پویش‌ کل ژنوم هشت نژاد گاو بومی ‌ایران برای شناسایی نشانه های ‌انتخاب

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران

2 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران

3 استاد، گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران

چکیده

در تحقیق حاضر، قسمت‌هایی از ژنوم گاوهای بومی ایران که شواهدی از انتخاب برای جهش‌های مختلف را نشان می‌دهند، شناسایی شد. بدین منظور از داده‌های 777962 نشانگر چندشکلی ‌تک‌نوکلئوتید پراکنده در سرتاسر ژنوم و 90 حیوان از هشت نژاد گاو بومی ایران (سرابی، کرمانی، سیستانی، نجدی، کردی، تالشی، پارس و مازندرانی) برای شناسایی نشانه‌های انتخاب استفاده شد. بررسی تمایز جمعیتی با استفاده از روش شاخص‌ تثبیت (Fst) تصحیح شده برای اندازه نمونه (θ) نشان داد که در چندین مکان ژنی شواهدی از انتخاب در این هشت نژاد وجود دارد. در طول 30 کروموزوم گاو، تعداد هفت ناحیه ژنومی شناسایی شد که نشانه‌های انتخاب در آن‌ها وجود داشت. این نواحی بر روی کروموزوم‌های یک، دو، هفت، هشت، 12، 13 و X واقع بودند. بررسی ژن‌ها و QTL های پیشین گزارش شده در مکان‌هایی از ژنوم گاو که تحت تأثیر انتخاب بوده‌اند، نشان داد که این مکان‌ها با ژن‌ها و QTL های صفات مهم اقتصادی نظیر صفات مرتبط با تولید و اجزای شیر، وزن بدن، مقاومت به سرما و صفات تولیدمثل مرتبط هستند. نتایج تحقیق حاضر، اطلاعات مفیدی از وجود تنوع ژنتیکی و نشانه های انتخاب در گاوهای بومی ایران فراهم کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Whole-genome scan of eight Iranian native cattle breeds to detect selection signatures.

نویسندگان [English]

  • Nader Forough Ameri 1
  • Masoud Asadi Foozi 2
  • Ali Esmailizadeh Koshkoiyeh 3
1 Ph.D. Student, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
3 Professor, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده [English]

This study aimed to identify genes that show signatures of selection for mutations in Iranian native cattle breeds. The data consisted of genotypes for ~777962 SNP markers of 90 animals from eight Iranian native cattle breeds distributing over the country. Therefore, the aim was to identifying divergently selected regions of the genome. Study of population differentiation across the genome using Weir and Cockerham’s FST test revealed some regions showing evidence of selection. Across the 30 bovine chromosomes (BTA), seven putative selection signatures were detected. These regions were located on chromosomes 1, 2, 7, 8, 12, 13 and X. Finally, study of the reported QTL regions in the orthologous areas of the cattle genome showed that the genomic regions identified in this study overlapped with the reported QTL representing economically important traits such as milk yield, cold tolerance and reproductive traits. Result of this research provided important information on existence of genetic diversity and selection signatures in Iranian native cattle.

کلیدواژه‌ها [English]

  • Key Words: Fixation index
  • Genomic scan
  • Iranian native cattle
  • Population differentiation
  • Selective sweeps
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