نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری، گروه پرورش و مدیریت طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران.
2 استاد، گروه پرورش و مدیریت طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران.
3 استادیار، گروه پرورش و مدیریت طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران.
چکیده
هدف از این تحقیق بررسی اثر افزودن روغن‌های کتان(منبع امگا-3) و کنجد(منبع امگا-6) به همراه ویتامین E، برای بهبود فراسنجه‌های اسپرم، پروفایل اسیدچرب اسپرم، عملکرد باروی و جوجه‌درآوری خروس‌های مسن گله های مادرگوشتی بود. برای این منظور 24 قطعه خروس 45 هفته از سویه راس، به چهار تیمار با شش تکرار در طرح پایه کاملا تصادفی اختصاص داده شد. تیمارها شامل: الف- جیره پایه (شاهد)؛ ب- جیره حاوی دو درصد روغن کتان؛ ج- جیره حاوی دو درصد روغن کنجد؛ د- جیره حاوی یک درصد روغن کتان و یک درصد روغن کنجد بود. تمام تیمارها از نظر مقدار انرژی، پروتئین و ویتامین E یکسان بودند. خروس‌ها بهمدت 60 روز از جیره‌های آزمایشی تغذیه شدند و اسپرم‌گیریاز خروس‌ها در روزهای یک، 20، 40 و 60 آزمایش انجام شد. غلظت، جنبایی کل و پیش‌رونده و پراکسیداسیون غشای اسپرم خروس‌هایی که جیره حاوی دو درصد کتان و یا مخلوط مساوی از دو روغن را دریافت کردند با سایر تیمارها تفاوت معنی‌داری داشتند( 0/05>P). یکپارچگی غشا، زنده‌مانی، پروفایل اسیدچرب DHA اسپرم و میزان باروری در خروس‌هائی که با جیره حاوی دو درصد روغن کتان تغذیه شدند با سایر تیمارها تفاوت معنی داری داشت(0/05>P). براساس نتایج این تحقیق افزودن دو درصد روغن کتان و یا مخلوط یک درصد از هر یک از روغن های کتان و کنجد با حضور ویتامین E سبب بهبود عملکرد تولید مثلی خروس‌های مسن می‌شود.
کلیدواژه‌ها
عنوان مقاله [English]
The effects ofdietary Flaxseed and Sesame oils on reproductive performance of aged breeder broiler males
نویسندگان [English]
- hossein pasha zanussi 1
- farid shariatmadari 2
- Mohsen sharafi 3
- Hamed Ahmadi 3
1 head of educational system groups
2 university
3 department of poultry science /tarbiat modares university
چکیده [English]
This study was conducted to assess the effects of dietary flaxseed and sesame oils, on the semen parameters, fatty acid composition of sperm as well as the fertility and hatchability eggs from aged roosters. In a completely randomized design, 24 Ross-308 roosters (aged 45 week) assigned to four groups, comprising six replicates and one bird in each. The birds received different diets including basal diet (control), basal diet supplemented with 2% flaxseed oil (FO), basal diet supplemented with 2% sesame oil (SO) and basal diet supplemented with 1% flaxseed oil and 1% sesame oil (MO). The diets were iso-caloric and iso-nitrogenous, containing the same level of vitamin E. The roosters were fed diet for 60 days, during which semen samples were collected on 1st, 20th, 40th and 60th days and the samples were tested for different characteristics. The results indicated that different diets affected semen qualities, except semen volume and the morphology. The concentration, progressive motility, MDA as well as viability of sperms were significantly different during different times of the experiment. The sperm quality parameters including total and progressive motility as well as MDA turned out to improve in the roosters fed FO or MO. Furthermore, the integrity of sperm membrane, DHA and DPA concentration, as well as the fertility were higher in the treatment group containing FO. It seems that supplementation of aged rooster’s diet with flaxseed oil or mixed oils, together with vitamin E improves the semen qualities and it can be applied as an appropriate strategy to preserve the reproductive performance of aged rooters.
کلیدواژه‌ها [English]
- fertility
- hatchability: PUFA
- senescent rooster
- Sperm
2. Alizadeh A, Esmaeili V, Shahverdi A, Rashidi L (2014) Dietary fish oil can change sperm parameters and fatty acid profiles of ram sperm during oil consumption period and after removal of oil source. Cell Journal (16):289.
3. Avital-Cohen NR, Heiblum N, Argov-Argaman A, Rosenstrauch Y, Chaiseha N, Mobarkey and Rozenboim I (2013) Age-related changes in gonadal and serotonergic axes of broiler breeder roosters. Domestic Animal Endocrinology (44):145-150.
5. Bongalhardo DC, Leeson S, Buhr MM (2009) Dietary lipids differentially affect membranes from different areas of rooster sperm. Poultry Science (88): 1060-9.
6. Cerolini S, Kelso KA, Noble RC, Speake BK, Pizzi F and Cavalchini LG (1997) Relationship between spermatozoan lipid composition and fertility during aging of chickens. Biology Reproduction (57): 976-80.
7. Cerolini S, Gliozzi TM, Pizzi F, Parodi L, Maldijian A and Noble R (2002) Relationship between lipid components and cell functions in spermatozoa of domestic animals. Program Nutrition 4(2): 1-4.
8. Cerolini S,Pizzi F, Gliozzi T, Maldjian A, Zaniboni L and Parodi L (2003) Lipid manipulation of chicken semen by dietary means and its relation to fertility: a review. Worlds Poultry Science Journal (59): 65-75.
9. Cerolini S, Surai PF, Speake BK, Sparks NHC (2005) Dietary fish and evening primrose oil with vitamin E effects on semen variables in cockerels. British Poultry Science (46):214–22.
10. Cerolini S, Zaniboni L, Maldjian A, Gliozzi T(2006) Effect of docosahexaenoic acid and α- tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology (66): 877-86.
11. Chen W, Jiang YY, Wang JP, Huang YQ, Wang ZX (2014) Effects of dietary flaxseed meal on production performance, egg quality, and hatchability of Huoyan geese and fatty acids profile in egg yolk and thigh meat from their offspring. Livestock Science (164): 102-8.
12. Esmaeili V, Shahverdi AH, Moghadasian MH and Alizadeh AR (2015) Dietary fatty acids affect semen quality: a review. Andrology 3: 450-461.
13. Feng Y, Ding Y, Liu Y, Tian Y, Yang S, Guan andZhang C (2015) Effects of dietary omega- 3/omega-6 fatty acid ratio's on reproduction in the young breeder rooster. BMC Veterinary Research (11): 73.
14. Kanu PJ, Bahsoon JZ, Kanu JB and Kandeh JBA (2010) Nutraceutical importance of sesame seed and oil: a review of the contribution of their lignans. Sierra Leone Journal of Biomedical Research (2): 4-16.
15. Kelso KA, Cerolini S, Noble RC, Sparks NHC, Speake BK (1996) Lipid and antioxidant changes in semen of broiler fowl from 25 to 60 weeks of age. Journal of Reproduction and Fertility (106): 201-206.
16. Kelso KA, Cerolini S, Noble RC, Sparks NHC, Speake BK (1997) The effects of dietary supplementation with docosahexaenoic acid on the phospholipid fatty acid composition of avian spermatozoa. Comp. Biochemistry Physiology Part B Biochemistry Molecular Biology (118): 65-69.
17. Kelso KA, Cerolini S, Speake BK, Cavalchini LG, Noble RC (1997) Effects of dietary supplementation with α-linolenic acid on the phospholipid fatty acid composition and quality of spermatozoa in cockerel from 24 to 72 weeks of age. Journal of Reproduction Fertility (110): 53-59.
18. Moallem U, Neta N, Zeron Y, Zachut M and Roth Z (2015) Dietary α-linolenic acid from flaxseed oil or eicosapentaenoic and docosahexaenoic acids from fish oil differentially alter fatty acid composition and characteristics of fresh and frozen-thawed bull semen. Theriogenology (83): 1110-20.
19. Muhammad Zand Mubashir Ali K (2016) Review on effects of dietary polyunsaturated fatty acids on semen quality of birds and mammals. Asian Journal of Agriculture Biology 4(4): 134-139.
20. Ommati MM, Zamiri MJ, Akhlaghi A, Atashi H, Jafarzadeh MR and Rezvani MR(2013) Seminal characteristics, sperm fatty acids, and blood biochemical attributes in breeder roosters orally administered with sage (Salvia officinalis) extract. Animal Production Scince (53): 548-54.
21. Safari Asl R, Shariatmadari F, Sharafi M, Karimi Torshizi MA and Shahverdi A (2018) Improvements in semen quality, sperm fatty acids, and reproductive performance in aged Ross breeder roosters fed a diet supplemented with a moderate ratio of n-3: n-6 fatty acids. Poultry Science (0): 1-9.
22. Shahverdi A, Sharafi M, Gourabi H, Yekta AA, Esmaeili V and Sharbatoghli M (2015) Fertility and flowcytometric evaluations of frozen-thawed rooster semen in cryopreservation medium containing low-density lipoprotein. Theriogenology 83(1): 78-85.
23. Surai, PF, Fujihara N, Speake BK, Brillard JP, Wishart GJ and Sparks NHC (2001) Polyunsaturated fatty acids, lipid peroxidation and antioxidant protection in avian semem. Asian Australas. Journal Animal Science (14): 1024-1050.
24. Zanini SF, Torres CAA, Bragagnolo N, Turatti JM, Silva MG and Zanini MS (2003) Evaluation of the ratio of ω6: ω3 fatty acids and vitamin E levels in the diet on the reproductive performance of cockerels. Arch Animal Nutrition (57): 429-42.
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