تجزیه و تحلیل پروموتر ژن‏های مؤثر در تعادل منفی انرژی در گاوهای شیری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران، پاکدشت، ایران

2 استادیار، گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران، پاکدشت، ایران

چکیده

این تحقیق به‌منظور درک بهتر سازوکارهای تنظیمی بیان ژن‏های مربوط به تعادل منفی انرژی انجام شد. ژن‏های با بیان بالا در تعادل منفی انرژی با استفاده از داده‏های ریزآرایه و RNA-seq شناسایی و توالی‏های پروموتری آنها برای شناسایی فاکتورهای رونویسی جدید در ارتباط با این مراحل تجزیه و تحلیل شدند. افزون بر این، از پایگاه اطلاعاتی STRING برای ترسیم شبکۀ ژنی مرتبط با فاکتورهای رونویسی شناسایی‌شده در تعادل منفی انرژی استفاده شد. نتایج تجزیه و تحلیل بیان ژن‏ها نشان داد که هشت ژن بیان بالا و معنادار در مقایسه تعادل منفی انرژی شدید نسبت به خفیف دارند (05/0< P). درنتیجۀ تجزیه و تحلیل پروموتری این ژن‏ها، 19 فاکتور رونویسی شناسایی شد. این مجموعه مشتمل بر یک فاکتور رونویسی (NF-κB) با نقش تأییدشده در تعادل منفی انرژی و فاکتورهای رونویسی جدید همچون SP1، ZBP89، NFI، Zf9، MYC، ZBTB7A، FOXF2، و KLF6، که نقش تنظیمی آنها در تعادل منفی انرژی گزارش نشده، است. براساس نتایج، 18 فاکتور رونویسی کاندید جدید معرفی‌شده در این مطالعه می‏توانند اطلاعات جدیدی را در درک بهتر شبکۀ تنظیمی مؤثر در تعادل منفی انرژی فراهم کنند.

کلیدواژه‌ها


عنوان مقاله [English]

Promoter analysis of effective genes in negative energy balance in dairy cows

نویسندگان [English]

  • Zohreh Mozduri 1
  • Mohammad Reza Bakhtiarizadeh 2
1 M.Sc. Student, Department of Animal and Poultry Sciences, College of Aboureihan, University of Tehran, Tehran, Iran
2 Assistant Professor, Department of Animal and Poultry Sciences, College of Aboureihan, University of Tehran, Tehran, Iran
چکیده [English]

This study was done to gain insights into transcriptional regulation of negative energy balance (NEB) assoctiated genes. Overexpressed genes in NEB were identified using microarray and RNA-seq data and promoter analysis of these overexpressed genes was applied to identify novel transcription factors. Moreever, STRING database was used to construct a regulatory network of identified transcription factors. The results of the gene expression analysis revealed that eight genes in severe NEB are more frequent and significant (P<0.05) in comparison to the mild NEB. Promoter analysis showed that promoters of overexpressed genes are enriched in putative binding sites for 19 transcription factors. This group included known NEB-associated transcription factor (NF-κB), and a number of transcription factors (such as SP1, ZBP89, NFI, Zf9, MYC, ZBTB7A, FOXF2 and KLF6) that had not been previously reported to be associated with NEB. Based on the present results, 18 new effective candidate trsnacription factors introduced in this study can provide new information to gain a better understanding of the regulatory network involved in NEB.

کلیدواژه‌ها [English]

  • gene expression
  • gene network
  • Microarray
  • RNA-Seq
  • transcription factors
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