نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم دامی، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

2 استاد، گروه علوم دامی، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 دانشیار، گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه کشاورزی و منابع طبیعی ساری، ساری، ایران.

چکیده

در این پژوهش، اثر تغذیه کوآنزیم  Q10و اسیدهای چرب امگا3 بر بافت‌شناسی و بیان نسبی ژن­های TGF-b4 و StAR­ در بیضه خروس­های نژاد هوبارد بررسی شد. تعداد 48 خروس در سن 50 هفتگی به‌صورت تصادفی به چهار گروه مساوی تقسیم شدند و جیره پایه به اضافه سطوحی از کوآنزیم Q10 و امگا3 شامل صفر، 30 گرم در کیلوگرم امگا3 (سالومگا)، 400 میلی­گرم در کیلوگرم کوآنزیم Q10 و مخلوط کوآنزیم Q10 و امگا3 (30+400) در روز به‌مدت هشت هفته متوالی دریافت کردند. در پایان آزمایش خروس­ها کشتار و بیضه­ها به دقت خارج شدند. سپس دو نمونه از یک بیضه از هر تکرار برداشته شد، یک نمونه برای بررسی بافت‌شناسی به درون محلول فرمالین 10 درصد و یک نمونه جهت بررسی بیان نسبی ژن به درون ازت مایع منتقل شد. نتایج نشان داد که قطر لوله­های اسپرم‌ساز در گروه­های آزمایشی نسبت به گروه شاهد به‌طور معنی­داری افزایش یافت (p<0/05)، ولی تعداد سلول­های سرتولی و لایدیگ تفاوت معنی­داری نداشت. بیان نسبی ژن  TGF-b4 در سه گروه آزمایش نسبت به گروه شاهد کاهش یافت (p<0/05). اما بیان نسبی ژن StAR در پرندگانی که با استفاده از مخلوطی از کوآنزیم Q10 و امگا3 (سالومگا) تغذیه شدند، به‌طور معنی­داری افزایش یافت (p<0/05). براساس نتایج این مطالعه اضافه‌نمودن مخلوطی از کوآنزیم Q10 و اسیدهای چرب امگا3 در خوراک خروس­ها، فراسنجه­های بافت‌شناسی و بیان ژن‌های مرتیط با اسپرم‌سازی را بهبود می­بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

Changes of testis histology parameters and relative expression of TGF-4 & StAR genes in roosters fed CoQ10 and omega 3 fatty acids

نویسندگان [English]

  • hamid reza salari 1
  • Yousef Jafari Ahangari 2
  • Zarbakht Ansari Pirsaraie 3

1 , Animal Science Department, College of Agriculture &amp;amp;amp; Natural Resources, University of Gorgan, Iran

2 Animal Science faculty, Gorgan university agricultural sciences &amp; natural resources

3 Sari university of agricultural sciences &amp; natural resources

چکیده [English]

In this research, the effect of feeding coQ10 and omega 3 fatty acids on testicular histology and relative expression of TGF-b4 & StAR genes in Hubbard roosters was investigated. Forty-eight roosters, at fifty weeks age, were randomly divided into four groups and were subjected to the basal diet supplemented with  omega-3 (30gr), coQ10 (400mg) and  omega-3+ coQ10 (30gr+400mg) per day for eight successive weeks. At the end of the trial, all birds were killed and two samples were collected from the same testicle one of which was processed for histology, whereas another was snap-frozen in liquid nitrogen to assess relative gene expression. Results showed that seminiferous tubule diameters of experimental groups were significantly improved compared to the control group (P<0.05). However, leydig cell numbers and sertoli cells were not significantly affected. The relative expression of TGF-b4 genes were significantly decreased in three groups compared to control (P<0.05). Birds fed coQ10 and omega-3 had significantly higher StAR transcript level compared to other groups (P<0.05). Results of the present study showed that addition of a mixture of coQ10 and omega-3 fatty acids to rooster`s feed, lead to improvement in testicular histology and genes expression related to spermatogenesis.

کلیدواژه‌ها [English]

  • Omega 3 fatty acids
  • Fertility
  • Leydig cells
  • seminiferous tubule diameters
  • Sertoli cells
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