نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد،بخش علوم دام، دانشکده کشاورزی، دانشگاه شهید باهنر، کرمان، ایران.
2 دانشیار،هیات علمی بخش علوم دامی، دانشکده کشاورزی دانشگاه شهید باهنر کرمان
3 3. گروه بیوتکنولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران.
چکیده
هدف از این مطالعه، استفاده از باکتری‌های باسیلوسسابتیلیس با قابلیت تولید آنزیم فیتاز و تأثیر آن بر عملکرد رشد، اندامهای گوارشی و استحکام استخوان درشتنی در جوجه‌های گوشتی انجام گرفت. این آزمایش با 200 قطعه جوجهگوشتی یکروزه سویه راس 308 در قالب طرح کاملاً تصادفی با 5 تیمار، 4 تکرار و 10 قطعه جوجه در هر تکرار به‌مدت 42 روز اجرا شد. تیمارها شامل: تیمار1- جیره شاهدمثبت حاوی فسفر استاندارد; تیمار2- جیره شاهد منفی حاوی 30 درصد فسفر کم‌تر- تیمار3 ; جیره شاهد منفی + آنزیم فیتاز; تیمار4- جیره شاهد منفی + پروبیوتیک تجاری تیمار5- جیره شاهد منفی + باسیلوسسابتیلیس 3-17SH بودند. تغذیه جوجه‌هایگوشتی با جیره حاوی باکتری باسیلوسسابتیلیس 3-17SH، مصرف خوراک و میانگین افزایش وزن بدن را در کل دوره پرورش کاهش داد(0/05 >P)، تغذیه جوجه‌هایگوشتی با جیره حاوی آنزیم فیتاز، مصرف خوراک و میانگین افزایش وزن را افزایش داد (0/05>P). مقاومت استخوان درشتنی در پرندگانی که جیره حاوی آنزیم فیتاز را دریافت کردند بیش‌تر از سایر گروهها بود (0/05>P). براساس نتایج حاصل، استفاده از آنزیم فیتاز در جیرههای با کمبود فسفر، عملکرد را در جوجه‌هایگوشتی بهبود میدهد؛ اما باکتری باسیلوسسابتیلیس مولد فیتاز 3-17SH، نمی‌تواند جایگزین مناسبی برای پروبیوتیک و هم‌چنین آنزیم فیتاز باشد.
کلیدواژه‌ها
عنوان مقاله [English]
Effect of phytase producing Bacillus subtilis SH17-3, phytase enzyme and a commercial probiotic on the performance of broiler chickens fed low phosphorus diets
نویسندگان [English]
- MOSLEM ASADI KOROM 1
- Mohamad Salarmoini 2
- Mohsen Afsharmanesh 2
- Yaser Fattahian 3
1 Department of Animal Science, College of Agriculture, Shahid Bahonar University, Kerman, Iran.
2 Department of Animal Science, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
3 Department of Biotechnology, Environmental Sciences Research Institute, Advanced Science and Technology Institute of Environmental Sciences, University of Industrial and Technological Advanced Studies,Kerman,Iran.
چکیده [English]
The aim of this study was to evaluate the efficacy of Bacillus subtilis bacteria capable to produce phytase enzyme on improving broiler chicken performance and to evaluate its effect on gastrointestinal organs, and tibia strength in broiler chickens. This experiment was done using 200 one-day old broiler chicks (Ross 308), in a completely randomized design with 5 treatments, 4 replicates and 10 chicks per replicate for 42 days. The treatments include: 1) positive control diet containing sufficient phosphorus level; 2) negative control diet with 30% less phosphorus; 3) negative control diet supplemented with phytase enzyme; 4) negative control diet supplemented with a commercial probiotic; 5) negative control diet supplemented with Bacillus subtilis SH17-3. Feeding broiler chickens with a diet containing Bacillus subtilis bacteriaSH17-3 significantly reduced feed intake and mean body weight gain (BWG) in the total rearing period (P<0.05). Feeding broiler chickens with a diet containing phytase enzyme significantly increased feed intake and BWG (P<0.05). The strength of tibia was significantly increased in birds received phytase enzyme, compared to other groups (P<0.05). Based on the results the use of phytase enzyme in diets with phosphorus deficiency, improves performance in broilers; but Bacillus subtilis bacteria SH 17-3 could not be a good alternative for probiotic and also phytase enzyme.
کلیدواژه‌ها [English]
- "Bacillus subtilis"
- "Broiler chickens"
- "low phosphorus"
- "Phytate"
- "tibia"
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