نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، اهواز، ایران
2 دانشیار دانشکدۀ علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان
3 استاد،دانشگاه علوم کشاورزی و منابع طبیعی رامین، تخصص: ژنتیک و اصلاح نژاد دام و طیور
4 دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، تخصص: تغذیه نشخوارکنندگان/گاو شیری/ گوسفند/گاومیش/ روشهای آزمایشگاهی/ کشت پیوسته دو
5 دانشیار،پردیس ابوریحان، تخصص: ژنتیک و اصلاح نژاد دام/ ژنتیک مولکولی/ بیوانفورماتیک
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminal
tissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using the
Hiseq2500 illumine platform. Hisat2 software was used to align reads to the bovine reference genome and StringTie software package was used to
assemble read files into transcripts. Using next generation sequencing, 1636 genes belonging to known intergenic lncRNAs were identified, of which
56 genes showed significant differential expression (P≤0.05). Neighbor genes of known intergenic lncRNAs were determined on bovine genome.
Analysis of biological pathways and molecular function showed that five biological pathways were significantly (P≤0.05) enriched. These pathways
were Apelin signaling pathway, Gap junction, Glucagon signaling pathway, Renin secretion, and AGE-RAGE signaling pathway. Moreover, two
molecular functions including gap junction channel activity, and phosphatidyl inositol phospholipase C activity were significantly (P≤0.05) enriched.
Some lncRNAs have different expression in healthy and acidosis samples, and the decreased pH acts as a stimulus to activate some biological
signaling pathways. In conclusion, it was indicated that lncRNAs with differential expression between the control group and the group affected by
acidosis are associated with pathways related to rumen energy metabolism and signaling. Identified differentially expressed lncRNAs could be used as
prognostic in acidosis and biomarkers or promising candidates in animal breeding.
کلیدواژهها [English]