بهبود ذخیره سازی اسپرم خروس با استفاده از اسانس الکلی رزماری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجو کارشناسی ارشد، گروه علوم دامی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج - ایران

2 استادیار گروه علوم دامی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج - ایران

چکیده
هدف تحقیق حاضر بررسی تأثیر سطوح مختلف اسانس رزماری (صفر، 10، 5/12، 6/16، 25 و50 میلی‌گرم در لیتر) در رقیق‌کننده منی، بر کمیت و کیفیت اسپرم خروس، بعد از فرایند انجماد- یخ‌گشایی بود. از 10 قطعه خروس سویه تجاری راس در این مطالعه استفاده شد. نمونه‌های منی هفته‌ای دو بار جمع‌آوری و پس از افزودن مایع رقیق کننده بر پایه لستین به آنها در دمای پنج درجه سانتی‌گراد نگهداری شدند. رقیق کننده حاوی غلظت‌های 10 و 5/12 میلی‌گرم در لیتر اسانس رزماری، جنبایی کل و رقیق کننده حاوی غلظت‌های 10 ، 5/12 و 6/16 میلی‌گرم در لیتر اسانس رزماری، حرکت پیشرونده اسپرم‌ و نیز زنده‌مانی آنها قبل از انجماد را بهبود بخشید(05/0 p≤). کمترین و بیشترین تحرک اسپرم‌ها به ترتیب در رقیق کننده بدون رزماری و رقیق کننده حاوی 10 میلی‌گرم در لیتر از اسانس رزماری مشاهده شد(05/0 p≤). یکپارچگی غشاء سلولی اسپرم‌‌ها در رقیق کننده حاوی 10 میلی‌گرم در لیتر اسانس رزماری نسبت به سایر غلظت‌ها ( به جز غلظت‌ 5/12 میلی‌گرم در لیتر) افزایش یافت (05/0 p≤). آپوپتوز اسپرم ها در رقیق کننده حاوی 10، 5/12 و 6/16 میلی‌گرم در لیتر اسانس رزماری، کاهش یافت (05/0 p≤). بر اساس نتایج این تحقیق، اسانس رزماری در سطوح 10 و 5/12میلی‌گرم در لیتر در مایع رقیق‌کننده، کیفیت اسپرم خروس را پس از یخ‌گشایی بهبود می بخشد.

کلیدواژه‌ها


عنوان مقاله English

Improving sperm cryopreservation of rooster by using rosemary alcoholic essential oil

نویسندگان English

Hanie Shafigh 1
Malek Shakeri 2
Saeed Zeinoaldini 2
Hamid Kohram 2
Mehdi Zhandi 2
Morteza Moghbeli 2
1 M.Sc. Student, Department of Animal Science, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Assistant Professor, Department of Animal Science, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده English

The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain rooster were used. Semen samples were collected, 2 times a week. After adding the samples of semen based on the lecithin extender, they were placed and preserved at 5 ° C. The treatments of 10 and 12.5 mg/L of Rosemary significantly improved the mobility, in addition, treatments of 10, 12.5 and 16.6 mg/L improved progressive motility and viability before freezing (p≤ 0.05). The lowest and highest motility rates were respectively found in treatments of 0 and 10 mg/L of essential oil of rosemary (p≤ 0.05). Functional integrity of the sperm plasma membrane increased with treatment of 10 mg/L of essential oil of rosemary in comparison to other groups, except 12.5 mg/L. Also treatments of 10, 12.5 and 16.6 mg/L significantly decreased the apoptosis. According to these results, it seems that adding the levels of 10 and 12.5 mg/L rosemary extract’ in semen, based on lecithin extender can improve rooster’s sperm quality after thawing.

کلیدواژه‌ها English

Antioxidant
freezing-thawing
Rooster Sperm
Rosemary essential oil
semen extender
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