مقایسه تأثیر آنتی اکسیدانی عصاره برگ زیتون با ویتامین های C و E بر شاخص های کیفی اسپرم گاو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد فیزیولوژی دام

2 عضو هیات علمی دانشگاه لرستان

3 عضو هیآت علمی دانشگاه لرستان

4 عضو هیأت علمی

چکیده

در این تحقیق اثر آنتی اکسیدانی عصاره برگ زیتون در مقایسه با ویتامین­های C و E در اسپرم سرد شده گاو در طی مدت   48ساعت نگهداری در شرایط دمایی پنج درجه
سانتیگراد مقایسه شد. اسپرم از قسمت دم اپیدیدیم بیضه 10رأس گاو نر نژاد هولشتاین دورگ با میانگین سنی سه تا پنج سال جمع­آوری و با افزودن رقیق­کننده تخم­مرغ و
سیترات سدیم حاوی عصاره برگ زیتون (در سه غلظت نیم، یک و دو میلی­گرم در میلی­لیتر) و ویتامین­های E  و C (هرکدام با غلظت دو میلی­گرم در میلی­لیتر) رقیق شدند. افزودن
عصاره برگ زیتون به رقیق­کننده، تحرک اسپرم را کاهش داد (05/0P<). سلامت غشا اسپرم گاو استفاده از ویتامین E بالاتر از سایر تیمارها بود (05/0P<). میزان پراکسیداسیون چربی­ها در تیمار حاوی دو میلی گرم در میلی لیتر عصاره برگ زیتون کمتر از سایر تیمارها بود (05/0P<). نتایج این تحقیق نشان داد که افزودن عصاره برگ زیتون به رقیق­کننده اسپرم در طی مدت 48 ساعت نگهداری در شرایط دمایی پنج درجه سانتیگراد علیرغم کاهش میزان اکسیداسیون لیپیدی موجب کاهش تحرک اسپرم گاو می­شود و
بنابراین ترکیب مناسبی برای استفاده در رقیق­کننده­های اسپرم گاو نمی­باشد.  

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of the antioxidant effect of olive leaf extract with vitamins C and E on bovine sperm quality

نویسنده [English]

  • Arash Kheradmand 3
چکیده [English]

In this study antioxidant effects of olive leaf extract (OLE) in comparison to vitamin C and E on chilled-stored (at 5 °C) bovine sperm was evaluated. The sperm samples collected from epididymis of ten Holstein bovine testis with 3-5 years old, and were diluted with egg yolk and sodium citrate containing 0.5, 1.0 and 2.0 mg/ml of OLE as well as 2 mg/ml vitamin E and C. Addition of OLE significantly reduced sperm motility (P<0.05). Vitamin E significantly improved sperm membrane integrity compared to the other groups (P<0.05). TBARS in 2 mg/ml of OLE treatment was significantly less than that in other treatments (P<0.05). The results showed that addition of OLE to bovine sperms stored at 5 ͦ C for 48 h negatively affected sperm motility despite of a decrease in lipid peroxidation, and therefore, it is not suitable extender to be used for bovine spermatozoa.

کلیدواژه‌ها [English]

  • Bovine
  • Lipid Peroxidation
  • Oleuropein
  • Sperm
  • Thiobarbituric acid reactive substances
Alirezaei M, Kheradmand A, Heydari R, Tanideh N, Neamati Sh and Rashidipour, M (2012a) Oleuropein protects ethanol-induced oxidative stress and modulates sperm quality in the rat testis. Mediterranean Journal of NutritionandMetabolism 5: 205–211.
Alirezaei M, Dezfoulian O, Neamati Sh, Rashidipour M, Tanideh N and Kheradmand A (2012b) Oleuropein prevent ethanol-induced gastric ulcers via elevation of antioxidant enzyme activities in rats. JournalofPhysiologyandBiochemistry 68: 583-592.
Alirezaei M, Dezfoulian O, Kheradmand A, Neamati Sh, Khonsari A and Pirzadeh A (2012c) Hepatoprotective effects of purified oleuropein from olive leaf extract against ethanol- induced damages in the rat. Iranian Journal of Veterinary Research 13: 3- 40.
Andrreadou I, Iliodromitis EK, Micors E, Constantinou M, Agalias A and Magiatis P (2006) The olive constituent oleuropein exhibits anti-ischemic,antioxidative, and hypolipitemic effects in anesthetized rabbits. Journal of Nutrition 136: 13-19.
Bilodeau JF, Chatterjee S, Sirard MA and Gagnon C (2000) Levels of antioxidant ‎defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. ‎Journal of  Molecular and Morphological Review 55: 282–288.
Esmaeili-Mahani S, Rezaeezadeh-Roukerd M, Esmaeilpour  K, Abbasnejad  M, Rasoulian B, Sheibani V, Kaeidi A and Hajializadeh Z  (2010) Olive (Olea euoropaea L.) Leaf extract elicits antinociceptive activity, potentiates morphine analgesia and suppresses morphine hyperalgesia in rats. Journal of Ethnopharmacology 132: 200-205.
Jalali SM, Mohammady M and Roostaaei M (2013) Effect of Olive leaf extract on lipid peroxidation male sperm during storage at 4 ºC. National Congress of NewTechnologies in Animal Sciences. 20 -21 Nov., Islamic Azad University, Isfahan. Iran.
Khanjani Jelodar S and Bigdeli, M R (2013) Effect of Olive Leaf Extract on the behavioral signs of huntington's disease and antioxidant enzymatic activity in the rat brain.  Physiology and Pharmacology 17: 328-338.
Kumar S, Biswas S, Mandal D, Roy HN, Chakraborty S, Kabir SN, Banerjee S and Mondal NB (2007) Chenopodium album seed extract: a potent sperm-immobilizing agent both in vitro and in vivo. Contraception 75: 71– 78.
Lujan RJ, Rodriguez JML and Castro MDL (2006) Dynamic ultrasound-assisted extraction of oleuropein and related biophenols from olive leaves. Journal of Chromatography 108: 76-82.
Maldjian A, Pizzi F, Gliozzi T, Cerolini S, Penny P and Noble R (2005) Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology 63: 411-421.
Malo L, Gil R, Cano F and Martínez I (2011) Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology 75: 1735–1741.
Mohame R, Pineda M, and Aguilar M (2007) Antioxidant capacity of extracts from wild and crop plants of mediterranean region. Journal of Food Science 72: 59-63.
Najafizadeh P, Dehghani F, Panjehshahi M and Hamzei Taj S (2013)The effect of a hydro-alcoholic extract of olive fruit on reproductive argons in male sprague-dawley rat. Iranian Journal of Reproduction Medicine 11: 293-300.
O'Flaherty C, de Lamirande E and Gagnon C (2006) Reactive oxygen species modulate independent protein phosphorylation pathways during human sperm capacitation.  Free Radical Biology and Medicine 40: 1045-1055.
Rafiei1 Z, Jafari SM, Alami M and Khomeiri M (2012) Evaluation of antimicrobial activity of olive leaf extracts by ELISA method. Iranian Journal of Medicinal and Aromatic Plants 28: 12-22.
Ranalli A, Contento S, Lucera L, Di Febo M, Marchegiani D and Di Fonzo V (2006) Factors affecting the contents of iridoidoleuropein in olive leaves (Olea europaea L.). Agricultural and Food Chemistry 54: 434-40.           
Sharma RK and Agarwal, A (1996) Role of reactive oxygen species in male infertility. Urology 48: 835-850.
Sherif W, Mansour I, Sibghatullah S, Sree H, Mueen A and Khaleel ARN (2013) Sensibility of male rats’ fertility against olive oil, Nigella sativa oil and pomegranate extract. Asian Pacific Journal of Allergy and Immunology3: 563-568.
Sliwa L and Macura B (2005) Evaluation of cell membrane integrity of spermatozoa by hypoosmotic  swelling test - "water test" in mice after intra peritone aldaidzein administration. Archives of Andrology 51: 443-448.  
Sonmez M, Turk G and Yuce A (2005) The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and male Wistar rats. Theriogenology 63(7):2063-72. 
Subbarao KV, Richardson JS and Ang LC (2010) Autopsy samples of Alzheimer's cortex show increased peroxidation in vitro. Journal of Neurochemistry 55: 342-345.