تأثیر نانوذرات روی بر کیفیت اسپرم و بازده آبستنی در گاو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران‌ـ‌ایران

2 استادیار گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران، پاکدشت‌ـ‌ایران

3 استادیار گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران‌ـ‌ایران

4 دانشجوی دکتری فیزیولوژی دام، دانشکده کشاورزی، دانشگاه تبریز

5 دکتری تخصصی، شرکت نهاده‏های دامی جاهد، تهران‌ـ‌ایران

6 دانشیار گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران‌ـ‌ایران

7 کارشناس، شرکت صدور احرار شرق، تهران‌ـ‌ایران

چکیده

هدف از پژوهش حاضر، بررسی اثر غلظت‌های متفاوت نانوذرات روی، بر شاخص‏های کیفی منی پس از فرایند انجمادـ‌یخ‏گشایی و بازده آبستنی در گاو بود. از چهار گاو نر هلشتاین به‌مدت چهار هفته و هر هفته دو نوبت اسپرم‌گیری شد. نمونه‏های منی پس از اضافه‌کردن مقادیر صفر (شاهد)، 6-10، 5-10، 4-10، 3-10، و 2-10 مولار نانوذرات روی به رقیق‏کنندۀ بایوکسل، منجمد و پس از 72 ساعت یخ‌گشایی شدند. میزان تحرک اسپرم با نرم‏افزارCASA  ارزیابی شد. بیشترین و کمترین غلظت‌های روی به‌همراه گروه شاهد برای مطالعۀ میزان تکه‏تکه‌شدن DNA، میزان مالون‌دی‌آلدئید، میزان فعالیت میتوکندریایی، و بازده آبستنی ارزیابی شدند. تفاوت میزان حرکت کل و حرکت پیش‌روندۀ اﺳﭙﺮم و میزان تکه‌تکه‌شدن DNA اسپرم‌ها بین تیمارهای آزمایشی معنی‏دار نبود. با این حال، روی در سطوح 2-10 و  6-10 مولار غلظت مالون دی‏آلدئید منی را در مقایسه با گروه شاهد کاهش و فعالیت میتوکندریایی را افزایش داد. درصد آبستنی با اضافه‌کردن نانوذرات روی تغییری نکرد. نتایج نشان داد که افزودن نانوذرات روی به رقیق‌کنندۀ اسپرم گاو سبب کاهش میزان تولید مالون‌دی‌آلدئید و افزایش فعالیت میتوکندری می‌شود ولی بر بهبود نرخ آبستنی تأثیری ندارد. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of zinc nano-complex on bull semen quality and pregnancy outcome

نویسندگان [English]

  • Parisa Yazdanshenas 1
  • Raana Jahanbin 1
  • Abdoullah Mohammadi Sangcheshmeh 2
  • Mehdi Aminafshar 3
  • Hossein Vaseghi Dodaran 4
  • Hamid Varnaseri 5
  • Mohammad Chamani 6
  • Mohammad Hassan Nazaran 7
1 M.Sc., Department of Animal Science and Technology, Faculty of Agriculture and Natural Resources, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran
3 Assistant Professor, Department of Animal Science and Technology, Faculty of Agriculture and Natural Resources, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
4 Ph.D. Student of Animal Physiology, Faculty of Agriculture, University of Tabraiz, Iran
5 Ph.D., Nahadehaye Dami Jahed Company, Tehran, Iran
6 Associate Professor, Department of Animal Science and Technology, Faculty of Agriculture and Natural Resources, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
7 B.Sc., Sodour Ahrar Shargh Company, Tehran, Iran
چکیده [English]

This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. Semen samples diluted with Bioxcell extender containing zero (Control), 10-6, 10-5, 10-4, 10-3 and 10-2 M of zinc nano-complex. The samples were then freezed-thawed after 72 hours. Motility of sperm was analyzed using computer assisted sperm analysis. The highest and lowest concentrations of zinc nano-complex along with control group were analyzed for the DNA fragmentation, malondialdehyde (MDA) concentration, mitochondrial activity, and pregnancy outcomes. The proportion of the total and progressive motile spermatozoa and the percentage of spermatozoa with fragmented DNA were not different among the groups. However, the groups containing 10-6 and 10-2 M zinc nano-complex had a lower level of MDA concentration and higher activity of mitochondria. Moreover, the pregnancy outcome was not affected by the treatment with zinc nano-complex. In conclusion, our data revealed that supplementation of bull semen Bioxcell extender with zinc nano-complex can decrease the level of MDA and improve mitochondrial activity, although such an improvement was not reflected in results of fertility rates.  

کلیدواژه‌ها [English]

  • bull spermatozoa
  • DNA fragmentation
  • mitochondrial-activity
  • pregnancy
  • zinc nano-complex
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