بررسی چندشکلی ناحیه اگزون سوم ژن FABP4 در دو نژاد سیستانی و دشتیاری و ارتباط آن با صفات رشد در گاو‌های نژاد سیستانی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه زابل

2 استادیار، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه زابل

چکیده

چند‏شکلی ناحیۀ اگزون سوم جایگاه ژن FABP4 و ارتباط الگو‏های ژنوتیپی شناسایی‌شده با صفات مرتبط با رشد با استفاده از 45 رأس گاو شامل نژاد سیستانی (30 رأس) و دشتیاری (15 رأس) بررسی شد. DNA از خون کامل استخراج و کیفیت آنها به‌کمک الکتروفورز ژل آگارز یک درصد بررسی شد. پس از تکثیر زنجیره‏ای پلی‏مرازی (PCR) و هضم آنزیمی آنها با NlaIII و الکتروفورز روی ژل آگارز 8/2 درصد، الگو‏های ژنوتیپی دام‏ها براساس اندازه و تعداد باند‏ها تعیین شد. فراوانی نسبی الگو‏های باندی شناسایی‌شده شامل ژنوتیپ‏هایAA ، AB، و BB در دو نژاد سیستانی و دشتیاری به‌ترتیب 67، 30، و 3 و نیز 73، 27، و صفر درصد بودند. فراوانی آلل‏های A و  Bدر جایگاه مطالعه‌شده در جمعیت سیستانی و دشتیاری به‌ترتیب 82، 18، 5/86، و 5/13 درصد محاسبه شد. شاخص‏‌های هتروزایگوسیتی شامل شاخص شانون (I)، شاخص نئی، هتروزایگوسیتی مشاهده شد و هتروزایگوسیتی مورد انتظار در جمعیت سیستانی و دشتیاری به‌ترتیب 48، 30، 30 و30 و 39، 11، 27 و 24 درصد برآورد شد. الگو‏های ژنوتیپی در جایگاه ژن FABP4 در جمعیت گاو سیستانی همبستگی معنی‌داری با وزن‌های شش، نه‌، و دوازده ماهگی نشان داد. بنابراین جایگاه مذکور می‏تواند به‌عنوان ژن کاندیدا در توصیف تنوع صفات مرتبط با رشد بعد از سن ازشیرگیری گوساله‏ها در برنامه‏های اصلاح نژادی استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

FABP4 gene polymorphism in Sistani and Dashtiari cattle and its association with growth traits in Sistani cattle

نویسندگان [English]

  • Samane Abuli 1
  • Gholam Reza Dashab 2
  • Mohammad Rokouei 2
  • Mehdi Vafaei valeh 2
1 M.Sc., Department of Animal Science, Faculty of Agriculture, University of Zabol, Iran
2 Assistant Professors, Department of Animal Science, Faculty of Agriculture, University of Zabol, Iran
چکیده [English]

The polymorphism in exon three of FABP4 gene and its association with growth traits of 45 Sistani (n=30) and Dashtiari (n=15) cattle were investigated. DNA extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. Animal genotypes were determined based on polymerase chain reaction (PCR) products and their band size electrophoresed on agarose 2.8 percent resulted from enzyme digestion by NlaIII. The pattern of bands showed three genotypes including AA, AB and BB in two Sistani and Dashtiari breeds with frequency of 67, 30 and three percent and 73, 27 and zero percent, respectively. The frequency of A and B alleles in exon three of FABP4 in Sistani and Dashtiari breeds were 82 and 18 percent and 86.5 and 13.5 percent, respectively. Heterozygosity indices including Shannon index (I), Nei’s index, observed and expected heterozygosity in Sistani and Dashtiari population were 48, 30, 30 and 30 percent and 39, 11, 27 and 24 percent, respectively. The association between genotypes and growth-related traits were significant for body weights in six, nine and 12 months of age. Therefore, this locus can be considered as a candidate gene in breeding programs for describing the variation of growth traits after weaning age in calves.

کلیدواژه‌ها [English]

  • FABP4 gene
  • heterozygosity
  • native breeds
  • Polymorphism Information Content
  • Sistani breed
1 . یزدان‏پناه ا، خدرزاده ص و محمدی‌کفتر‌کاری ع (1389) تعیین میزان هتروزایگوسیتی در گاو نجدی استان خوزستان با استفاده از تکنیک PBR. چهارمین کنگرة علوم دامی ایران. کرج. صص. 2875-2878.
 
2 . Adoligbe C, Linsen Zan C, Farougou S, Hongbao Wang J and Ujjan A (2012) Bovine GDF10 gene polymorphism analysis and its association with body measurement traits in Chinese indigenous cattle. Molecular Biology Reproduction. 39: 4067-4075.
3 . Anton I, Kovacs K, Hollo G, Farkas V, Lehel L, Hajda Z and Zsolnai A )2010( Effect of leptin, DGAT1 and TG gene polymorphisms on the intramuscular fat of Angus cattle in Hungary. Livestock Science. 11: 243-249.
4 . Ayres DR, Souza FRP, Mercadante MEZ, Fonseca LFS, TonhatiH, Cyrillo JNSG, Bonilha SFM and Albuquerque LG )2010) Evaluation of TFAM and FABP4 gene polymorphisms in three lines of Nellore cattle selected for growth. Genetics Molecular Resource. 9: 2050-2059.
5 . Barendse W, Bunch RJ, Thomas MB and Harrison BE) 2009( A splice site single nucleotide polymorphisms of the fatty acid binding protein 4 gene appears to be associated with intramuscular fat deposition in longissius muscle in Australian cattle. Animal Genetics. 40: 770-773.
6 . Botstein D, White RL, Skolnick M and Davis RW (1980) Construction of a genetic-linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetic. 32: 314-331.
7 . Cabré A, Lázaro I, Girona J, Manzanares JM, Marimon F and Plana N (2008( Plasma fatty acid binding protein 4 is associated with atherogenic dyslipidemia in diabetes. Lipid Resource. 49(8): 1746-51.
8 . Chen CH, Lin EC, Cheng WTK, Sun HS, Mersmann HJ and Ding ST )2006( Abundantly expressed genes in pig adipose tissue: An expressed sequence tag approach. Animal Science.84: 2673-2683.
9 . Chmurzynska A)2006( The multi-gene family of fatty acid-binding proteins (FABPs): function, structure and polymorphism. Applied Genetics. 47: 39-48.
10 . Cho S, Park TS, Yoon DH, Cheong HS, Namgoong S, Park BL, Lee HW, Han CS, Kim EM, Cheong IC, Kim H and Shin HD (2007) Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle. BMB reports. Pp. 29-34.
11 . Furuhashi M and Hotamisligil GS )2008( Fatty acid binding proteins: role in metabolic diseases and potential as drug targets. National Revolution Drug Discovery.7: 489-503.
12 . Haluzík MM, Anderlová K, Dolezalová R, Adamíková A, Haluzíková D and Housová J (2009( Serum adipocyte fatty acid binding protein levels in patients with type 2 diabetes mellitus and obesity: the influence of fenofibrate treatment. Physiology Resource.58: 93-9.
13 . Havel PJ )2002( Control of energy homeostasis and insulin action by adipocyte hormones: Leptin, acylation stimulating protein, and adiponectin. Current Opinion in Lipidology. 13: 51-59.
14 . Jiang Z, Kunej T and Michal JJ (2005(  Biochemistry Biophysics Resource Community. 334: 516-523.
15 . Kulig H, Kowalewska-Łuczak I, Kmieć M and Wojdak-Maksymiec K (2010) ANXA9, SLC27A3, FABP3 and FABP4 single nucleotidepolymorphisms in relation to milk production traits in Jersey cows. Czech Journal of Animal Science. 55: 463-467.
16 . Lee SH, Park EW, Cho YM, Kim KH, Oh YK, Lee JH, Lee CS, Oh SJ and Yoon DH (2006) Lipogenesis gene expression profiling on the early and late fattening stage of Hanwoo longissimus dorsi. Animal Science and Technology (Kor). 48: 913-20.
17 . Lee SH, Van der Werf JHJ, Lee SH, Park EW, Oh SJ, Gibson JP and Thompson JM (2010) Genetic polymorphisms of the bovine Fatty acid binding protein 4 gene are significantly associated with marbling and carcass weight in Hanwoo (Korean Cattle). Animal Genetics. 41: 442- 444.
18 . Michal JJ, Zhang ZW, Gaskins CT and Jiang Z)2006( The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyu 9 Limousin F2 crosses. Animal Genetics. 37: 400-402.
19 . Pannier L, Mullen AM, Hamill RM, Stapleton PC and Sweeney T) 2010( Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle. Meat Science. 85: 515-518.
21 . Shin SC, Heo JP and Chung FR)2012( Genetic variants of the FABP4 gene are associated with marbling scores and meat quality grades in Hanwoo (Korean cattle). Molecular Biology Reports. 39: 5323-5330.
22 . Souza FRP, Mercadante MEZ, Fonseca LFS, Ferreira LMS, Regatieri IC, Ayres DR, Tonhati H, Silva SL, Razook AG and Albuquerque LG)2010( Assessment of DGAT1and LEP gene polymorphisms in three Nelore (Bos indicus) lines selected for growth and their relationship with growth and carcass traits. Animal Science. 88: 435-441.
23 . Thaller G, Kuhn C, Winter A, Ewald G, Bellmann O, Wegner J, Zuhlke H and Fries R)2003( DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Animal Genetics. 34: 354-357.
24 . Tso AW, Xu A, Sham PC, Wat NM, Wang Y and Fong CHY)2007( Serum adipocyte fatty acid binding protein as a new biomarker predicting the development of type 2 diabetes: a 10-year prospective study in a Chinese cohort. Diabetes Care. 30: 2667-2672.
25 . Tuncman G, Erbay E, Hom X, DeVivo I, Campos H and Rimm EB (2006( A genetic variant at the fatty acid-binding protein aP2 locus reduces the risk for hypertri- glycer-idemia, type 2 diabetes, and cardiovascular disease. Proceedings of the National Academy of Sciences. 103: 6970-6975.
26 . White SN, Casas E, Allan MF, Keele JW, Snelling WM, Wheeler TL, Shackelford SD, Koohmaraie M and Smith TPL)2007( Evaluation in beef cattle of six deoxy-ribo-nucleic-acid markers developed for dairy traits reveals an osteopontin polymorphism associated with post-weaning growth. Animal Science.85: 1-10.
27 . Wimmers K, Murani E, Schellander K and Ponsuksili S (2005) Combining QTL and expression-analysis: identification of functional positional candidate genes for meat quality and carcass traits. Archiv Tierzucht. 48: 23-31.
28 . Xu A, Wang Y, Xu JY, Stejskal D, Tam S and Zhang J (2006( Adipocyte fatty acid binding protein is a plasma biomarker closely associated with obesity and metabolic syndrome. Clinical Chemistry. 52: 405-413.
29 . Xu QL, Tang GW,  Zhang QL, Huang YK, Liu YX, Quan K, Zhu KY and Zhang CX )2011( The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds. Czech Journal Animal Science. 56: 1-6.
30 . Yeh FC, Yang RC and Boyle T (1999) POPGENE version 1.31 Microsoft window-based freeware for population genetic analysis. University of Alberta and Centre for International Forestry Researchin water Catchments Systems. 416-432 pp: 45-54.