نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناسی ارشد ژنتیک و اصلاح دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران
2 دانشیار ژنتیک و اصلاح دام، گروه علوم دامی،دانشکده کشاورزی، دانشگاه زابل
3 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران
4 دانشیار ژنتیک و اصلاح دام، گروه علوم دامی و بیوانفورماتیک، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران
چکیده
هدف از این مطالعه، بررسی میزان تفرق ژنتیکی نژادهای مختلف شتر استان سیستان و بلوچستان با استفاده از نشانگرهای ریزماهوارهای انجام شد. بدین منظور از تعداد 70 نفرشتر از دو نژاد جمازه و بلوچی به‌صورت تصادفی انتخاب و از آن‌ها از ورید وداجی در لولههای هپاررینهخون‌گیری شد. DNA در نمونههای خون به‌روش نمکی-دترجنت استخراج شد.تعداد پنج نشانگر ریزماهوارهای از ژنوم شتر انتخاب و با آغازگرهای اختصاصی تکثیر و الگوهای باندی بر روی ژل آگارز پنج درصد نمایانسازی شدند. ساختارهای ژنتیکی و جمعیتی با نرم‌افزارPOPGENE نسخه 1/31 تجزیه و تحلیل شدند. تعداد آللها در پنج جایگاه نشانگری در دو نژاد جمازه و بلوچی در دامنه یک تا شش آلل متغیر بودند. تعداد آللها در پنج نشانگر CVRLO6، VOLP32، LCV66، WYLLO و CMS17 در نژاد جمازه به‌ترتیب برابر با سه، دو، سه، چهار و یک آلل و در نژاد بلوچی به‌ترتیب برابر با دو، چهار، دو، پنج و یک آلل بودند. دامنه هتروزیگوسیتی مشاهده‌شده در جایگاههای مورد مطالعه در دو نژاد جمازه و بلوچی در محدوده 0/29تا 0/52 قرار داشت.نتایجتجزیه آماره FST نشان داد که دو نژاد شباهت بیش از ۹۶ درصد و میزان تفرق بین آن‌ها سه درصد است. نتایج این پژوهش بیانگر میزان چندشکلی پایین در جایگاههای مورد مطالعه بود و جهت حفظ نژادها و تطابقپذیری بیش‌تر به شرایط محیطی نیازمند توجه بیش‌تر و کنترل آمیزشها میباشد.
کلیدواژه‌ها
عنوان مقاله [English]
Evaluation of genetic differentiation of different camel breeds in Sistan and Baluchestan province using microsatellite markers
نویسندگان [English]
- Arezoo Tooghi 1
- Gholam Dashab 2
- Ali Maghsoudi 3
- Mohammad Rokouei 4
1 . M.Sc. Animal Breeding and Genetic, Department of Animal Science, College of Agriculture, University of Zabol, Zabol, Iran.
2 Associate Professor of Animal Breeding and Genetic, Department of Animal Science, University of Zabol, Zabol, Iran
3 Associate Professor of Animal Breeding and Genetic, Department of Animal Science, College of Agriculture, University of Zabol, Zabol, Iran.
4 . Associate Professor of Animal Breeding and Genetic, Department of Animal Science and Bioinformatic, College of Agriculture, University of Zabol, Zabol, Iran.
چکیده [English]
The aim of this study was to investigate the genetic diversity of different camel breeds in Sistan and Baluchestan province using microsatellite markers. For this purpose, 70 camels were randomly selected from Jamazeh and Baluchi breeds. Jugular vein blood sampling was performed in the tubes containing 0.5% EDTA. The extraction of DNA was performed by salt-detergent method. Five microsatellite markers were selected from the camel genome project with specific primers and banding patterns on 5% agarose gel. Genetic and population structures were analyzed using POPGENE v1.31 software. The number of alleles in five marker positions in Jamazeh and Baluchi breeds ranged from one to six alleles. The number of alleles in five markers CVRLO6, VOLP32, LCV66, WYLLO and CMS17 in Jamazeh are equal to three, two, three, four and one alleles, respectively, and in Baluchi breed with two, four, two, five and one alleles, respectively. According to the results, the range of heterozygosity observed in the studied sites in Jamazeh and Baluchi breeds was in the range from 0.29 to 0.52. The results of FST analysis showed that the two populations were more than 96% similar and the difference between them was 3%. The results of the presentresearchindicated a low rate of polymorphism in the studied sites and in order to preserve breeds and be more adaptable to environmental conditions, it needs more attention and control of inbreeding.
کلیدواژه‌ها [English]
- Baluchi camel
- Gene flow
- Heterozygosity
- Jamazeh camel
- Polymorphic information content
- Ghasemi Meymandi M, Mohammadabadi MR and Esmailizadeh A (2015) Genetic variation of camels in the North of Kerman province using microsatellite markers. Animal Production Research, 4(1): 35-45. (In Persian)
- Ghasemi Meymandi M, Mohammadabadi MR, Esmailizadeh A and Montazeri M (2015) Assigning individuals to the camel populations of North of Kerman province using Microsatellite markers. Novin Genetic, 11(3): 325-329. (In Persian)
- Glasko V (2003) An attempt at understanding the genetic basis of domestication. Animal Science, 2: 109-120.
- Han Y, Jedoschenko D, Hue G, Reiner G and Geldermann H (2000) Screening and analysis of new microsatellite loci in camels. University of Hohenheim, Stuttgart, Germany.
- Jianlin H, Mburu D, Ochieng J, Kaufmann B, Rege J and Hanotte O (2000) Ampplication of new world camelidae microsattelite primers for amplication of polymorphic loci in old world camelides. Animal Genettics 31: 404-419.
- Jianlin H, Ochieng J, Lkhagva B and Hanotte O (2004)Genetic diversity and relationship of domestic Bactriancamels (Camelus bactrianus) in China and Mongolia.Journal of Camel Practice and Research, 12: 97-99.
- Kashongwe OB, Mwangi LW, Bebe BO, Matofari JW and Huelsebusch C (2017) Influence of on-farm feed formulations and hygiene interventions on milk yield and quality in smallholder dairy farms in Kenya. International Journal of Agricultural Extension, 5(2): 11-17.
- King RC, Stansfield WR and Mullign PK (2006) Dictionary of Genetics. 7th Oxford University Press. p16; p174.
- Liu K and Muse SV (2005) PowerMarker: An integrated analysis environment for genetic marker analysis. Bioinformatics, 21: 2128-2129.
- Mahmoud AH, Alshaikh MA, Aljumaah RS and Mohammed OB (2012) Genetic variability of camel (Camelusdromedarius) populations in Saudi Arabia based on microsatellites analysis. African Journal of Biotechnology, 11(51): 1173-11180
- Mate´ M, Bustamante A, Giovambattista G, de Lamo D, von Thungen J, Zambelli A and Vidal-Rioja L (2005) Genetic diversity and differentiation of guanaco populations from Argentina inferred from microsatellite data. Animal Genetics 36: 316-321.
- Mohammadabadi MR, Ghasemi Meymandi M and Montazeri M (2018) The Study of Genetic Diversity of Camels in North of Kerman Province Using F Statistics. Breeding and Improvement of Livestock, 1(2): 1-13. (In Persian)
- Mohammadi Y, Ghanbari S, Dehrebi Koohi H, Sataei Mokhtari M and Esmailzadeh A (2011) Study of genetic similarity based on pedigree data and a limited number of molecular microsatellite markers. Novin Genetic, 6(2): 71-82. (In Persian)
- Nobahari H, Bahari A and Gazanfari S (2021) Molecular diversity and phylogenetic analysis of Turkmen camel and different species of camels based on CYTB gene sequence. Iranian Journal of Animal Science Research, In Print. (In Persian)
- Sadeghi M, Mohammadi H, Moradi Shahrbabak M and Moradi Shahrbabak H (2011) Comparison of the efficiency of the buffer-detergent extraction and standard salting-out methods for DNA extraction from blood and semen samples. Genetics in the 3RD Millennium, 8(4): 2155-2161. (In Persian)
- Salehi M and Gharahdaghi AA (2013) Camel production potential and recent research in Iran. Animal Science Research Institute, Tehran. (In Persian)
- Schulz U, Tupac-Yupanqui I, Martínez A, Méndez S, Vicente Delgado J, Gómez M, Dunner S and Cañón J (2010) The Canarian camel: a traditional dromedary population. Diversity 2: 561-571.
- Shahkarami S,Afraz F,Sayed Mirhosini Z,Banabazi MH,Asadzadeh N,Asadi N,Hemmati B, Ghanbari A and Razavi K (2012) Genetic diversity in Tranian Bactrian camels (Camelus Batrianus) using microsatellite markers. Modern Genetics Journal (MGJ) 7(3): 249-258. (In Persian)
- Simianer H (2005) Decision making in livestock conservation. Ecological Economics 53: 559-572.
- Spencer PBS and Woolnough AP (2010) Assessment and genetic characterization of Australian camels using microsatellite polymorphisms. Livestock Science 129: 241-245.
- Spencer PBS, Wilson KJ and Tinson A (2010) Parentage testing of racing camels (Camelus dromedaries) using microsattelite DNA typing. Animal Genetics 41: 662-665.
- Tadesse Y, Costa V, Gebremariam Z, Urgae M, Tilahun S, Kebede K and Beja-Pereira A (2019) Genetic Variability and Relationship of Camel (Camelus dromedarius) Populations in Ethiopia as Evidenced by Microsatellites Analysis. Ethiopian Journal of Agricultural Sciences 29(1): 19-37.
- Vijh RK, Tantia MS, Mishra B and Bharani Kumar ST (2007) Genetic diversity and differentiation on Dromedarian camel of India. Animal Biotechnology 18: 81-90.
- Yeh FC, Boyle T and Yang RC (1999) PopGene: Microsoft window-based freeware for population genetic analysis, version 1.31.
)