نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فارغ التحصیل از دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 استاد، عضو هیئت علمی گروه علوم دامی - دانشکده علوم دامی وشیلات - دانشگاه کشاورزی و منابع طبیعی ساری

3 دانشیار، عضو هیئت علمی گروه علوم دامی- دانشکده علوم دامی و شیلات- دانشکاه علوم کشاورزی و منابع طبیعی ساری

چکیده

هدف از پژوهش حاضر شناسایی تنوع های آللی ژن های C4-A و لاکتوفرین و بررسی ارتباط آنها با تعداد سلول های بدنی شیر در گاوهای هلشتاین بود. بیماری ورم پستان یکی از شایع ترین بیماری های گاوهای شیری است که تولید شیر را کاهش می دهد و هزینه های زیادی را به پرورش دهندگان تحمیل می کند. ژن های C4-A و لاکتوفرین از جمله ژن های تاثیرگذار در سیستم ایمنی برای مقابله با پاتوژن ها عوامل میکروبی و بیماری ورم پستان محسوب می شوند. شماره 384 نمونه خون از گاو هلشتاین تهیه و استخراج DNA با استفاده از روش نمکی بهینه یافته انجام شد. برای تعیین ژنوتیپ نمونه ها از فناوری آیپلکس (IPLEX) استفاده شد. برای شناسایی چندشکلی های تک نوکلئوتیدی جایگاه های نشانگر 1- rs137485678; C<G و2-rs132741478; C<T برای ژن کمپلمان C4-A و جایگاه های 1- ; A<Grs384176726 و2- rs445918028; A<T برای ژن لاکتوفرین انتخاب شدند. تعیین ژنوتیپ در جایگاه rs137485678; C<G از ژن C4-A دو آلل C و G با فراوانی های 0/58 و 0/42 و همچنین سه ژنوتیپ CC,CGو GG به ترتیب با فراوانی 0/31،0/53 و 0/16 را نشان داد. در سایر جایگاه ها چندشکلی مشاهده نشد. تجزیه و تحلیل نشانگر-صفت وجود ارتلباط معنی دار آماری (P<0/05) را نشان داد. به صورتی که گاوهای با ژنوتیپ CG کمترین تعداد سلول های بدنی را داشتند. برای شناسایی هاپلوتایپ از نرم افزار فاز (phase) استقفاده شد. براساس نتایج ای نتحقیق ناشنگر 1- rs137485678; C<G از ژ« C4-A می تواند به عنوان نشانگر ژنتیکی برای بهبود ورم پستان در گاوهای شیری مورد استفاده قرا گیرد.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of allelic variants of complement C4-A and Lactoferrin genes using iPLEX technique and its association with somatic cell count in Holstein cattle

نویسندگان [English]

  • Maryam Mohammadnezhad 1
  • Ghodrat Rahimi Mianji 2
  • Ayoub Farhadi 3

1 Graduated of master of Genetics and Animal Breeding of Sari Agricultural Sciences and Natural Resources University, Sari,

2 Professor, Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Sciences and Natural Resources University

3 Associate Professor, , Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Sciences and Natural Resources University, Sari

چکیده [English]

The aim of this study was to identify the allelic variants of C4-A and LF genes and to investigate their associations with the nunber of milk somatic cells in Holstein cows. Mastitis is one of the most common diseases in dairy cows, that reduces milk production and imposes high costs on breeders. C4-A and lactoferrin genes are amonge the genes that affect the immune system to fight pathogens, microbial agents and mastitis. 384 blood samples were prepared from Holstein cows and DNA extraction was performed using optimized salting out method. IPLEX technique was used to determine the genotype of the samples. To identify single nucleotide polymorphisms, the marker sites of 1-rs137485678; C<G and 2-rs132741478; C<T for C4- A complement gene and 1-rs384176726; A<G and 2-rs445918028; A<T were selected for the LF gene. Genotyping at 1-rs137485678; C<G locus of C4-A gene showed two C and G alleles with frequencies of 58 and 42 %, and also three genotypes CC, CG and GG with frequencies of 31, 53 and 16%, respectively. No polymorphisms were observed at the other sites. Marker-trait analysis showed a statistically significant association (P<0.05), so that cows with CG genotype had the lowest number of somatic cells. Phase software was used to identify haplotypes. Based on the results of this research, the marker rs137485678; C <G-1 of the C4-A gene can be used as a genetic marker to improve mastitis in dairy cows.

کلیدواژه‌ها [English]

  • Keywords: C4A Gene
  • iPLEX Technique
  • Lactoferrin Gene
  • Mastitis
  • somatic cells
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