تأثیر آلبومین سرم گاوی (BSA) بر فراسنجه های جنبایی اسپرم و باروری خروس بومی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، اهواز، ایران

2 گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، اهواز، ایران

چکیده

هدف از انجام تحقیق حاضر، بررسی اثر آلبومین سرم گاوی (BSA) بر تحرک و باروری اسپرم خروس با استفاده از 20 قطعه خروس و 48 قطعه مرغ بومی بود. منی جمع­آوری شده از خروس­ها با هم مخلوط شدند. برای آزمایش اول، منی مخلوط پس از رقیق‌سازی به چهار قسمت تقسیم شد. تیمارها عبارت بودند از منی رقیق شده بدون BSA و دارای یک درصد BSA. در هر گروه، ارزیابی تحرک اسپرم طی 30 دقیق پس از اسپرم‌گیری و 24 ساعت پس از نگهداری منی در دمای چهار درجه سلسیوس انجام شد. ارزیابی فراسنجه­های تحرک اسپرم­ها با روش کامپیوتری (کاسا) و میکروسکوپی (روش معمول) انجام گرفت. برای آزمایش دوم، مرغ­ها به چهار گروه با 12 قطعه مرغ در هر گروه تقسیم شدند و منی رقیق شده تازه بدون BSA و دارای یک درصد BSA و نیز منی رقیق ذخیره شده 24 ساعته بدون BSA و حاوی یک درصد BSA را با تلقیح مصنوعی دریافت کردند. پس از انکوباسیون تخم­مرغ­ها، میزان تخم­های بارور در هر تیمار ثبت شد. سطح یک درصد BSA تأثیری بر تحرک اسپرم و باروری آن در منی تازه نداشت، اما 24 ساعت پس از نگهداری منی به حالت مایع، BSA باعث حفظ تحرک (50/71 در مقابل 62/28 درصد) و باروری (44/61 در مقابل 43/34 درصد) اسپرم­ها شد (05/0P<). براساس نتایج حاصل، افزودن BSA به منی رقیق شده، تحرک و باروری اسپرم خروس بومیرا پس از 24 ساعت نگهداری در دمای چهار درجه سلسیوس بهبود بخشید.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of bovine serum albumin on sperm motility parameters and fertility of indigenous rooster

نویسندگان [English]

  • Saleh Tabatabaei Vakili 1
  • Morteza Mamouei 2
1 Associate Professor, Department of Animal Science, Faculty of Animal and Food Sciences, Ramin Agriculture and Natural Resource University of Khouzestan, Ahwaz, Iran
2 Professor, Department of Animal Science, Faculty of Animal and Food Sciences, Ramin Agriculture and Natural Resource University of Khouzestan, Ahwaz, Iran.
چکیده [English]

In this Research, effect of bovine serum albumin (BSA) on motility and fertility of indigenous rooster spermatozoa was evaluated using 20 and 48 indigenous roosters and hens, respectively. Semen was collected and pooled. In the first experiment, the pooled semen was diluted and divided into four aliquots.The treatments were included the diluted semen without BSA and with 1% BSA; In each group, spermatozoa motility was evaluated during 30 minutes of semen collection and 24 hours after semen storage in 4°C. Spermatozoa motility parameters were evaluated by computer assisted sperm analysis and routine methods. In the second experiment, hens were divided into 4 groups with 12 hens in each group and fresh diluted semen without BSA and containing 1% BSA as well as stored diluted semen without BSA and containing 1% BSA were inseminated. The eggs were incubated and proportion of fertile egg in each treatment group was recorded. By adding 1% BSA into the diluted semen of indigenous rooster, sperm motility parameters and fertility rate were not affected in fresh semen. However, after 24 hours of semen storage in liquid condition, BSA significantly preserved motility and fertility potential of spermatozoa (P<0.05). In conclusion, BSA improved the spermatozoa motility and fertility of indigenous rooster in liquid storage for 24 hours.  

کلیدواژه‌ها [English]

  • Artificial insemination
  • BSA
  • fertility
  • indigenous chicken
  • reproduction
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