اثر استفاده از مکمل کروم - متیونین بر کاهش اثرات منفی ناشی از تنش فیزیولوژیک در جوجه های گوشتی

نوع مقاله : مقاله پژوهشی

نویسنده

استادیار پژوهشی، سازمان پژوهش های علمی و صنعتی ایران، پژوهشکده کشاورزی، تخصص: تغذیه گوساله و گاو شیری/ ارزشیابی مواد خوراکی و افزودنی‌های خوراکی دام/ پروبیوتیک

چکیده

هدف این مطالعه بررسی اثر مکمل کروم - متیونین بر فراسنجه‌های خونی و ایمنی جوجه‌های گوشتی تحت تنش حاد بود. از 126 قطعه جوجه نر نژاد راس در قالب طرح کاملاً تصادفی با سه تیمار و سه تکرار و 14 قطعه پرنده در هر تکرار استفاده شد. تیمارها عبارت بودند از 1) جیره پایه بدون مکمل کروم و تنش (شاهد منفی)، 2) جیره پایه بدون مکمل کروم و تحت تنش (شاهد مثبت)، 3) جیره پایه با 1000 ppb مکمل کروم و تحت تنش. با افزودن 5/1 میلی‌گرم دگزامتازون در کیلوگرم جیره از روز 18 به مدت یک هفته تنش ایجاد شد و بعد از آن آزمایش تا 46 روزگی ادامه یافت. خونگیری در سن 25 و 46 روزگی انجام شد. تنش سبب افزایش غلظت گلوکز و چربی های سرم خون شد. در 25 روزگی، تغذیه جیره حاوی کروم در پرندگان تحت تنش، سبب کاهش مقدار تری گلیسرید، کلسترول، LDL و VLDL خون شد (05/0 > (P. در 46 روزگی، پرندگان تحت تنشی که کروم را در جیره خود مصرف کردند از تری گلیسرید، LDL ،VLDL بیشتر و HDL کمتری نسبت به دو گروه دیگر برخــوردار بود (05/0 > P). در پرندگان تحت تنش تعداد گلبول‌های سفید، درصد هتروفیل و نسبت هتروفیل به لنفوسیت افزایش یافت (05/0P < ) و در پرندگانی که مکمل کروم مصرف کردند این صفات کاهش یافت. نتایج این تحقیق نشان داد استفاده از مکمل کروم در شرایط تنش حاد می‌تواند نشانه‌های فیزیولوژیک بروز تنش را کاهش دهد اما ادامه مصرف آن بعد از رفع عامل تنش زا، اثر مثبتی بر این فراسنجه‌ها ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

Impact of chromium-methionine supplementation on decreasing deleterious effects of physiological stress in broilers

نویسنده [English]

  • maryam bagheri Varzaneh
Assistant Professor
چکیده [English]

This study was conducted to investigate the effects of chromium-methionine (Cr-Met) supplementation
on the blood and immune parameters of broiler under acute stress. A total of one hundred twenty six broilers were allocated to 3 treatments and 3 replicates (14 birds per replicate) in a completely randomized design. Treatments were: 1) negative control: i.e. basal diet without stress and Cr-Met, 2) positive control: basal diet with stress and without Cr-Met, 3) basal diet with stress and supplemented with 1000 ppb Cr-Met. Stress was induced from day 18 by addition of 1.5 mg dexamethasone per kg of the diet for one week followed by a withdrawal period until day 46. Blood samples were collected on day 25 and 46 of age. Results showed that stress increased blood glucose and lipid concentrations of serum (P < 0.05). The addition of Cr-Met decreased cholesterol, TG, LDL and VLDL on day 25 of age (P < 0.05). However, broilers fed Cr-Met had higher TG, LDL, VLDL and less HDL compared to other treatments on day 46 (P < 0.05). Administration of dexamethasone significantly elevated concentrations of white blood cell (WBC), heterophil (H), and heterophil to lymphocyte ratio (P < 0.05), while these parameters decreased by Cr-Met supplementation on day 25 (P < 0.05). In conclusion, supplemental Cr-Met improved broiler response to physiological stress during the acute stress period. However, broilers did not benefit from feeding Cr-Met supplementation after dexamethasone induced stress.

کلیدواژه‌ها [English]

  • broiler
  • Dexamethasone
  • Immunity
  • Organic chromium
  • Stress
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