The effects of different sources of copper supplementation on growth performance, some blood biochemical and immune parameters, microbial population of small intestine and meat quality were investigated with using of 240 male broiler chicks in a completely randomized design with 5 treatments and 4 replicates from 1 to 42 days. Treatments consisted of control treatment (basal diet without copper supplement) and four diets containing 16 mg/kg of copper-glycine, copper-sulfate, copper-nanochelate and copper nano-glycinec. The results showed that the use of different sources of copper had no effect on growth performance, some blood biochemical parameters and percentage of heterophil, lymphocyte, white blood cell, antibody titer against Newcastle disease, cholesterol, triglycerides and water holding capacity and pH of meat of broilers. Gastrointestinal relative weight in birds fed with diet containing copper-glycine was lower than the birds receiving copper-nanochelate (P<0.05). The total microbial population of ileum in birds fed diets without copper and copper-sulfate was higher than birds receiving diet containing copper-glycine (P<0.01). Meat malondialdehyde concentration of birds fed with diets containing copper-glycine and copper nano-glycine after 6 day of storage at refrigerator was lower than other birds (P<0.01). According to the results, the use of 16 mg/kg of copper from organic, mineral and nano sources in the diet has no effect on growth performance, immune system and ileal microbiota in broilers, but the use of copper-glycine and copper nano-glycine supplements in the diet reduces the oxidation of meat.