نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد،گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران.

2 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران.

3 استادیار، گروه علوم پایه، دانشکده دامپزشکی،دانشگاه شیراز، شیراز، ایران

چکیده

تأثیرپودر برگ رزماری روی فراسنجه­های منی و نرخ آسیب DNA تحت شرایط تنش گرمایی با استفاده از 40 قطعه خروس بومی در سن 42 هفته و وزن2300 گرمدرقالب آزمایش فاکتوریل 2×2 طرح بلوک کامل تصادفی با دو سطح درجه حرارت(طبیعی و تنش گرمایی) و دوسطح پودررزماری (بدون رزماریو 7/5 گرم پودررزماری درکیلوگرم جیره)به‌مدت هفت هفته بررسی شد.دمای سالن در شرایط طبیعی22-18و در تنش گرمایی 2±28 درجه سلسیوس تنظیم شد. بعد از دو هفته عادت‌پذیری، به‌مدت پنج هفته، هر هفته دوباراز طریق ماساژ شکمی- پشتی، از پرندگاننمونه منی گرفته شد. نمونه­های منی، از نظر فراسنجه­های تحرک، زنده­مانی و نرخ قطعه‌قطعه‌شدن DNA اسپرم ارزیابی شدند. نتایج نشان داد درجه حرارت تنش­زا کاهش معنی­داری روی حرکت پیش­رونده (نوع A)ایجاد کرد و با افزودن پودر رزماری، زنده­مانی اسپرم افزایش یافت (p>0/05). افزودن پودر رزماری دردمای طبیعی نرخ قطعه­قطعه‌شدن DNA را به‌طور معنی­داری کاهش داد. درخروس­های تحت تنش گرمایی، نرخ قطعه­قطعه‌شدن DNA اسپرم به‌طور بسیار معنی‌داری افزایش یافت و با مصرف پودر رزماری نرخ آسیب کاهش یافت (p>0/01). براساس نتایج حاصل از این پژوهش، افزودن پودر گیاه رزماری به جیره خروس­های مسن بومی در شرایط تنش حرارتی، می­تواند برخی صفات تولید مثلی را بهبود بخشد.
 

کلیدواژه‌ها

عنوان مقاله [English]

The effect of addition of Rosemary leaf powder in rooster diet on semen parameters and DNA damage under heat stress

نویسندگان [English]

  • Tayebeh Omidvar 1
  • Saied Mohammadzadeh 2
  • mosayeb amiri 3

1 Jahade Keshavarzi Lorestan - Iran

2 Associate Professor of Animal Science Department, Faculty of Agriculture, Lorestan University,Iran

3 Lorestan University

چکیده [English]

To investigate  the effect of rosemary powder on semen parameters and DNA fragmentation under heat stress, 40 native rooster aged 42 weeksand weighted of 2300 gramswere usedin a factorial experiment in randomized complete block design with two levels of temperature (normal and heat stress) and rosemary powder (0 and 7.5 gr/kg of diet) during 7 weeks. Heat stress and normal temperature was set 28±2ºC and 18-22º C,respectively.After two weeks adaptation,the semen sampleswere collected twice in a week from roosters during 5 weeks using dorso-abdominal massage method. The semen parameters including motility, viability ratio and DNA fragmentation were evaluated. The results showed that heat stress significantly reduced the progressive motility of sperm (type A) and with the addition of rosemary powder, sperm viability increased (P˂0.05). Addition of rosemary leaf powder at normal temperature decreased the DNA fragmentation ratio (P˂0.05). In roosters under heat stress,the DNA fragmentation rate of sperm increased and the damage rate decreased significantly with the feeding of rosemary powder (P˂0.01).  According to the results of this study, adding rosemary powder to the diet of native old roosters under heat stress conditions can improve some reproductive traits

کلیدواژه‌ها [English]

  • DNA fragmentation
  • Heat stress
  • Rooster
  • Rosemary
  • Viability

1. Afonso Mde O SAM CEB RDP BSB R, et al. RP and Mancini-Filho J (2013) Phenolic compounds from Rosemary (Rosmarinus officinalis L.) attenuate oxidative stress and reduce blood cholesterol concentrations in dietinduced hypercholesterolemic rats. Nutrition & metabolism, 10(1): 1-9.
2. Agarwal A and Said TMJHru (2003) Role of sperm chromatin abnormalities and DNA damage in male infertility. Human Reproduction Update, 9(4): 331-345.
3. Al-Sereiti MAbu-Amer K and Sena P (1999) Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials. Indian Journal of Experimental of Biology, 37(2): 124-30.
4. Azad MAK, Kikusato M, Maekawa T, Shirakawa H and Toyomizu M (2010). Metabolic characteristics and oxidative damage to skeletal muscle in broiler chickens exposed to chronic heat stress. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 155(3): 401-406.
5. Boonsorn T, Kongbuntad W, Narkkong NA and Aengwanich W (2010). Effects of catechin addition to extender on sperm quality and lipid peroxidation in boar semen. American-Eurasian Journal of Agricultural and Environmental Science, 7(3): 283-288.
6. Borghei-Rad SMZeinoaldini SZhandi MMoravej H and Ansari MJT (2017) Feeding rosemary leaves powder ameliorates rooster age-related subfertility. Theriogenology, 101: 35-43.
7. Cerolini SZaniboni LMaldjian A and Gliozzi TJT (2006) Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology, 66(4): 877-886.
8. Kanter MAktas CErboga MJT and health i (2013) Heat stress decreases testicular germ cell proliferation and increases apoptosis in short term: an immunohistochemical and ultrastructural study. Toxicology and industrial health, 29(2): 99-113.
9. Li YCao YWang F and Li CJAh (2014) Scrotal heat induced the Nrf2-driven antioxidant response during oxidative stress and apoptosis in the mouse testis. Acta histochemica, 116(5): 883-890.
10. Malo CGil LGonzalez NMartínez FCano RDe Blas I, et al. (2010) Anti-oxidant supplementation improves boar sperm characteristics and fertility aftercryopreservation: comparison between cysteine and rosemary (Rosmarinus officinalis). Cryobiology, 61(1): 142-147.
11. Organization WH. World health statistics 2010. World Health Organization; 2010.
12. Ramezaninejad H and Roostaei Ali Mehr MJIJoAS (2017) Storage of rooster semen in liquid form using alcoholic extract of rosemary. Iranian Journal of Animal Science, 48(1): 51-59.
13. Sakkas D and Tomlinson M (2000). Assessment of sperm competence. In: Seminars in reproductive medicine; 2000: Copyright© 2000 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New …; p. 133-140.
14. Salces-Ortiz J, Ramón M, González C, PerezGuzman MD, Garde JJ, Garcia-Alvarez O, ... and Serrano MM (2015). Differences in the ovine HSP90AA1 gene expression rates caused by two linked polymorphisms at its promoter affect rams sperm DNA fragmentation under environmental heat stress conditions. PLoSOne, 10(2), e0116360.
15. Sanches-Silva ACosta DAlbuquerque TGBuonocore GGRamos FCastilho MC, et al. (2014) Trends in the use of natural antioxidants in active food packaging: A review, 31(3): 374-395.
16. Sanocka D and Kurpisz M (2004). Reactive oxygen species and sperm cells. Reproductive biology and endocrinology, 2(1): 1-7.
17. Shafigh H, Shakeri M, Zeinoaldini S, Kohram H, Zhandi M and Moghbeli M (2016). Improving sperm cryopreservation of rooster by using rosemary alcoholic essential oil. Animal Production, 18(3): 615-624.
18. Smith R, Kaune H, Parodi D, Madariaga M, Ríos R, Morales I and Castro A (2006). Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress. Human Reproduction, 21(4): 986-993.
19. Tousson E, Bayomy MF and Ahmed AA (2018). Rosemary extract modulates fertility potential, DNA fragmentation, injury, KI67 and P53 alterations induced by etoposide in rat testes. Biomedicine & Pharmacotherapy, 98: 769-774.
20. Türk G, Çeribaşı AO, Şimşek ÜG, Çeribaşı S, Güvenç M, Kaya ŞÖ ... and Tonbak F (2016). Dietary rosemary oil alleviates heat stressinduced structural and functional damage through lipid peroxidation in the testes of growing Japanese quail. Animal reproduction science, 164: 133-143.                                                                                                                          21. Yang HW, Hwang KJ, Kwon HC, Kim HS, Choi KW and Oh KS (1998). Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos. Human reproduction (Oxford, England), 13(4): 998-1002.
22. Zadeh ZT, Shariatmadari F, Sharafi M and Torshizi MAK (2020). Amelioration effects of n-3, n-6 sources of fatty acids and rosemary leaves powder on the semen parameters, reproductive hormones, and fatty acid analysis of sperm in aged Ross broiler breeder roosters. Poultry science, 99(2): 708-718.                                                                                             23. Zanganeh Z, Zhandi M, Zare-Shahneh A, Najafi A, Nabi MM and Mohammadi-Sangcheshmeh A (2013). Does rosemary aqueous extract improve buck semen cryopreservation?. Small Ruminant Research, 114(1): 120-125.
24. Zarghi H, Golian A and Kermanshahi H (2015). The effect of rosemary hydro-alcoholic (Rosmarinus officinalis L.) extract on performance and egg quality in laying hens. Iranian Journal of animal Science, 46(1): 1-8.
25. Zhai W, Neuman SL, Latour MA and Hester P Y. (2007). The effect of dietary L-carnitine on semen traits of white leghorns. Poultry Science, 86(10): 2228-2235.