نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد رشته تغذیه طیور دانشگاه آزاد شهرقدس

2 عضو هیئت علمی دانشگاه آزاد اسلامی، واحد شهر قدس

3 عضو هیئت علمی دانشگاه تربیت مدرس

چکیده

جهت بررسی تأثیر استفاده از اسانس رزماری در جیره‌، بر فراسنجه­های کیفی اسپرم، آزمایشی با استفاده از 16 قطعه خروس راس 308 مادر گوشتی در قالب طرح کاملاً تصادفی با 4 تیمار و 4 تکرار، در سن 24هفتگی انجام شد. تیمارهای آزمایشی شامل جیره‌هایی با سطوح صفر، 50، 100 و 200 میلی­گرم اسانس رزماری در هر کیلوگرم خوراک بود. اسپرم­گیری در روزهای صفر، 14، 28، 42 و 56 آزمایش انجام گرفت. نتایج نشان داد صفات دامنه جابجایی سر اسپرم، سرعت اسپرم در مسیر مستقیم و میانگین سرعت در مسیر مستقیم در روزهای 42 و 56 نمونه­گیری و صفات درصد خطی بودن جنبایی اسپرم، فعالیت غشاء، درصد کل اسپرم متحرک و زنده‌مانی در روز 56 نمونه­گیری در تیمار سه (100 میلی­گرم اسانس رزماری) به‌طور معنی‌داری افزایش و تعداد اسپرم­های مرده کاهش یافت (05/0P<). در روزهای مختلف نمونه­گیری بین تیمارها تفاوت معنی­داری از نظر غلظت اسپرم، سطح مالون­دی­آلدهید، آپوپتوزیس، تغییرات مورفولوژیک، راستی مسیر طی شده، سرعت در مسیر منحنی و درصد اسپرم­های دارای حرکت پیش­رونده مشاهده نشد. براساس نتایج حاصل، استفاده از 100 میلی­گرم اسانس رزماری در جیره، در مقایسه با سایر تیمارها سبب بهبود برخی فراسنجه­های کیفی اسپرم شده است.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of dietary rosemary essential oil on semen quality of broiler breeder roosters

نویسندگان [English]

  • behrooz shokrani 1
  • morteza mehri 2
  • amir fattah 2
  • mohsen sharafi 3
  • fatemeh shirmohammad 2

چکیده [English]

Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates per each group. Semen samples were collected on days 0, 14, 28, 42 and 56 of experiment. Results showed that 100mg rosemary significantly improved several traits of sperm such as Amplitude of Lateral Head Displacement (ALH), Average Path Velocity (VAP), and Straight Line Velocity (VSL) on days 42 and 56, and sperm Membrane Integrity (MI), sperm viability, Linearity (LIN) and Total Motility (TM) on day 56 (P<0.05) and significantly decreased dead sperms (P<0.05). However, sperm concentration, apoptosis and morphological changes, Sperm Track Straightness (STR), Curvilinear Velocity (VCL), Progressive motility (PM) and malondialdehyde (MDA) level were not affected by experimental treatment. It can be concluded that 100 mg rosemary essential oil/kg of diet could be efficient for improvement of some qualitative sperm parameters.

کلیدواژه‌ها [English]

  • Apoptosis
  • Malondialdehyde
  • Morphology
  • Motility
  • Sperm
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