تأثیر چند‌شکلی ژن BMP15 بر صفات رشد و تولید‌مثل گوسفند لری‌ بختیاری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه آزاد اسلامی واحد شهرکرد

2 دانشگاه آزاد اسلامی- واحد شهرکرد

چکیده

این پژوهش با هدف بررسی چند‌شکلی ناحیه‌ی اینترون ژنBMP-15 و تاثیر آن بر صفات رشد و تولید‌مثل در گوسفند نژاد لری‌بختیاری با استفاده از روش توالی‌یابی انجام شد. برای این منظور تعداد 100 نمونه خون (80 راس میش و 20راس قوچ) از ایستگاه‌‌ پرورش و اصلاح نژاد گوسفند لری‌بختیاری شهرکرد جمع‌آوری وDNA آنها استخراج شد. یک قطعه bp  356 از ژن BMP-15 با استفاده از واکنش زنجیره‌ای پلیمراز (PCR) توسط یک جفت آغازگر اختصاصی تکثیر و کیفیت آن توسط الکتروفورز بر روی ژل آگارز یک درصد بررسی شد. سپس توالی محصول PCR تعیین شد. جهت شناسایی چندشکلی‌ها، توالی‌های حاصله با توالی ثبت شده در بانک جهانی ژن مقایسه گردید. نتایج تنها حاکی از وجود یک جهش حذفیD75A) ) در این ژن بود که در این جهش آدنین با فراوانی 2/41 درصد در نوکلئوتید شماره 75 حذف شده بود. تأثیر این جهش در هیچ کدام از صفات رشد و تولیدمثل معنی‌دار نبود. مطالعه چندشکلی ژنتیکی در سایر بخش‌های این ژن به منظور بررسی بهتر ارتباط آن با صفات رشد و تولیدمثل توصیه می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of BMP15 gene polymorphism on growth and reproduction traits in Lori-Bakhtiari sheep

نویسندگان [English]

  • Parisa Mahvari 1
  • Abbas Doosti 1
1 Islamic Azad University Shahrekord Branch
2 Islamic Azad University Shahrekord Branch
چکیده [English]

The aim of this research was to recognize genetic polymorphism in intron place of BMP15 gene and its relationship with growth and reproduction traits in Lori-Bakhtiari sheep breed using sequencing method. Blood samples of 80 ewes and 20 rams of Lori-Bakhtiari sheep from breeding station of Shahrekord were collected. DNA was extracted from whole blood and their quality was evaluated using 1% agarose gel electrophoresis. A fragment with the size of 356 bp from extracted DNA was amplified using polymerase chain reaction (PCR) with a pair of specific primers. The PCR products were sequenced. Sequenced result was compared with sequenced gene in world gene bank by Blast program in NCBI. Results founds only a deletion mutation (D75A) in this gene that in this mutation thymine with 41.2% frequency was deleted .This mutation wasn't effect on reproduction and growth traits. Of course, it's better than polymorphisms in other segments of this gene were studied and then decided about effect of this gene on reproduction and growth trait.

کلیدواژه‌ها [English]

  • Bone morphogenetic proteins (BMP15)
  • Genetic Polymorphism
  • growth traits
  • Lori-Bakhtiari sheep breed
  • Reproductive traits
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