نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دکترای عمومی دامپزشکی، دانشکده دامپزشکی، دانشگاه لرستان، خرم آباد، ایران.

2 استاد،عضو هیآت علمی دانشگاه لرستان، تخصص: مامایی و بیماری‌های تولیدمثل دام/ ارزیابی اسپرم در حیوانات اهلی و موش‌ها/ سونوگرافی حیوانات

3 استادیار،گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه لرستان، خرم آباد، ایران

4 استادیار،گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، ایران

چکیده

هدف ازانجام این پژوهش، بررسی تغییرات برخی فراسنجه‌های بیوشیمیایی خون درمیش نژاد لری بختیاری قبل از جفت‌گیری و ماه‌های مختلف آبستنی و تاثیر تعداد جنین براین فراسنجه‌ها بود. بدین منظور 20 رأس میش نژاد لری بختیاری انتخاب و با قوچ‌های بارور جفت‌گیری داده شدند. یک ماه قبل ازجفت‌گیری وهمچنین در ماه‌های اول تا پنجم آبستنی نمونه خون از میش‌ها اخذ و فراسنجه‌های بیوشیمیایی و آنزیمی شامل کلسیم، فسفر، منیزیم، اوره، کراتینین، بیلی‌روبین تام، AST، ALP و GGT اندازه‌گیری شدند. غلظت کلسیم و فعالیت AST در زمان‌های مورد مطالعه ثابت بوده ولی در ماه آخر آبستنی افزایش یافت (p<0/05). غلظت منیزیم در ماه دوم و ماه آخر آبستنی افزایش (p<0/05) و غلظت فسفر در دوره‌ی آبستنی نسبت به ماه پیش از جفت‌گیری کاهش یافت (p<0/05). غلظت کراتینین در ماه دوم و سوم آبستنی نسبت به دیگر ماه‌های آبستنی کاهش (p<0/05) و غلظت بیلی‌روبین تام در ماه چهارم و غلظت اوره در نیمه دوم آبستنی افزایش پیدا کرد (p<0/05). فعالیت GGT تقریبا ثابت بود ولی در ماه چهارم آبستنی کاهش (p<0/05) و فعالیت ALP طی دوره آبستنی به طور کلی روند افزایشی داشت (p<0/05). میانگین غلظت فراسنجه‌های مورد مطالعه در میش‌های تک‌قلو آبستن تفاوت معنی‌داری با دوقلو نداشت (p<0/05). بر اساس نتایج این مطالعه تغییرات بیوشیمیایی خون در میش‌های نژاد لری بختیاری می‌تواند برای ارزیابی وضعیت فیزیولوژیکی آن‌ها در دوران آبستنی استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation of some blood biochemical parameters changes in pregnant Lori-Bakhtiari ewes during different months of pregnancy

نویسندگان [English]

  • aref kord 1
  • Arash Kheradmand 2
  • Alireza Rocky 3
  • Majid Khaldari 4

1 clinicin

2 Lorestan University faculty member, specialty: Midwifery and reproductive diseases of livestock / Sperm evaluation in domestic animals and mice / Ultrasound of domestic animals / Mastitis / The effect of antioxidants on reproduction

3 Department of Clinical Pathology, Lorestan University, Khoramabad, Iran

4 Department of Animal Science, Faculty of Agriculture, Lorestan university. Iran

چکیده [English]

The aim of this study was to investigate the changes of some blood biochemical parameters in Lori-Bakhtiari ewes during different months
of pregnancy and the influence of fetus numbers on these parameters. For this purpose, 20 ewes of Lori Bakhtiari breed were selected and
mated with fertile rams. One month before mating and in the first to fifth months of pregnancy blood samples were collected from ewes and
biochemical and enzymatic parameters including calcium, phosphorus, magnesium, urea, creatinine, total bilirubin, AST, ALP and GGT
were measured. The concentration of calcium and AST activity were constant at the times of the study, but they increased in the last month
of pregnancy (P<0.05). Magnesium concentration increased in second month of pregnancy (P<0.05) and phosphorus concentration was
decreased during pregnancy compared to the month before mating (P<0.05). Creatinine concentration decreased in the second and third
months of pregnancy compared to other months of pregnancy (P<0.05). Total bilirubin and urea concentrations increased during fourth
month and the second half of pregnancy, respectively (P<0.05). GGT activity was almost stable during study period but decreased in the
fourth month of pregnancy (P<0.05), and ALP enzyme activity showed increasing trend during pregnancy period (P<0.05). The mean
concentration of the studied parameters in singleton ewes was not significantly different from twins (P <0.05). Based on the results of this
study, blood biochemical changes in Lori Bakhtiari ewes can be used to assess their physiological status during pregnancy.

کلیدواژه‌ها [English]

  • bilirubin
  • Blood changes
  • electrolyte
  • enzyme
  • Lori-Bakhtiary sheep
1. Alonso A, De Teresa R, Garcia M, Gonzalez J
and Vallejo M (1997) The effects of age and
reproductive status on serum and blood
parameters in Merino breed sheep. J Vet Med
A, 44(1‐10): 223-231.
2. Al-Sobaiyl K (2010) Effect of breeding season
and pregnancy status on serum progesterone,
sodium, potassium, copper and iron of estrous
synchronized Aradi goat does. Saudi J Biol
Sci, 17(3): 259-263.
3. Antunović Z, Senčić Đ, Šperanda M and Liker
B (2002) Influence of the season and the
reproductive status of ewes on blood
parameters. Small Rumin Res, 45(1): 39-44.
4. Antunović Z, Šperanda M and Steiner Z (2004)
The influence of age and the reproductive
status to the blood indicators of the ewes. Arch
Anim Breed, 47(3): 265-273.
5. Atiabi N (2010) Veterinary clinical pathology:
laboratory methods. Tehran University Press,
Tehran, Iran p: 170-174, 203-204, 234-238.
(In Persian).
6. Balıkcı E, Yıldız A and Gürdoğan F (2007)
Blood metabolite concentrations during
pregnancy and postpartum in Akkaraman
ewes. Small Rumin Res, 67(2-3): 247-251.
7. El-Sherif M and Assad F (2001) Changes in
some blood constituents of Barki ewes during
pregnancy and lactation under semi arid
conditions. Small Ruminant Research. 40(3):
p. 269-27.
8. El-Tarabany AA (2012) Physiological Changes
in Ewes Conceived Single or Twins Fetuses
Related with Survivability of Lambs. Arab J
Nucl Sci and Appl, 45(3): 223-235.
9. Gerber H, Martig J and Straub R (1973)
Enzymuntersuchungen im Serum von
Grosstieren im Hinblick auf Diagnose und
Prognose. Tierärztl. Praxis, 1: 5-18.
10. Gürgöze SY, Zonturlu AK, Özyurtlu N and
Icen H (2009) Investigation of some
biochemical parameters and mineral substance
during pregnancy and postpartum period in
Awassi ewes. Kafkas Univ Vet Fak Derg
15(6): 957-963.
11. Harvey JW, et al. (2005) Clinical biochemistry
of pregnant and nursing mares. Veterinary
clinical pathology. 34(3): p. 248-254.
12. Iriadam M (2007) Variation in certain
hematological and biochemical parameters
during the peri-partum period in Kilis does.
Small Rumin Res, 73(1): 54-57.13. Khatun A, Wani G, Bhat J, Choudhury A and
Khan M (2011) Biochemical indices in sheep
during different stages of pregnancy. Asian J
Anim Vet Adv, 6(2): 175-181.
14. Moghaddam G and Hassanpour A (2008)
Comparison of blood serum glucose, beta
hydroxybutyric acid, blood urea nitrogen and
calcium concentrations in pregnant and lambed
ewes. J Anim Vet Adv, 7(3): 308-311.
(In Persian).
15. Prior RL and Christenson RK (1978) Insulin
and glucose effects on glucose metabolism in
pregnant and nonpregnant ewes. Journal of
Animal Science, 46(1), 201-210.
16. Rowlands GJ (1980) A review of variations in
the concentrations of metabolites in the blood
of beef and dairy cattle associated with
physiology, nutrition and disease, with
particular reference to the interpretation of
metabolic profiles. World Rev Nutr Diet, 35:
172-235.
17. Sansom BF, Bunch KJ and Dew SM (1982)
Changes in Plasma Calcium, Magnesium,
Phosphorus and Hydroxyproline
Concentrations in Ewes from Twelve Weeks
before until three weeks after Lambing. Brit
Vet J, 138(5): 393-401.
18. Shetaewi MM and Ross TT (1991) Effects of
concentrate supplementation and lasalocid on
serum chemistry and hormone profiles in
Rambouillet ewes. Small Rumin Res, 4(4):
365-377.
19. Sigurdsson H (1988) The effects of flock,
number of fetuses and age on some
biochemical blood constituents in ewes in late
pregnancy under field conditions.
Transboundary and Emerging Diseases. 35(1‐
10): p. 417-423.
20. Tanritanir P, Dede S, and Ceylan E (2009)
Changes in some macro minerals and
biochemical parameters in female healthy Siirt
hair goats before and after parturation. Journal
of Animal and Veterinary Advances. 8(3): p.
530-533.
21. Waziri MA, Ribadu AY and Sivachelvan N
(2010) Changes in the serum proteins,
hematological and some serum biochemical
profiles in the gestation period in the Sahel
goats. Vet Arhiv, 80(2): 215-224.
22. Yildiz H, Kaygusuzoglu E and Yildiz S (2006)
Investigation of Relationship Between Serum
Progesterone, Vitamin A, ß-Carotene and
Magnesium Levels During Pregnancy and at
Postpartum Period. J Health Sci, 20: 345-349.
23. Yokus B, Cakir DU, Kanay Z, Gulten T and
Uysal E (2006). Effects of Seasonal and
Physiological Variations on the Serum
Chemistry, Vitamins and Thyroid Hormone
Concentrations in Sheep. J Vet Med A, 53(6):
271-276.