اثر سطوح مختلف مکمل نوکلئوتید بر عملکرد، سامانه ایمنی، ریخت شناسی روده کوچک و جمعیت میکروبی ایلئوم جوجه‌های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترا

2 استاد دانشگاه فردوسی مشهد، تخصص: تغذیه طیور

3 عضو هیأت علمی

چکیده

اثر مصرف سطوح مختلف مکمل نوکلئوتید در جیره بر عملکرد، خصوصیات لاشه، وضعیت سامانه ایمنی، ریخت شناسی روده کوچک و جمعیت میکروبی ایلئوم با استفاده از 308 قطعه جوجه گوشتی یک روزه سویه تجاری راس 308 مخلوط هر دو جنس در قالب طرح کاملاً تصادفی با چهار تیمار، هفت تکرار و 11 قطعه جوجه در هرتکرار به مدت 42 روز بررسی شد. تیمارهای آزمایشی شامل جیره پایه بر اساس ذرت و کنجاله سویا(شاهد) و جیره پایه حاوی سطوح 05/0، 1/0 و 2/0 درصد مکمل نوکلئوتید بودند. مصرف خوراک، افزایش وزن بدن و ضریب تبدیل غذایی تحت تأثیر تیمارهای آزمایشی قرار نگرفتند. نتایج پاسخ سامانه ایمنی هومورالدر سن 35 روزگی نشان داد که غلظت IgMسرم خون در تیمار 2/0 درصد نوکلئوتید نسبت به تیمار 05/0 درصد بیشتر بود (05/0P<).غلظت IgGسرم خون در سن 35 روزگی در تیمار 05/0 درصد نوکلئوتید بیشتر از تیمار 2/0 درصد بود (05/0P<). در سن 42 روزگی، غلظت IgG در تیمار 1/0 درصد مکمل نوکلئوتید نسبت به تیمار شاهد کمتر بود (05/0P<).پاسخ ایمنی سلولی در تیمار حاوی 1/0 درصد مکمل نوکلئوتید در 48 ساعت پس از تزریق فیتوهماگلوتینین بالاتر از تیمار شاهد بود(05/0P<). براساس نتا‌یج ا‌ین آزما‌یش، استفاده از مکمل نوکلئوتیدی پاسخ ایمنی سلولی جوجه‌های گوشتی رابهبودمی­بخشد اما تاثیری بر سایر فراسنجه‌ها ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of different dietary supplementation levels of nucleotide on performance, immune system, small intestine morphology and ileal microbial population of broiler chickens

نویسنده [English]

  • Ahmad Hassanabadi 2
چکیده [English]

Effect of different dietary levels of nucleotide supplementation on performance, carcass characteristics, immune system status, small intestine morphology and ileal microbial population of broiler chickens was evaluated using 308 one-day-old mixed-sex Ross 308 broilers in a completely randomized design with 4 treatments and 7 replicates of 11 chicks in each, up to 42 days of age. Experimental treatments included a corn-soybean meal basal diet (control), and the basal diet containing 0.05, 0.1 and 0.2 percent nucleotide supplementation. The results showed that feed intake, body weight gain and feed conversion ratio of the broilers were not significantly affected by experimental treatments. Results of humeral immune system response at 35 days of age showed that the blood serum IgM concentration in 0.2 percent nucleotide group was higher than 0.05 percent group(P< 0.05). Also, Serum IgG concentration in this age in 0.05 percent nucleotide group was higher than 0.2 percent group(P< 0.05). At 42 days of age, serum IgG concentration in 0.1 percent nucleotide treatment was lower than control group (P< 0.05). The cellular immune system response of chicks fed diet supplemented with 0.1 percent nucleotide at 48 hours after Phytohemagglutinin-P injection was higher than control treatment (P<0.05). On the basis of current experiment results, supplementation of broiler dietswith nucleotide improves cellular immune system response but has no significant effect on other parameters of broiler chickens.

کلیدواژه‌ها [English]

  • Carcass yield
  • Immunoglobulin
  • Intestinal villi
  • Lymphatic organs
  • weight gain
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