نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار گروه علوم دام و طیور - پردیس ابوریحان - تخصص:‌تغذیه طیور

2 دانشجوی فارغ التحصیل

3 استادیار دانشگاه صنعتی اصفهان دانشکده کشاورزی گروه علوم دامی

4 کارشناس آزمایشگاه ژنتیک دام

چکیده

این مطالعه به منظور بررسی تأثیر پری­بیوتیک مانان­الیگوساکارید برعملکرد، کیفیت تخم­مرغ، پاسخ ایمنی،جمعیتمیکروبی ایلئوم رودهوقابلیت هضمایلئومیموادمغذیدرمرغ­های تخم­گذار تغذیه شده با سطوح مختلف پروتئین خام انجام گرفت. از تعداد 150 قطعه مرغ تخم­گذار سویه های ­لاین W-36در قالب یک طرح کاملاً تصادفی با آزمایش فاکتوریل 2×3 استفاده شد. جیره­های آزمایشی شامل سه سطح پروتئین خام (سطح توصیه شده سویه، 95 و 90 درصد سطح توصیه شده) و دو سطح مانان­الیگوساکارید (صفر و 1/0 درصد جیره) بودند. کاهش 10 درصدی سطح پروتئین خام جیره سبب کاهش وزن تخم­مرغ، درصد تخم­گذاری، توده تخم­مرغ و نامناسب شدن ضریب تبدیل خوراک شد (01/0P<). افزودن مکمل مانان­الیگوساکارید به جیره، درصد تخم­گذاری و توده تخم­مرغ را بهبود داد (05/0P<).غلظت پادتن علیه نیوکاسل، پاسخ اولیه و ثانویه غلظت پادتن علیه گلبول قرمز گوسفندی در اثر مصرف مانان­الیگوساکارید در جیره افزایش یافتند (05/0P<). کاهش پنج درصدی سطح پروتئین خام جیره سبب کاهش مقدار تری­گلیسرید سرم خون شد (05/0P<). کاهش پنج و 10 درصدی پروتئین خام قابلیت هضم خاکستر کل را بهبود و کاهش 10 درصدی پروتئین خام قابلیت هضم چربی خام را کاهش داد (05/0P<). افزودن مانان­الیگوساکارید به جیره، قابلیت هضم چربی خام را بهبود و جمعیت میکروبی اشریشیاکلی را در ایلئوم روده کاهش داد (05/0P<).بنابراین، افزودن مکمل مانان­الیگوساکارید به میزان 1/0 درصد جیره موجب بهبود فاکتورهای ایمنی و فلور میکروبی ایلئوم روده شد. همچنین، سطح 68/15 درصد پروتئین خام جیره از سن 47 تا 57 هفتگی فراسنجه­های عملکردی مرغ­های تخم­گذار را کاهش نداد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of mannan-oligosaccharide prebiotic on performance, intestine ileum microflora, immune traits and ileum nutrient digestibility in laying hens fed diets with different crude protein levels

نویسنده [English]

  • shokoufe ghazanfari 1

1

2

3

4

چکیده [English]

This study was carried out toinvestigate the effect ofmannan-oligosaccharide prebioticonperformance,egg quality, immune response, intestine ileum microflora and nutrient digestibility in laying hens fed various levels of crude protein. A total of 150 Hy-Line W-36 layinghens were assigned to a 2×3 factorial arrangement of treatments. Experimental diets consisted of 3 levels of crude protein (recommended level for strain, 90 and 95% of recommended level) and 2 levels of mannan-oligosaccharides (0 and 0.1% of diet). Reduction of crude protein to 10% of basal diet resulted in decrease of egg weight, egg production, egg mass and feed conversion ratio (P<0.01). Supplementation of mannan-oligosaccharide into the diet improved egg production and egg mass (P<0.05). Newcastle antibody titer, primary and second antibody response against sheep red blood cell increased by supplementation of mannan-oligosaccharides (P<0.05). Reduction of crude protein to 5% reduced blood serum triglycerides concentration (P<0.05). Reduction of crude protein to 5 and 10 % of diet caused increases in digestibility coefficients of organic matter and Reduction of crude protein to 10 % caused decline of crude fat digestibility (P<0.05). Dietary supplementation mannan-oligosaccharides increased crude fat digestibility and decreased ileal E. coli count (P<0.05). So, supplementation of mannan-oligosaccharide at levels of 0.1% of diet improved immune parameters and intestinal ileum microflora. Similarly, crude protein at levels of 15.68% of diet did not reduced performance parameters of layinghens from age 47 to 57 weeks.

کلیدواژه‌ها [English]

  • crude protein
  • laying hens
  • nutrient digestibility
  • Prebiotic
  • production performance
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