نوع مقاله : مقاله پژوهشی
نویسندگان
1 استاد، مؤسسۀ تحقیقات علوم دامی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.
2 دانشجوی دکتری، گروه سل و تحقیقات ریوی، انستیتو پاستور ایران، تهران، ایران.
3 دانشجوی دکتری، گروه میکروبشناسی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران.
چکیده
این آزمایش بهمنظور بررسی اثرات کربوهیدراتهای دیواره سلولی جیرههای غذایی مکملشده با آنزیم بر عملکرد و بیان ژنهای مؤثر در انتقال گلوکز (SGLT1 GLUT2)، پپتیدها (PepT1) و تولید موسین (MUC2) در روده باریک جوجه گوشتی انجام شد. در این مطالعه تعداد 1100 قطعه جوجه یکروزه مخلوط (جنس نر و ماده)، از سویه تجاری راس 308 در قالب طرح کاملاً تصادفی با 11 تیمار و پنج تکرار (20 پرنده در هر تکرار) بهمدت 42 روز استفاده شد. جیرههای آزمایشی بهترتیب شامل جیره شاهد، جیرههای حاوی گندم، جو، سبوس گندم، سبوس برنج و جو بدون پوشینه با و بدون آنزیم بودند. نتایج نشان داد که اثر جیرههای غذایی حاوی گندم، جو و جو بدون پوشینه با آنزیم بر وزن زنده کل جوجههای گوشتی در سن 42 روزگی دارای اختلاف معنیدار بود (p<0/05). کربوهیدراتهای دیواره سلولی گندم، سبوس گندم و سبوس برنج در جیرههای غذایی باعث افزایش فعالیت آنزیم آمیلاز لوزالمعده (جو 3/02، گندم 5/99 واحد به ازای میلی گرم پروتئین بافت روده باریک) شدند (p<0/05). بیان ژنهای SGLT1 و MUC2 مورد مطالعه در پرندگانی که جیرههای بدون آنزیم دریافت کردند بیشتر از پرندگانی بود که با جیره شاهد تغذیه شدند (p<0/05). بیان ژنهای مذکور تنها در پرندگانی که جیرههای حاوی سبوس گندم و سبوس برنج مکملشده با آنزیم دریافت کردند بیشتر از پرندگان شاهد بود (p<0/05). مکمل سازی جیرههای غذایی حاوی کربوهیدراتهای دیواره سلولی با آنزیم بر بیان ژنهای انتقال گلوکز (SGLT1 و GLUT2)، انتقال پپتید (PepT1) و تولید موسین (MUC2) در ژژنوم روده باریک تأثیرگذار است.
کلیدواژهها
عنوان مقاله [English]
The effect of feed cell wall carbohydrates on the expression of nutrient transport genes and mucin production in the small intestine of broilers
نویسندگان [English]
- Akbar Yaghobfar 1
- Rezvan Yaghoubfar 2
- Ehsan Zare Banadkoki 3
1 Aniaml Science Research Institiute
2 PhD study, Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran, rezvanyaghobfar@yahoo.com
3 PhD study, Department of microbiology, Shahed University of medical science, Tehran, Iran, ehsanzare89@gmail.com
چکیده [English]
The experiment was conducted to investigate the effects of cell wall carbohydrates with diet supplemented enzyme on the function and expression of glucose transporter genes (SGLT1 and GLUT2), peptide transporter (PepT1) and mucin production (MUC2) in the small intestine of broilers. In this study, 1100 mixeddayold chickens (male and female), Ross 308 were used based on a completely randomized design with 11 treatments and five replications (20 birds per replication) for 42 days. Experimental diets included control diets, diets containing wheat, barley, wheat bran, rice bran, and hull less barley with and without enzymes, respectively. The results showed that the effect of diets containing wheat, barley and hull less barley with enzyme on the total live weight of broiler chickens at 42 days of age was significantly different (P <0.05).Cell wall carbohydrates of wheat, wheat bran and rice bran in diets increased pancreatic amylase activity (barley 3.02, wheat 5.99 U/mg CP of small intestinal tissue) (P < 0.05). The expression of the studied SGLT1 and MUC2 genes in the experimental diets without enzyme showed a significant increase compared to enzymes supplemented diet (P < 0.05). Also, among the groups of enzyme-supplemented diets, only wheat and rice bran groups were able to increase the expression of SGLT1, MUC2 and GLUT2 genes compared to the control group (P <0.05). In conclusion, supplementation of diets containing cell wall carbohydrates with enzyme affects the expression of glucose transport genes (SGLT1 and GLUT2), peptide transport (PepT1) and mucin production (MUC2) in the small intestine jejunum. This indicates the optimal function of the digestive system of broilers in terms of digestion and absorption of nutrients.
کلیدواژهها [English]
- Broilers
- Cell wall carbohydrates
- Diets
- Enzyme activity
- Gene expression
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