک کامل تصادفی با دو سطح درجه حرارت(طبیعی و تنش گرمایی) و دوسطح پودررزماری (بدون رزماریو 7/5 گرم پودررزماری درکیلوگرم جیره)به‌مدت هفت هفته بررسی شد.دمای سالن در شرایط طبیعی22-18و در تنش گرمایی 2±28 درجه سلسیوس تنظیم شد. بعد از دو هفته عادت‌پذیری، به‌مدت پنج هفته، هر هفته دوباراز طریق ماساژ شکمی- پشتی، از پرندگاننمونه منی گرفته شد. نمونههای منی، از نظر فراسنجههای تحرک، زندهمانی و نرخ قطعهقطعهشدن DNA اسپرم ارزیابی شدند. نتایج نشان داد درجه حرارت تنشزا کاهش معنیداری روی حرکت پیشرونده (نوع A)ایجاد کرد و با افزودن پودر رزماری، زندهمانی اسپرم افزایش یافت (p>0/05). افزودن پودر رزماری دردمای طبیعی نرخ قطعهقطعهشدن DNA را به‌طور معنیداری کاهش داد. درخروسهای تحت تنش گرمایی، نرخ قطعهقطعهشدن DNA اسپرم به‌طور بسیار معنیداری افزایش یافت و با مصرف پودر رزماری نرخ آسیب کاهش یافت (p>0/01). براساس نتایج حاصل از این پژوهش، افزودن پودر گیاه رزماری به جیره خروسهای مسن بومی در شرایط تنش حرارتی، میتواند برخی صفات تولید مثلی را بهبود بخشد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of addition of Rosemary leaf powder in rooster diet on semen parameters and DNA damage under heat stress
نویسندگان [English]
- Tayebeh Omidvar 1
- Saied Mohammadzadeh 2
- mosayeb amiri 3
1 Jahade Keshavarzi Lorestan - Iran
2 Associate Professor of Animal Science Department, Faculty of Agriculture, Lorestan University,Iran
3 Lorestan University
چکیده [English]
To investigate the effect of rosemary powder on semen parameters and DNA fragmentation under heat stress, 40 native rooster aged 42 weeksand weighted of 2300 gramswere usedin a factorial experiment in randomized complete block design with two levels of temperature (normal and heat stress) and rosemary powder (0 and 7.5 gr/kg of diet) during 7 weeks. Heat stress and normal temperature was set 28±2ºC and 18-22º C,respectively.After two weeks adaptation,the semen sampleswere collected twice in a week from roosters during 5 weeks using dorso-abdominal massage method. The semen parameters including motility, viability ratio and DNA fragmentation were evaluated. The results showed that heat stress significantly reduced the progressive motility of sperm (type A) and with the addition of rosemary powder, sperm viability increased (P˂0.05). Addition of rosemary leaf powder at normal temperature decreased the DNA fragmentation ratio (P˂0.05). In roosters under heat stress,the DNA fragmentation rate of sperm increased and the damage rate decreased significantly with the feeding of rosemary powder (P˂0.01). According to the results of this study, adding rosemary powder to the diet of native old roosters under heat stress conditions can improve some reproductive traits
کلیدواژه‌ها [English]
- DNA fragmentation
- Heat stress
- Rooster
- Rosemary
- Viability
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