پویش ژنومی سندرم آسیت در نسل F2 حاصل از تلاقی مرغ بومی آذربایجان غربی و لاین گوشتی آرین

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجو دکتری دانشکده کشاورزی تربیت مدرس - عضو هیات علمی موسسه تحقیقات علوم دامی کشور بخش بیوتکنولوژی

2 معاون آموزشی دانشکده کشاورزی تربیت مدرس

3 استادیار گروه علوم دامی دانشکده کشاورزی تربیت مدرس

چکیده

سندرم آسیت بیماری متابولیکی پرهزینه‌ای است که به دلیل انتخاب شدید برای سرعت رشد وکاهش ضریب تبدیل غذاییدر طیور اتفاق می‌افتد. این سندرم یکی از مهم‌ترین عوامل مرگ‌ومیر در صنعت جوجه گوشتی است و عامل خسارات قابل‌توجه اقتصادی و کاهش آسایش حیوان است. در این تحقیق به ‌منظور یافتن ژن و نواحی ژنومی مؤثر بر سندرم آسیت، مطالعه پویش کل ژنوم (GWAS) با استفاده از یک تراشه SNP 60k ژنوم مرغ در 101پرنده نسل F2 حاصل تلاقی دوطرفه مرغ بومی آذربایجان غربی و سویه گوشتی آرین انجام شد. از روش آماری بهترین پیش­بینی نا اریب خطی (GBLUP) جهت بررسی ارتباط SNP ها با صفات مرتبط با آسیت استفاده شد. بر روی کروموزم هفت چندین SNP برای صفت نسبت بطن راست به‌ کل بطن به ‌عنوان شاخص آسیت، به سطح احتمال پیشنهادی (5-10 × 253/8) رسیدند.دو SNP پیشنهادی در داخل ژنCCDC141 و OSBPL6 واقع شدند که پیشتر به ‌عنوان ژن‌های مؤثر بر نارسائی‌های قلبی در انسان گزارش شده بودند. این ناحیه ژنومی (14602723- 12745561) بر روی کروموزوم هفت شامل ژن‌های متعددی است که در انسان بر روی هایپرتروفیعضله قلب، ضربان قلب و بیماری‌های قلبی تأثیر دارند. انتخاب برای مقاومت به آسیت در جوجه‌های گوشتی با استفاده از این یافته‌ها، باعث تسریع در پیشرفت ژنتیکی خواهد شد.

کلیدواژه‌ها


عنوان مقاله [English]

Genome wide association study for Ascites Syndrome in F2 crosses of Azerbaijan native fowls and Arian broiler line

نویسندگان [English]

  • Hossein Emrani 1
  • Rasoult Vaes Torshizi 2
چکیده [English]

Ascites Syndrome is a costly metabolic disorder that has been linked to intense selection on growth rate and low feed conversion ratio in broilers. This Syndrome has become one of the most frequent causes of mortality within the broiler industry and leads to substantial economic losses and reduced animal welfare. To identify genes and genomic regions associated with Ascites Syndrome, genome wide association study was performed by the chicken 60K SNP panel on 101 birds from a F2 chicken population derived from the reciprocal cross between Arian broiler Line and Azerbaijan native fowls. Genomic best linear unbiased prediction procedure was used to calculate associations between each SNP and ascite related traits. Several SNPsfor the ratio of right ventricular to total ventricular, an indicator for Ascites syndrome, reached the suggestive threshold (8.25×10-5) on chromosome 7. Two SNPs detected within CCDC141 and OSBPL6 genes were previously reported for human heart disorder. There are a large number of genes in this region (12745561-14602723) on chicken chromosome 7 that are related to hypertrophic cardiomyopathy, heart rate and human heart disorder. Selection for ascites resistance in broiler chicken using these findings could accelerate the genetic progress.

کلیدواژه‌ها [English]

  • Ascites Syndrome
  • Candidate Gene
  • chicken
  • GWAS
  • Ventricular
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