تأثیر پری‌بیوتیک مانان ‌الیگوساکارید بر عملکرد، جمعیت میکروبی ایلئوم روده، صفات ایمنی و قابلیت هضم ایلئومی مواد مغذی در مرغ‌های تخم‌گذار تغذیه شده با جیره‌های حاوی سطوح مختلف پروتئین خام

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار گروه علوم دام و طیور - پردیس ابوریحان - تخصص:‌تغذیه طیور

2 دانشجوی فارغ التحصیل

3 استادیار دانشگاه صنعتی اصفهان دانشکده کشاورزی گروه علوم دامی

4 کارشناس آزمایشگاه ژنتیک دام

چکیده

این مطالعه به منظور بررسی تأثیر پری­بیوتیک مانان­الیگوساکارید برعملکرد، کیفیت تخم­مرغ، پاسخ ایمنی،جمعیتمیکروبی ایلئوم رودهوقابلیت هضمایلئومیموادمغذیدرمرغ­های تخم­گذار تغذیه شده با سطوح مختلف پروتئین خام انجام گرفت. از تعداد 150 قطعه مرغ تخم­گذار سویه های ­لاین W-36در قالب یک طرح کاملاً تصادفی با آزمایش فاکتوریل 2×3 استفاده شد. جیره­های آزمایشی شامل سه سطح پروتئین خام (سطح توصیه شده سویه، 95 و 90 درصد سطح توصیه شده) و دو سطح مانان­الیگوساکارید (صفر و 1/0 درصد جیره) بودند. کاهش 10 درصدی سطح پروتئین خام جیره سبب کاهش وزن تخم­مرغ، درصد تخم­گذاری، توده تخم­مرغ و نامناسب شدن ضریب تبدیل خوراک شد (01/0P<). افزودن مکمل مانان­الیگوساکارید به جیره، درصد تخم­گذاری و توده تخم­مرغ را بهبود داد (05/0P<).غلظت پادتن علیه نیوکاسل، پاسخ اولیه و ثانویه غلظت پادتن علیه گلبول قرمز گوسفندی در اثر مصرف مانان­الیگوساکارید در جیره افزایش یافتند (05/0P<). کاهش پنج درصدی سطح پروتئین خام جیره سبب کاهش مقدار تری­گلیسرید سرم خون شد (05/0P<). کاهش پنج و 10 درصدی پروتئین خام قابلیت هضم خاکستر کل را بهبود و کاهش 10 درصدی پروتئین خام قابلیت هضم چربی خام را کاهش داد (05/0P<). افزودن مانان­الیگوساکارید به جیره، قابلیت هضم چربی خام را بهبود و جمعیت میکروبی اشریشیاکلی را در ایلئوم روده کاهش داد (05/0P<).بنابراین، افزودن مکمل مانان­الیگوساکارید به میزان 1/0 درصد جیره موجب بهبود فاکتورهای ایمنی و فلور میکروبی ایلئوم روده شد. همچنین، سطح 68/15 درصد پروتئین خام جیره از سن 47 تا 57 هفتگی فراسنجه­های عملکردی مرغ­های تخم­گذار را کاهش نداد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of mannan-oligosaccharide prebiotic on performance, intestine ileum microflora, immune traits and ileum nutrient digestibility in laying hens fed diets with different crude protein levels

نویسنده [English]

  • shokoufe ghazanfari 1
چکیده [English]

This study was carried out toinvestigate the effect ofmannan-oligosaccharide prebioticonperformance,egg quality, immune response, intestine ileum microflora and nutrient digestibility in laying hens fed various levels of crude protein. A total of 150 Hy-Line W-36 layinghens were assigned to a 2×3 factorial arrangement of treatments. Experimental diets consisted of 3 levels of crude protein (recommended level for strain, 90 and 95% of recommended level) and 2 levels of mannan-oligosaccharides (0 and 0.1% of diet). Reduction of crude protein to 10% of basal diet resulted in decrease of egg weight, egg production, egg mass and feed conversion ratio (P<0.01). Supplementation of mannan-oligosaccharide into the diet improved egg production and egg mass (P<0.05). Newcastle antibody titer, primary and second antibody response against sheep red blood cell increased by supplementation of mannan-oligosaccharides (P<0.05). Reduction of crude protein to 5% reduced blood serum triglycerides concentration (P<0.05). Reduction of crude protein to 5 and 10 % of diet caused increases in digestibility coefficients of organic matter and Reduction of crude protein to 10 % caused decline of crude fat digestibility (P<0.05). Dietary supplementation mannan-oligosaccharides increased crude fat digestibility and decreased ileal E. coli count (P<0.05). So, supplementation of mannan-oligosaccharide at levels of 0.1% of diet improved immune parameters and intestinal ileum microflora. Similarly, crude protein at levels of 15.68% of diet did not reduced performance parameters of layinghens from age 47 to 57 weeks.

کلیدواژه‌ها [English]

  • crude protein
  • laying hens
  • nutrient digestibility
  • Prebiotic
  • production performance
1.      Alzueta C, Rodríguez ML, Ortiz LT, Rebolé A and Treviño J (2010) Effects of inulin on growth performance, nutrient digestibility and metabolisable energy in broiler chickens. British Poultry Science. 51(3):393-398.
2.      Angelovicova M (1994) Economic use of a low-protein feed mixture in layer diet. Zivocisna Vyroba-UZPI (Czech Republic). 39: 1048-1062.
3.      Berry WD and Lui P (2000) Egg production, egg shell quality and bone parameters in broiler breeder hens receiving Bio-Mos and Eggshell ]9. Poultry Science. 79: 124 (Abstract).
4.      Bedford MR (1995) Mechanism of action and potential environmental benefits from the use of feed enzymes. Animal Feed Science and Technology. 53(2): 145-155.
5.      Biggs P, Parsons CM and Fahey GC (2007) The effects of several oligosaccharides on growth performance, nutrient digestibilities, and cecal microbial populations in young chicks. Poultry Science. 86(11): 2327-2336.
6.      Bunchasak C, Poosuwan K, Nukraew R, Markvichitr K and Choothesa A (2005) Effect of dietary protein on egg production and immunity responses of laying hens during peak production period. International Journal of Poultry Science. 4(9): 701-708.
7.      Chen TT, Tsay SM, Yu CY, Weng BC and Chen KL (2006) Effects of dietary β-glucan supplementation against Eimeria tenella infection and immune parameter in male Leghorn chicks. Journal of the Chinese Society of Animal Science. 35: 101-108.
8.      Doeschate RAHM and Kenyon S (1999) Alternatives to antibiotic growth promoters: mannanoligosaccharides and organic acids. In: L.G. Cavalchini, D. Baroli (Editors). Proceedings of XIV European Symposium on the Quality of Poultry Meat. Bologna (Italy). Worldʼs Poultry Science Journal. pp. 203-207
9.      El-Samee LD, El-Wardany I, Ali NG and Abo-El-Azab OM (2012) Egg quality, fertility and hatchability of laying quails fed diets supplemented with organic zinc, chromium yeast or mannan oligosaccharides. International Journal of Poultry Science. 11(3): 221-224.
10.   Finuance MC, Dawson KA, Spring P and Newman KE (1999) The effect of mannan oligosaccharide on the composition of the microflora in turkey poults. Poultry Science. 78(1): 77-80.
11.   Ghiyasi M, Rezaei M, Sayyahzadeh H, Firouzbakhsh F and Attar A (2010) Effects of prebiotic (Fermacto) in low protein diet on some blood parameters and intestinal microbiota of broiler chicks. Italian Journal of Animal Science. 7(3): 313-320.
12.   Haghighi HR, Gong J, Gyles CL, Hayes MA, Sanei B, Parvizi P, Gisavi H, Chambers JR and Sharif S (2005) Modulation of antibody-mediated immune response by brobiotics in chickens. Clinical and Diagnostic Laboratory Immunology. 12: 1387-1392.
13.   Hassan HA and Ragab MS (2007) Single and combined effects of mannan oligosaccharide (mos) and dietary protein on the performance and immunity response of laying hens. Egyptian Poultry Science. 27: 969-987.
14.   Hy-Line International (2007). Hy-Line W-36 Commercial Management Guide. Hy-Line International, West Des Moines, IA, USA.
15.   Kashani S, Mohebbifar A, Habibian M and Torki M (2014) Effects of phytase supplementation of low protein diets on performance, egg quality traits and blood biochemical parameters of laying hens. Annual Research and Review in Biology. 4(4): 684-698.
16.   Keshavarz K and Austic RE (2004) The use of low-protein, low-phosphorus, amino acid-and phytase-supplemented diets on laying hen performance and nitrogen and phosphorus excretion. Poultry Science. 83(1): 75-83.
17.   McCarthy JF, Aherene FX and Okai DB (1974) Use of HCl insoluble ash as an index material for determining apparent digestibility with pigs. Canadian Journal of Animal Science. 54: 107–109.
18.   Meng QW, Yan L, Ao X, Jang HD, Cho JH and Kim IH (2010) Effects of chito-oligosaccharide supplementation on egg production, nutrient digestibility, egg quality and blood profiles in laying hens. Asian-Australasian Journal of Animal Sciences. 23(11): 1476-1481.
19.   Mızrak C, Yenice E, Kahraman Z, Tunca M, Yıldırım U and Ceylan N (2014) Effects of dietary sepiolite and mannanoligosaccharide supplementation on the performance, egg quality, blood and digestion characteristics of laying hens receiving aflatoxin in their feed. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 61: 65-71.
20.   Radu-Rusu CG, Pop IM and Simeanu D (2010) Effect of a synbiotic feed additive supplementation on laying hens performance and eggs quality. Lucrări Ştiinţifice, Seria Zootehnie. 53: 89-93.
21.   Sadeghi AA, Mohammadi A, Shawrang P and Aminafshar M (2013) Immune responses to dietary inclusion of prebiotic-based mannan-oligosaccharide and β-glucan in broiler chicks challenged with salmonella enteritidis. Turkish Journal of Veterinary and Animal Science. 37(2): 206-213.
22.   SAS )2005) SAS User′s guideStatistics. Version 8. SAS Inst. Inc., Cary, NC, USA.
23.   Shashidhara RG and Devegowda G (2003) Effect of dietary mannan oligosaccharide on broiler breeder production traits and immunity. Poultry Science. 82(8): 1319-1325.
24.   Spring P, Wenk C, Dawson KA and Newman KE (2000) The effects of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks. Poultry Science. 79(2): 205-11.
25.   Toghyani M, Gheiasari A, Ghalamkari G and Eghbalsaied SH (2011) Evaluation of cinnamon and garlic as antibiotic growth promoter substitutions on performance, immune responses, serum biochemical and haematological parameters in broiler chicks. Livestock Science. 138: 167-173.
26.   Wasilewska E, Ratowska J, Bielecka M, Zduńczyk Z and Jankowski J (2010) Mannanoligosaccharides or flavomycin in turkeys diets: effect on mucosa-associated microflora and growth performance. Journal of Animal and Feed Sciences. 19: 559-612
27.   Zaghini A, Martelli G, Roncada P, Simioli M and Rizzi L (2005) Mannanoligosaccharides and aflatoxin B1 in feed for laying hens: effects on egg quality, aflatoxins B1 and M1 residues in eggs, and aflatoxin B1 levels in liver. Poultry Science. 84(6): 825-32.
28.   Zdunczyk Z, Juskiewicz J, Jankowski J, Biedrzycka E and Koncicki A (2005) Metabolic response of the gastrointestinal tract of turkeys to diets with different levels of mannan-oligosaccharide. Poultry Science. 84(6): 903-909.