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شناسایی چندشکلی گیرنده یک ژن دوپامین در مرغ بومی خوزستان با استفاده از روش PCR-RFLP

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، دانشکده‏ علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

2 استاد، دانشکده‏ علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

3 دانشیار، دانشکده‏ی علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

چکیده

این پژوهش به منظور بررسی چند­شکلی گیرنده­ی یک ژن دوپامین با استفاده از روش PCR-RFLP در مرغ بومی خوزستان انجام شد. به طور تصادفی از 100 قطعه مرغ بومی خوزستان خون گیری شد وDNA نمونه‏ها با استفاده از روش نمکی استخراج شد. واکنش زنجیره‏ای پلیمراز برای تکثیر قطعه 283 جفت باز(گیرنده­ی یک) ژن دوپامین به کمک آغازگرهای اختصاصی انجام و قطعه تکثیر شده به‏ وسیله آنزیم برشیCfrI هضم شد. فراوانی‏ آلل‏های G و Aبه ترتیب 39/0 و 61/0 و فراوانی ژنوتیپ‏های AA، GGو AG به ترتیب 40/0، 18/0 و 42/0بود. بر اساس نتایج آزمون مربع کای فراوانی ژنوتیپ‏ها در این جایگاه ژنی در حالت تعادل هاردی-وینبرگ نبودند. با تعیین توالی دو جهش در بازهای 123 و 198، (به ترتیب از نوع A به G و C به T) مشاهده شد. نتایج حاصل از توالی یابی مشخص کرد که آنزیم برشی CfrI ، به درستی جهش­ها را شناسایی می­کند.

کلیدواژه‌ها

عنوان مقاله [English]

Association of single nucleotide polymorphism of STAT1 gene with milk production traits in Brown Swiss cattle

نویسندگان [English]

  • Maryam Bazgiri 1
  • Mohammad taghi Beigi nassiri 2
  • Jamal Fayazi 3

1 M.Sc. Graduated, Faculty of Animal Science and Food Technology, Ramin University of Agriculture and Natural Resources of Khouzestan

2 Professor,Faculty of Animal Science and Food Technology, Ramin University of Agriculture and Natural Resources of Khouzestan

3 Associate Professor, Faculty of Animal Science and Food Technology, Ramin University of Agriculture and Natural Resources of Khouzestan

چکیده [English]

This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety-eight cows were collected randomly from tail vein. The DNA was extracted with Cinagene kit (PR881612C). Fragment of 314 bp in 3'UTR of STAT1 gene was amplified by PCR and digested by Pag1 restriction enzyme. Genotype frequencies were calculated by PopGene software (version 1.31). The SAS 9.1 software was used for statistical analysis and the mean of traits was compared by Duncan test. The genotype
frequencies of TT, TC and CC were 0.23, 0.25 and 0.52, respectively. The T and C allele frequency were 0.355 and 0.645, respectively. The expected heterozygosity was estimated 0.458. Result of Chi-square showed that this locus was not in the Hardy Weinberg equilibrium (P<0.05). The means of traits for milk, fat percent and fat yield, and protein yield were not significant but means of protein percent was significant (P<0.05). The mean of milk, fat and protein yield traits for CC genotype were more than two other genotypes. The results of present study showed that T allele is known as minor frequency allele and C allele have positive effect on milk, fat and protein yield traits.

کلیدواژه‌ها [English]

  • Brown Swiss breed
  • Gene
  • Milk production traits
  • STAT1
مراجع
  1. Akira S (1999) Functional roles of STAT family proteins: Lessons from knockout mice.
  2. Stem Cells 17:138–146.
  3. Ashwell  MS, Heyen DW, Sonstegard  TS,  Van Tassell CP, VanRaden PM, Ron M, Weller JI and Lewin HA (2004) Detection of quantitative trait loci affecting milk production, health and reproductive traits in Holstein cattle. Journal of Dairy Science 87:468–475.
  4. Band M R, Larson  J H, Rebeiz M, Green C A, Heyen D W, Donovan J, Windish R, Steining C, Mahyuddin P, Womack JE and Lewin H A (2000) An ordered comparative map of the cattle and human genomes. Genome Research 10:1359–1368.
  5. Bole-Feysot C, GoffinV, Edery M, Binart N and Kelly PA (2005) Prolactin (PRL) and its receptor: Actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocrin Reviews. 19:225-268
  6. Boutinaud M and Jammes H (2004) Growth hormone increases Stat5 and Stat1 expression in lactating goat mammary gland: A specific effect compared to milking frequency.Domestic Animal Endocrinology 27:363–378.
  7. Brym P, Kaminski S and.Ruoeæ A (2004) New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black and White and Jersey cattle. Journal of Applied Genetics 45: 445-452.
  8. Cobanoglu O,. Zaitoun I, Chang Y M, Shook G E and Khatib H (2006) Effects of the signal transducer and activator of transcription1 (STAT1) gene on milk production traits in Holstein dairy cattle. Journal of Dairy Science 89:4433-4437.
  9. Darnell J E (1997) STATs and gene regulation. Science 277:1630–1635.
  10. De Koning D (2006) Conflicting candidates for cattle QTLs. Trends Genetic 22: 301-305.
  11. Khatib H, Huang W, Mikheil D, Schutzkus V and.Monson L (2009) Effects of signal transducer and activator of transcription (STAT) genes STAT1and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle. Journal of Dairy Science 92: 6186–6191.
  12. Khatkar M.S, Thomson P.C, Tammenand I and.Raadsma H W (2004) Quantitative trait loci mapping in dairy cattle: Review and meta-analysis. Genetic Selectin Evolution 36: 163-190.
  13. Kumar M, Vohra V, Ratwan P and Chakravarty A K (2015) Exploring polymorphism in 3' UTR region of STAT1 gene in different Buffalo breeds. Indian Journal of Dairy Science 68(5): 473-476.
  14. Li C, Sun D, Zhang S, Wang S, Wu X, Zhang Q, Li Y and Qiao L (2014) Genome Wide Association Study Identifies 20 Novel Promising Genes Associated with Milk Fatty Acid Traits in Chinese Holstein. PLoS ONE 9(5): e96186.
  15. Mackinnon M J and.Georges M A J (1998) Marker assisted pre selection of young dairy sires prior to progeny-testing. Livestock Production Science 54: 229-250.
  16. Pollak E J (2005) Application and impact of new genetic technologies on beef cattle breeding: a ‘real world’ perspective. Australian Journal of Experimental Agriculture 45: 739-748.
  17. Rychtarova J, Sztankoova Z, Kyselova J, Zink V, Stipkova M, Vacek M and. Stolc L (2014) Effect of DGAT1, BTN1A1, OLR1, and STAT1 genes on milk production and reproduction traits in the Czech Fleckvieh breed. Czech Journal of Animal Science 59(2): 45–53
  18. SAS (2005). Statistics analysis system user’s Guide. Release 9.1. SAS Institute Inc., Cary, North Carolina, USA.
  19. Soller M (1994) Marker assisted selection-An overview. Animal Biotechnology 3: 193-201.
  20. Stewart W C, Morriso R F, Young S L and Stephens J M (1999) Regulation of signal transducers and activators of transcription (STATs) by effectors of adipogenesis: Coordinate regulation of STATs 1, 5A, and 5B with peroxisome proliferator-activated receptor- γ and C/AAAT enhancer binding protein-α. Biochimica et Biophysica Acta 1452:188–196.
  21. Tucker HA (2000) Hormones, mammary growth, and lactation: A 41-year perspective. Journal of. Dairy Science. 83: 874-884
  22. Watson CJ. (2001) STAT transcription factors in mammary gland development and tumor genesis. Journal of Mammary Gland Biology and Neoplasia 6: 115–127.
  23. Yeh F C and Yong R C (1990) PopGene version 1.31.University of Alberta.
  24. Yonekura S, Miyazaki H and Tokutake Y (2015) Comparative Expression Profiling of Lactogenic Hormone Receptor and It’s Signaling Molecules of Bovine Mammary Glands during lactation. Open Journal of Animal Sciences, 5: 106-113
  25.  Zimin A V, Delcher A L, Florea L, Kelley D R, Schatz M C, Puiu D, Hanrahan F, Pertea G, Van Tassell C P, Sonstegard T S,  Marçais G, Roberts M, Subramanian P, Yorke J A and Salzberg S L (2009) A whole-genome assembly of the domestic cow, Bos Taurus. Genome Biology:10(4): R42.

تولیدات دامی
دوره 19، شماره 1
اردیبهشت 1396
صفحه 13-22
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هم رسانی
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