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اخبار و اعلانات

اثرات ژله رویال بر بیان ژن های کدکننده آنزیم های آنتی اکسیدان در بلوغ و تولید برون تنی رویان بز

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 دانشگاه علوم کشاورزی و منابع طبیعی ساری، تخصص: فیزیولوژی دام نر و ماده/ تولیدمثل دام

چکیده

اثر غلظت های مختلف ژله رویال به عنوان یک آنتی اکسیدان، بر بلوغ برون تنی تخمک بز در یک طرح کاملا تصادفی با چهار تیمار ( غلظت های صفر (شاهد)، پنج، 10 و 20 میلی گرم بر میلی لیتر ژله رویال) بررسی شد. تخمدان های بز از کشتارگاه جمع آوری و مجموعه اووسایت کومولوس بز از فولیکول های آنترال جدا شدند و در محیطMedium 199 به همراه غلظت های مختلف ژله رویال کشت داده شدند. اووسایت ها پس از ۲4 ساعت به بلوغ رسیدند. نتایج نشان داد که افزایش غلظت ژله رویال به طور معنی داری ( 05/0P< ) نرخ بلوغ برون تنی تخمک ها را از 54 درصد (تیمار شاهد) به 93 درصد (تیمار20 میلی گرم در میلی لیتر) افزایش داد. افزایش غلظت ژله رویال در محیط کشت بلوغ، موجب افزایش معنی دار ( 05/0P< ) نرخ کلیواژ از 64/2±5/62 درصد (تیمار شاهد) به 78/3±6/85 درصد (تیمار 20میلی گرم در میلی لیتر) و نرخ تولید بلاستوسیت از 26/2±4/15 درصد (تیمار شاهد) به 42/3±6/38 درصد (تیمار20 میلی گرم در میلی لیتر) شد. با افزایش غلظت ژله رویال در محیط، بیان نسبی ژن های سوپراکسید دیسموتاز و گلوتاتیون پراکسیداز و کاتالاز به طور معنی داری (05/0P< ) افزایش یافت. بر اساس نتایج حاصل، افزودن 20 میلی گرم بر میلی لیتر ژله رویال در محیط بلوغ تخمک، موجب بهبود وضعیت اکسیداتیو، بلوغ هسته ای تخمک و رشد و نمو بلاستوسیست بز شد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of royal jelly on gene expression of antioxidant enzymes in in vitro maturation and embryo development of goat oocytes

چکیده [English]

This research was conducted to investigate the effect of different concentrations of royal jelly (RJ) as an antioxidant source on in vitro maturation of goat oocytes. Goat ovaries were collected from a local abattoir. Cumulus-oocyte-complexes (COCs) were recovered from antral follicles. The COCs were placed in maturation medium and were reached to MII stage after 24 hours. The results indicated that the increasing of royal jelly concentration in the maturation media was significantly increased maturation rate of oocytes from control (54 ± 0%) to 20 mg/mL group (93 ± 0.5%). Also, the higher RJ concentrations were associated with increased cleavage (62.5 ± 2.64% to 85.6 ± 3.78%) and blastocyst rate (15.4 ± 2.26% to 38.6 ± 3.42%) from the RJ0 to the RJ20 group. Addition of RJ was significantly (P<0.05) increased the antioxidant gene expressions in the oocytes. In conclusion, supplementation of 20 mg/mL royal jelly to the maturation media was significantly improved the redox state, nuclear maturation and blastocyst formation.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • Embryonic development
  • Goat oocytes
  • in vitro maturation
  • Royal jelly
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تولیدات دامی
دوره 18، شماره 4
دی 1395
صفحه 867-876
فایل ها
هم رسانی
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