نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانش آموخته کارشناسی ارشد رشته تغذیه طیور دانشگاه آزاد شهرقدس
2 عضو هیئت علمی دانشگاه آزاد اسلامی، واحد شهر قدس
3 عضو هیئت علمی دانشگاه تربیت مدرس
چکیده
جهت بررسی تأثیر استفاده از اسانس رزماری در جیره، بر فراسنجههای کیفی اسپرم، آزمایشی با استفاده از 16 قطعه خروس راس 308 مادر گوشتی در قالب طرح کاملاً تصادفی با 4 تیمار و 4 تکرار، در سن 24هفتگی انجام شد. تیمارهای آزمایشی شامل جیرههایی با سطوح صفر، 50، 100 و 200 میلیگرم اسانس رزماری در هر کیلوگرم خوراک بود. اسپرمگیری در روزهای صفر، 14، 28، 42 و 56 آزمایش انجام گرفت. نتایج نشان داد صفات دامنه جابجایی سر اسپرم، سرعت اسپرم در مسیر مستقیم و میانگین سرعت در مسیر مستقیم در روزهای 42 و 56 نمونهگیری و صفات درصد خطی بودن جنبایی اسپرم، فعالیت غشاء، درصد کل اسپرم متحرک و زندهمانی در روز 56 نمونهگیری در تیمار سه (100 میلیگرم اسانس رزماری) بهطور معنیداری افزایش و تعداد اسپرمهای مرده کاهش یافت (05/0P<). در روزهای مختلف نمونهگیری بین تیمارها تفاوت معنیداری از نظر غلظت اسپرم، سطح مالوندیآلدهید، آپوپتوزیس، تغییرات مورفولوژیک، راستی مسیر طی شده، سرعت در مسیر منحنی و درصد اسپرمهای دارای حرکت پیشرونده مشاهده نشد. براساس نتایج حاصل، استفاده از 100 میلیگرم اسانس رزماری در جیره، در مقایسه با سایر تیمارها سبب بهبود برخی فراسنجههای کیفی اسپرم شده است.
کلیدواژهها
عنوان مقاله [English]
Effects of dietary rosemary essential oil on semen quality of broiler breeder roosters
نویسندگان [English]
- behrooz shokrani 1
- morteza mehri 2
- amir fattah 2
- mohsen sharafi 3
- fatemeh shirmohammad 2
1
2
3
چکیده [English]
Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates per each group. Semen samples were collected on days 0, 14, 28, 42 and 56 of experiment. Results showed that 100mg rosemary significantly improved several traits of sperm such as Amplitude of Lateral Head Displacement (ALH), Average Path Velocity (VAP), and Straight Line Velocity (VSL) on days 42 and 56, and sperm Membrane Integrity (MI), sperm viability, Linearity (LIN) and Total Motility (TM) on day 56 (P<0.05) and significantly decreased dead sperms (P<0.05). However, sperm concentration, apoptosis and morphological changes, Sperm Track Straightness (STR), Curvilinear Velocity (VCL), Progressive motility (PM) and malondialdehyde (MDA) level were not affected by experimental treatment. It can be concluded that 100 mg rosemary essential oil/kg of diet could be efficient for improvement of some qualitative sperm parameters.
کلیدواژهها [English]
- Apoptosis
- Malondialdehyde
- Morphology
- Motility
- Sperm
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