نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دوره دکترا /گروه علوم دامی،دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران.

2 استاد/گروه علوم دامی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران.

چکیده

مطالعه حاضر با هدف بررسی اثر لاکتوباسیلوس فرمنتوم 92069 (Lactobacillus fermentum) جداسازی شده از ماست بر تخمیر شیمیایی، میکروبی و پایداری هوازی سیلاژ ذرت با رطوبت بالا انجام شد. به منظور انجام این آزمایش، لاکتوباسیلوس فرمنتوم پس از تکثیر و تعیین غلظت جهت تهیه تیمارهای آزمایشی به علوفه ذرت افزوده شد. تیمارهای آزمایشی شامل غلظت های صفر (شاهد، LF0)، cfu 106 ×1 (LF1) و cfu106 × 2 (LF2) به ازای هر گرم علوفه تازه در سه تکرار تهیه و سیلوهای آزمایشگاهی به مدت 90 روز نگهداری شدند. نتایج آزمایش نشان داد که ترکیبات شیمیایی سیلاژها شامل ماده خشک، کربوهیدرات های محلول در آب، پروتئین خام و الیاف نامحلول در شوینده خنثی و شوینده اسیدی تحت تاثیر افزودن باکتری قرار نگرفت pH سیلاژ مربوط به تیمار LF2 کمتر از تیمار شاهد بود (0/05>P). غلظت اسید لاکتیک در سیلاژهای LF1 و LF2 بالاتر از تیمار شاهد بود (0/05>P). غلظت اسید استیک و جمعیت کپک سیلاژ LF2 به ترتیب بیشتر و کمتر از سیلاژهای دیگر بود (0/05>P). جمعیت باکتری های اسید لاکتیک و مخمر سیلاژها تحت تاثیر افزودن باکتری قرار نگرفت. پایداری هوازی سیلاژ LF0 و LF1 در مقایسه باسیلاژ LF2 کاهش یافت (0/05>P). در مرحله هوازی مقدار pH سیلاژ LF2 کمتر از دیگر سیلاژها بود (0/050>P) اما در جمعیت مخمر سیلاژها در این مرحله اختلاف معنی داری مشاهده . نتایج این مطالعه نشان داد که لاکتوباسیلوس فرمنتوم 92069 قابلیت استفاده به عنوان تلقیح کننده سیلاژ را دارد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Lactobacillus fermentum isolated from yogurt on fermentation quality and aerobic stability of high moisture corn silage

نویسندگان [English]

  • Leila Taherabadi 1
  • Farokh Kafilzadeh 2

1 Ph.D. Candidate. Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran.

2 Professor, Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran.

چکیده [English]

The aim of this study was to investigate the effect of Lactobacillus fermentum 92069 (LF) isolated from yogurt on chemical and microbial
fermentation and aerobic stability of high moisture corn silage. After propagation and concentration determination LF was used to prepare
experimental treatments with concentrations of zero (control, LF0), 1×106 cfu/g fresh forage (LF1) and 2×106 cfu/g fresh forage (LF2). Three replicates
of each treatment were stored in laboratory silos for 90 days. The results showed that the chemical composition of silages (DM, NDF, ADF, CP,
WSC) was not affected by addition of LF. LF2 had a significant lower pH compared to the control (P<0.05). LF1 and LF2 silages showed a higher
concentration of lactic acid (P<0.05). Concentration of acetic acid increased and mold population decreased in LF2 compared to the other silages
(P<0.05). There was no significant difference between population of lactic acid bacteria and yeast in silages. The aerobic stability of LF0 and LF1
silages decreased significantly compared to LF2 (P<0.05). During the aerobic stage after opening the silos, LF2 silage had the lowest pH (P<0.05).
However, yeast population of silages during the aerobic stage was not affected by treatment. The results of this study showed that Lactobacillus
fermentum 92069 has the potential to be used as a silage inoculant.

کلیدواژه‌ها [English]

  • aerobic stability
  • corn silage
  • Fermentation
  • Lactobacillus fermentum
  • Microbial population
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