نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه زابل

2 استادیار، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه زابل

3 استادیار، گروه علوم طیور، دانشکدۀ کشاورزی، دانشگاه تربیت مدرس

چکیده

تأثیر پروتکسین در کاهش تأثیرات منفی تغذیة جیره‏های حاوی آفلاتوکسین  B1 بر عملکرد، پاسخ ایمنی، کیفیت گوشت و فلور میکروبی ایلئوم با استفاده از 320 قطعه بلدرچین ژاپنی هفت‌روزه در قالب طرح کاملاً تصادفی با چهار تیمار (جیرة شاهد (بدون افزودنی)، جیرة حاوی 5/2 میلی‏گرم در کیلوگرم آفلاتوکسین B1، جیرة حاوی 150 میلی‏گرم در کیلوگرم پروتکسین، جیرة حاوی 5/2 میلی‏گرم آفلاتوکسین B1+150 میلی‏گرم در کیلوگرم پروتکسین)، چهار تکرار و 20 پرنده در هر تکرار بررسی شد. مصرف خوراک در پرندگانی که با جیرة حاوی پروبیوتیک تغذیه شدند، بالاتر از پرندگان گروه شاهد بود (05/0P<). افزایش وزن پرندگانی که جیرة حاوی آفلاتوکسین دریافت کردند، کمتر از پرندگان تیمارهای دیگر بود (05/0P<). پاسخ ایمنی هومورال در بلدرچین‏های گروه آفلاتوکسین و پروبیوتیک به‏ترتیب کمتر و بیشتر از گروه شاهد بود (05/0P<). ضخامت پوست 48 ساعت پس از چالش با دی‏نیتروکلروبنزن در پرندگان تغذیه‌شده با جیرة آلوده به آفلاتوکسین کمتر از سایر تیمارها بود (05/0P<). غلظت مالون‏دی‏آلدئید (30 روز بعد از انجماد) در گوشت پرندگانی که با جیرة آلوده به آفلاتوکسین تغذیه شدند، بیشتر از سایر پرندگان بود (05/0P<). جمعیت اشریشیاکلی و باکتری‏های اسیدلاکتیک در پرندگانی که با جیرة حاوی پروتکسین و یا آفلاتوکسین+پروتکسین تغذیه شدند، از دو گروه دیگر به‌ترتیب کمتر و بیشتر بود (05/0P<). براساس نتایج پژوهش حاضر، افزودن پروبیوتیک پروتکسین به جیره‏های آلوده به آفلاتوکسین B1 موجب بهبود پاسخ ایمنی و جمعیت میکروبی روده در بلدرچین ژاپنی می‏شود.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Protexin probiotics supplementation to aflatoxin contaminated diet on performance of Japanese quail

نویسندگان [English]

  • Majid Aftabi 1
  • Farzad Bagherzadeh Kasmani 2
  • Ghasem Jalilvan 2
  • Mehran Mehri 2
  • Mohammad Amir Karimi Torshizi 3

1 M.Sc. Student, Department of Animal Science, Faculty of Agriculture, University of Zabol, Iran

2 Assistant Professors, Department of Animal Science, Faculty of Agriculture, University of Zabol, Iran

3 Assistant Professor, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Iran

چکیده [English]

Effect of Protexin to diminish negative consequences of feeding aflatoxin B1 (AFB1) contaminated diet on performance, immune response, meat quality and ileal microbial flora were examined using 320 seven-day-old Japanese quails fed four treatments [control diet (without additives), diet containing 2.5 mg AFB1/kg, diet containing 150 mg Protexin/kg, diet containing 2.5 mg AFB1/kg+150 mg Protexin/kg] with four replicates and 20 birds each in a completely randomized design. Feed intake of birds fed probiotic diet was higher than those birds in control group (P<0.05). Weight gain in birds fed aflatoxin contaminated diet was lower than birds in other treatments (P<0.05). Humoral immunity response in quails related to aflatoxin and probiotic groups was lower and higher than control group, respectively (P<0.05). Skin thickness increase  after 48h challenging with dinitrochlorobenzene (DNCB) in birds fed aflatoxin contaminated diet was lower than the other groups (P<0.05). Concentration of malondialdehyde (after freezing meat for 30 days) in the meat of aflatoxin fed birds was higher than the other birds (P<0.05). Population of E. coli and lactic acid bacteria in birds fed protexin or aflatoxin+protexin was lower and higher than the two other groups, respectively (P<0.05). Based on the result of this research, dietary inclusion of protexin probiotics into aflatoxin contaminated diet could improve immunity response and intestinal microbial population in Japanese quails.

کلیدواژه‌ها [English]

  • Aflatoxicosis
  • Immunity
  • Japanese quail
  • Malondialdehyde
  • Probiotics
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