نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکتری، گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی،دانشگاه علوم کشاورزی و منابع طبیعی خوزستان،ملاثانی، ایران.

2 استاد،گروه علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.

3 استادیار،گروه دامپزشکی دانشگاه آزاد اسلامی واحد یاسوج، یاسوج، ایران.

چکیده

تأثیر افزودنی‏های تنظیم کننده‏ pH شکمبه بر هیستومورفومتری و هیستوپاتولوژی بافت‌های شکمبه-نگاری و کبد با استفاده از بیست و چهار رأس بره نر عربی سه تا چهار ماهه با وزن 3/15± 23/9کیلوگرم در قالب طرح کاملا تصادفی با سه تیمار و هشت تکرار در یک دوره آزمایشی 77 روزه بررسی شد. تیمارهای آزمایشی شامل جیره‏ شاهد؛ جیره شاهد + بافر بی‏کربنات سدیم؛ جیره شاهد + باکتری مگاسفرا السدنی و مخمر ساکارومایسس سرویسیه (باکتری-مخمر) بودند. در پایان آزمایش بره‌ها کشتار شدند و از کبد و دستگاه گوارش آنها نمونه‌ گیری شد. ضخامت کل دیواره شکمبه-نگاری در بره‌های دریافت کننده‏ بافر بیشتر از گروه شاهد بود (0/05>P). ضخامت بافت پوششی شکمبه-نگاری در بره‌های دریافت کننده‏ بافر و باکتری–مخمر کمتر از گروه شاهد بود (0/05>P). ضخامت پرز شکمبه-نگاری در بره‌های شاهد از سایر تیمارها بیشتر بود (0/05>P). ضخامت لایه عضلانی نگاری در بره‌هایی که بافر دریافت کردند از سایر گروه‌ها بیشتر بود (0/05>P). هپاتیت پری پورتال بطور خفیف در کبد بره‌هایی که باکتری-مخمر دریافت کردند، دیده ‏شد.  بر اساس نتایج این آزمایش، مصرف عوامل تنظیم کننده‏ی pH، به‌ویژه باکتری-مخمر، در بره‌های تغذیه شده با مقادیر زیاد مواد متراکم از نظر هیستوپاتولوژی، آسیب‏های بافتی در شکمبه-نگاری و کبد را کاهش و ساختار بافتی شکمبه-نگاری را بهبود می‌بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of chemical buffer and Megasphaera elsdenii-yeast on histomorphometry and histopathology of rumen and liver of Arabian fattening lambs fed with concentrated diets

نویسندگان [English]

  • Omid Khorasani 1
  • Morteza Chaji 2
  • Farshad Baghban 3

1 Former PhD Student, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran

2 Faculty member (Academic staff) ِKhuzestan Agricultural Sciences and Natural Resources University

3 Department of Veterinary Medicine, Azad University of Yasoj, Yasij, Iran

چکیده [English]

The aim of this study was to investigate the effect of ruminal pH-adjusting additives on histomorphometry and histopathology of rumen - reticulum and liver tissues. Twenty-four Arabi male lambs with three to four months old and initial body weight of 23.9±3.15 kg were used in a completely randomized design with three treatments and eight replicates in a period of 77 days. The experimental treatments consisted of a control diet, control diet + sodium bicarbonate buffer (buffer), and control diet + Megasphaera elsdenii and Saccharomyces cerevisiae (bacterial- yeast). At the end of the experiment, lambs were slaughtered and samples were taken from the liver and gastrointestinal tract for tissue studies. Rumen-reticulum wall thickness in the buffer receiving lambs was greater than that of control group (p < 0.05). The thickness of the rumen-reticulum epithelium in the buffer and bacterial-yeast receiving lambs was less than the control group (p < 0.05). Rumen-reticulum papillae thickness was higher in control than other treatments (p < 0.05). The thickness of reticulum tunica muscularis in the buffer treatment was higher than other treatments (p < 0.05). Periportal hepatitis was seen mildly in the liver of bacterial- yeast receiving lambs. In according to the results of the present experiment, the use of pH regulators, especially bacterial-yeast, in lambs fed with high concentrate levels, in terms of histopathology, reduce tissue damages in the rumen- reticulum and liver and improve tissue structure of rumen-reticulum.

کلیدواژه‌ها [English]

  • Epithelium tissue
  • Muscularis tunica
  • Periportal hepatitis
  • Rumen papillae
  • Tissue damage

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