اثر اضافه کردن نانوذرات روی بر شاخص های کیفیت اسپرم گاو بعد از فرآیند انجماد - یخ گشایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، گروه علوم دامی، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

2 استادیار، گروه علوم دامی، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

3 استادیار، گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران، پاکدشت، ایران

4 استادیار، مرکز نهاده‌های دامی جاهد، تهران، ایران

5 دانشیار، گروه علوم دامی، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

6 شرکت صدور احرار شرق، تهران، ایران

چکیده

این تحقیق به‌منظور بررسی اثر غلظت‌های گوناگون نانوذرات روی بر شاخص‏های کیفی منی گاو پس از فرایند انجمادـ‌یخ‏گشایی انجام شد. از چهار گاو نر هلشتاین به مدت چهار هفته و هر هفته دو نوبت اسپرم‏گیری شد. نمونه‌های منی پس از اضافه‌کردن مقادیر صفر، 6-10، 5-10، 4-10، 3-10، و 2-10 مولار نانوذرات روی به رقیق‏کنندۀ بایوکسل، منجمد و پس از 72 ساعت یخ‌گشایی شدند. میزان تحرک اسپرم با نرم‏افزار کاسا (Computer Assisted Sperm Analysis; CASA) ارزیابی شد. میزان زنده‌مانی، یکپارچگی غشا، مورفولوژی، و میزان فعالیت میتوکندریایی اسپرم‏ها در نمونه‏های اسپرم مربوط به بیشترین و کمترین غلظت‏های نانوذرات روی با همراه گروه شاهد ارزیابی شدند. تفاوت در میزان تحرک اﺳﭙﺮم، میزان زنده‌مانی، و درصد اسپرم با ظاهر غیرطبیعی بین غلظت‌های آزمایشی معنی‏دار نبود. داده‏های حاصل از فلوسایتومتری و آزمون هاس نشان داد که یکپارچگی غشا و فعالیت میتوکندریایی اسپرم در گروه‏های 6-10 و 2-10 مولار نانوروی در مقایسه با گروه شاهد بیشتر بود (05/0P<). نتایج پژوهش حاضر نشان داد که با اضافه‌کردن نانوذرات روی به رقیق‏کنندۀ اسپرم گاو یکپارچگی غشا و فعالیت میتوکندریایی اسپرم بیشتر می‏شود. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of zinc nano-complex on bull semen quality after freeze-thawing process

نویسندگان [English]

  • Raana Jahanbin 1
  • Parisa Yazdanshenas 1
  • Mehdi Amin Afshar 2
  • Abdollah Mohammadi Sangcheshmeh 3
  • Hamid Varnaseri 4
  • Mohammad Chamani 5
  • Mohammad Hasan Nazaran 6
  • Mohammad Reza Bakhtiyarizadeh 3
1 M.Sc. Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Assistant Professor, Departments of Animal Science, Abourayhan Campus, University of Tehran, Pakdasht, Iran
4 Assistant Professor, Nahadehaye Dami Jahed Co. Tehran, Iran
5 Associate Professor,, Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
6 Ehrare Hargh Company, Tehran, Iran
چکیده [English]

The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc Nano-complex and frozen. After thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. The proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. Zinc Nano-complex groups represented a higher plasma membrane functionality than that of control group. Moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc Nano-complex possessed higher mitochondrial activity as compared with the control group. Mitochondrial activity in 10-2  was higher than the 10-6 group. In conclusion, supplementation of zn Nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters.

کلیدواژه‌ها [English]

  • anti-oxidant
  • mitochondrial activity
  • semen quality
  • Sperm
  • zinc nano-particles
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