نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش اموخته کارشناسی ارشد، گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران.

2 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران.

چکیده

 پژوهش حاضر به‌منظور بررسی اثر تریپتوفان، ملاتونین و دی‌متیل گلایسین بر عملکرد رشد و کیفیت گوشت بلدرچین­های ژاپنی تغذیه‌شده با جیره‌های آلوده‌شده با سم آفلاتوکسین B1 انجام شد. در این آزمایش تعداد 680 قطعه بلدرچین ژاپنی از سن هفت‌ تا 35 روزگی در 17 تیمار و چهار تکرار و در هر تکرار 10 قطعه بلدرچین ژاپنی با استفاده از  طرح مرکب مرکزی مورداستفاده قرار گرفتند. عملکرد پرندگان به‌صورت هفتگی محاسبه و در انتهای آزمایش میزان مالون‌دی‌آلدهید گوشت اندازه­ گیری شد. نتایج نشان داد که تریپتوفان سبب افزایش معنی­دار وزن بدن شد (p<0/05) و ملاتونین تأثیر افزایشی بر مصرف خوراک داشت (p<0/05). اثر اصلی تریپتوفان و هم‌چنین اثر متقابل تریپتوفان و دی‌متیل گلایسین میزان مالون‌دی‌آلدهید گوشت را کاهش داد (p<0/05). نتایج مطالعه حاضر نشان داد تریپتوفان و دهنده­های گروه متیل دارای ظرفیت آنتی­اکسیدانی مناسبی برای کاهش مسمومیت ناشی از آفلاتوکسین B1 بر عملکرد رشد و میزان مالون‌دی‌آلدهید گوشت هستند و استفاده از این مکمل­ها در جیره غذایی بلدرچین ژاپنی مطلوب به نظر می­رسد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of different level of Tryptophan and methyl group donors on the performance of Japanese quail fed diets contaminated with aflatoxin B1

نویسندگان [English]

  • Vahideh Sabaghi-Darmiyan 1
  • Mehran Mehri 2
  • Farzad Bagherzadeh Kasmani 2
  • Mohammad Rokouei 2

1 دانشگاه زابل دانشکده کشاورزی گروه علوم دامی

2 University of Zabol

چکیده [English]

The experiment was carried out  to investigate the effect of tryptophan, melatonin and dimethylglycine on the growth performance and meat quality of Japanese quails fed diets contaminated with aflatoxin B1 toxin. In this experiment, 680 Japanese quail from 7 to 35 days of age were allotted to 17 treatments and 4 replicates (10 birds per replication), and  trial performed using a central composite design. The performance of birds was calculated on a weekly basis and at the end of the experiment; and the amount of malondialdehyde in meat was determined. Results showed that tryptophan increase weight gain and melatonin had an increased effect on feed intake (P < 0.05). The effect of tryptophan and as well as the interaction of tryptophan and dimethylglycine reduced the amount of malondialdehyde in meat (P < 0.05). The results obtained from this research state that dietary supplementation with tryptophan and methyl group donors have good antioxidant capacity to reduce aflatoxin B1 poisoning on growth performance and malondialdehyde content of meat, and the use of these supplements in the diet of Japanese quails seems desirable.

کلیدواژه‌ها [English]

  • Aflatoxin B1
  • Central composite design
  • Dimethylglycine
  • Japanese quail
  • Malondialdehyde
  • Melatonin
  • Tryptophan
  1. Bagherzadeh-Kasmani F and Mehri M (2015) Effects of a multi-strain probiotics against aflatoxicosis in growing Japanese quails. Livestock Science, 177: 110-116.
  2. Bai M, Liu H, Xu K, Oso AO, Wu X, Liu G, Tossou MC, Al-Dhabi NA, Duraipandiyan V, Xi Q and Yin Y (2017) A review of the immunomodulatory role of dietary tryptophan in livestock and poultry. Amino Acids, 49(1): 67-74.
  3. Bravo R, Matito S, Cubero J, Paredes SD, Franco L, Rivero M, Rodríguez AB and Barriga C (2013) Tryptophan-enriched cereal intake improves nocturnal sleep, melatonin, serotonin, and total antioxidant capacity levels and mood in elderly humans. Age, 35(4): 1277-85.
  4. Chen J, Chen K, Yuan S, Peng X, Fang J, Wang F, Cui H, Chen Z, Yuan J and Geng Y (2016) Effects of aflatoxin B1 on oxidative stress markers and apoptosis of spleens in broilers. Toxicology and Industrial Health, 32(2): 278-84.
  5. Chen X, Du W and Liu D (2008) Response surface optimization of biocatalytic biodiesel production with acid oil. Biochemical Engineering Journal, 40(3): 423-9.
  6. Chen X, Naehrer K and Applegate TJ (2016) Interactive effects of dietary protein concentration and aflatoxin B1 on performance, nutrient digestibility, and gut health in broiler chicks. Poultry Science, 95(6): 1312-25.
  7. Cupp MJ and Tracy TS (2003) Dimethylglycine (N, N-Dimethylglycine). Dietary supplements: Springer. pp. 149-60.
  8. Essiz DI, Altintas L and Das YK (2006) Effects of aflatoxin and various adsorbents on plasma malondialdehyde levels in quails. Bulletin of the Veterinary Institute in Pulawy, 50(4): 585-590.
  9. Faix Š, Faixová Z, Plachá I and Koppel J (2009) Effect of Cinnamomum zeylanicum essential oil on antioxidative status in broiler chickens. Acta Veterinaria Brno, 78(3): 411-7.
  10. Hussein MR, Ahmed OG, Hassan AF and Ahmed MA (2007) Intake of melatonin is associated with amelioration of physiological changes, both metabolic and morphological pathologies associated with obesity: an animal model. International Journal of Experimental Pathology, 88(1): 19-29.
  11. Ifeanyichukwu CE, Joseph OA, Mohammed UK and Aliyu M (2017) Ameliorative effects of betaine and ascorbic acid on erythrocyte osmotic fragility and malondialdehyde concentrations in broiler chickens during the hot-dry season. Journal of Applied Animal Research, 46(1): 380-385.
  12. Khanipour S, Mehri M, Bagherzadeh‐Kasmani F, Maghsoudi A and Assadi Soumeh E (2019) Excess dietary tryptophan mitigates aflatoxicosis in growing quails. Journal of Animal Physiology and Animal Nutrition, 103(5): 1462-73.
  13. Kim K, Oh CM, Ohara-Imaizumi M, Park S, Namkung J, Yadav VK, Tamarina NA, Roe MW, Philipson LH, Karsenty G and Nagamatsu S (2015) Functional role of serotonin in insulin secretion in a diet-induced insulin-resistant state. Endocrinology, 156(2): 444-52.
  14. Liu Y, Yuan JM, Zhang LS, Zhang YR, Cai SM, Yu JH and Xia ZF (2015) Effects of tryptophan supplementation on growth performance, antioxidative activity, and meat quality of ducks under high stocking density. Poultry Science, 94(8): 1894-901.
  15. Mehri M (2014) Optimization of response surface and neural network models in conjugation with desirability function for estimation of nutritional needs of methionine, lysine, and threonine in broiler chickens. Poultry Science, 93(7): 1862-1867.
  16. Mir NA, Rafiq A, Kumar F, Singh V and Shukla V (2017) Determinants of broiler chicken meat quality and factors affecting them: a review. Journal of Food Science and Technology, 54(10): 2997-3009.
  17. Montgomery DC (2008) Design and Analysis of Experiments. John Wiley and Sons, Inc New York, USA. pp. 300-301.

 

 

 

  1. Myers RH and Montgomery DC (2009) Response surface methodology: process and product optimization under designed experiments. 3rd Wiley and Sons, New York. pp. 255-280.
  2. Patil RJ, Sirajudeen M, Tyagi JS, Moudgal RP and Mohan J (2013) In vivo Efficacy of Melatonin and L-Tryptophan Against Aflatoxin Induced Endogenous Toxicities in Broiler Chickens. Animal Nutrition and Feed Technology, 13(1): 117-124.
  3. Pierron A, Alassane-Kpembi I and Oswald IP (2016) Impact of mycotoxin on immune response and consequences for pig health. Animal Nutrition, 2(2): 63-68.
  4. Rosebrough RW (1996) Crude protein and supplemental dietary tryptophan effects on growth and tissue neurotransmitterlevels in the broiler chicken. British Journal of Nutrition, 76(1): 87-96.
  5. Salem R, El-Habashi N, Fadl SE, Sakr OA and Elbialy ZI (2018) Effect of probiotic supplement on aflatoxicosis and gene expression in the liver of broiler chicken. Environmental Toxicology and Pharmacology, 60: 118-127.
  6. Sirajudeen M, Kandasamy G, Jagbir ST, Ram Phal M, Jag M and Ram S (2009) Protective Effects of Melatonin in Reduction of Oxidative Damage and Immunosuppression Induced by Aflatoxin B1-Contaminated Diets in Young Chicks. Environmental Toxicology, 26(2): 153-160.
  7. Turkoz Y, Celik O, Hascalik S, Cigremis Y, Hascalik M, Mizrak B and Yologlu S (2004) Melatonin reduces torsion–detorsion injury in rat ovary: biochemical and histopathologic evaluation. Journal of Pineal Research, 37(2): 137-41.
  8. Zhang H, Yin J. Li D, Zhou X and Li X (2007) Tryptophan enhances ghrelin expression and secretion associated with increased food intake and weight gain in weanling pigs. Domestic Animal Endocrinology, 33: 47-61.