نوع مقاله : مقاله پژوهشی

نویسنده

عضو هیات علمی

چکیده

هدف از این پژوهش مقایسه CNV در تعدادی از نژاد­های گوسفند ایتالیا با گاو هلشتاین بود. از 580 راس گاو نر هلشتاین و 360 راس گوسفند نمونه خون تهیه و  DNAآن استخراج شد. ژنوتیپ چند شکلی­های تک نوکلئوتیدی سراسر ژنوم با تراشه 50k گاوی و گوسفندی در هر دو گونه تعیین شد. با استفاده از نرم افزار PennCNV تعداد 904 تنوع با میانگین طول kb 7/154 و میانه kb 7/103 در گوسفند و 744 مورد با میانگین طول kb 7/213 و میانه kb 5/124 در گاو شناسایی شد. کل طول این تنوع در ژنوم گوسفند Mb 5/8 و در ژنوم گاو Mb 80 بود. پس از ترکیب نواحی مشابه تعداد 35 ناحیه تنوع پر تکرار در ژنوم گوسفند و 141 مورد در ژنوم گاو شناسایی شد، فقط 20 درصد نواحی CNV در ژنوم نژاد­های گوسفند با ژنوم گاو هلشتاین همپوشانی داشت. علاوه بر این، نواحی این تنوع در ژنوم گوسفند با 40 ژن مرجع و 62 QTLدر ژنوم گاو، و 110 ژن مرجع در ژنوم انسان بطور کامل یا جزئی همپوشانی داشت. توزیع تنوع­ها در هر دو گونه در نواحی ساب­تلومریک و پری سانترومریک بیشتر بود. بر اساس این نتایج، شاید بتوان از روی پیوستگی این تنوع با نواحی ژنی در ژنوم گاو ژن­ها و QTL­های ژنوم گوسفند را شناسایی نمود. برای کسب نتایج دقیق­تر مطالعات بیشتری نیاز است.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of copy number variation profile in sheep with Holstein breed

نویسنده [English]

  • Maryam Nosrati

چکیده [English]

The aim of this study was to compare copy number variation (CNV) in some Italian sheep breeds with Holstein cows. Blood samples were collected form 580 Holstein bulls and 360 different Italian sheep breeds and then DNA was extracted. The SNPs genotypes across the genome were determined by ovine and bovine 50K BeadChip in both species. By PennCNV, the 904 CNV with mean and median size of 154.7 kb and 103.7 kb in sheep and 744 CNV with mean and median of 213.7 kb and 124.5 kb in Holstein were detected, respectively. A total length of this variation were 8.5 Mb in sheep and was 80 Mb in cattle. After merging similar regions, the 35 and 141 non- unique copy number variation regions (CNVR) were detected in sheep and cows, respectively which 20% of sheep’s CNVRs overlapped with cattle’s CNVRs. In addition to, these regions in Sheep genome were partially or completely overlapped with 40 RefGen & 62 QTL in cattle and 110 RefGen in Human. CNVRs distribution in both species was more in subtelomeric and pericentromeric regions. According to these results, it could be possible to use bovine CNVRs which were closely linked to genes for characterizing functional genes and QTLs in sheep, however more studies are need in this regards.

کلیدواژه‌ها [English]

  • CNV
  • Comparative genome profile
  • Genome variation
  • Holstein cows
  • sheep

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