نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی دام، گروه علوم دامی، دانشگاه کشاورزی و منابع طبیعی خوزستان

2 دانشیار گروه علوم دامی دانشگاه علوم کشاورزی و منابع طبیعی خوزستان.

3 دانشیار گروه علوم دامی، دانشگاه کشاورزی و منابع طبیعی خوزستان

چکیده

هدف از این پژوهش، بررسی تاثیر عصاره مارچوبه بر کیفیت منی قوچ عربی در زمانهای مختلف ذخیره‌سازی به حالت مایع در دمای چهار درجه سانتی‌گراد‌ بود. اسپرم‌گیری از 10راس قوچ نژاد عربی با متوسط سن 2/5 سال بطور هفتگی (هر هفته یکبار) به مدت شش هفته انجام شد. پس از افزودن سطوح صفر، 1/5، سه و 4/5 درصد عصاره مارچوبه به رقیق‌کننده منی، فراسنجه‌های کیفی اسپرم در زمان‌های صفر، 24، 48 ،72 ساعت از نگهداری تیمارها به حالت مایع مورد ارزیابی قرار گرفتند. در زمان صفر، زنده‌مانی اسپرم در رقیق کننده حاوی سه درصد عصاره مارچوبه بیشتر از رقیق کننده حاوی سطوح صفر و 4/5 درصد عصاره بود (P <0.05). پس از 24 ساعت ذخیره‌سازی منی، فراسنجه‌های تحرک کل، جنبایی پیشرونده، سلامت غشای پلاسمای اسپرم و زنده‌مانی اسپرم، در رقیق کننده حاوی 1/5 و سه درصد عصاره بالاتر بودند (P <0.05). فراسنجه‌های کیفی اسپرم در 48 ساعت پس از اسپرم‌گیری و ذخیره‌سازی در رقیق کننده حاوی سه درصد عصاره معنی‌دار و عملکرد بهتری داشتند (P <0.05). بالاترین میزان سلامت غشایی پلاسمایی، جنبایی پیش‌رونده و تحرک کل اسپرم در 72 ساعت پس از ذخیره‌سازی اسپرم، در رقیق کننده حاوی سه درصد عصاره مشاهده شد (P <0.05). بر اساس نتایج حاصل در این مطالعه استفاده از عصاره مارچوبه در رقیق‌کننده به میزان سه درصد کیفیت اسپرم قوچ‌های عربی پس از شرایط سردسازی را بهبود می‌بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of Asparagus officinalis extract on Arabi ram semen quality during storage in liquid condition at 4 ◦C

نویسندگان [English]

  • Abuzar gholampour 1
  • Khalil Mirzadeh 2
  • Saleh TabatabaeiVakili 3

1 M.Sc. Student of Animal Physiology, Department of Animal Science, Ramin Agriculture and Natural Resources University of Khuzestan

2 Department of Animal Science, Agricultural Sciences and Natural Resources University of Khuzestan

3 Associate Professor, Department of Animal Science, Ramin Agriculture and Natural Resources University of Khuzestan

چکیده [English]

The study aimed to investigate the effect of asparagus extract on quality semen of Arabian rams at different storage times at a temperature of 4
degrees Celsius. Sperm collection was performed from 10 Arab rams with an average age of 2.5 years per week (once a week) for six weeks. After
adding 0, 1.5, 3 and 4.5% asparagus extract to the semen diluent, sperm quality parameters were evaluated at 0, 24, 48, and 72 hours after storage in
liquid condition. At zero hour, sperm viability in diluent had 3% asparagus extract was higher than diluent containing 0 and 4.5% extract (P<0.05).
After 24 hours of semen storage, the parameters of total motility, progressive motility, plasma membrane integrity and viability were higher in sperm
diluent containing 1.5 and 3% of the extract (P<0.05). Qualitative parameters of sperm in 48 hours after sperm collection and storage in diluent
contain 3% of extract had significant and better performance (P<0.05). The highest rate of plasma membrane integrity, progressive motility and total
sperm motility was observed in diluent containing 3% extract in 72 hours after sperm storage (P<0.05). According to the results of this study, the use
of asparagus extract in the diluent at the rate of 3% improves the sperm quality of Arabian rams after cooling conditions.

کلیدواژه‌ها [English]

  • Arabic ram
  • Asparagus
  • Motility
  • Sperm
  • storage
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