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بررسی چندشکلی ناحیه اگزون سوم ژن FABP4 در دو نژاد سیستانی و دشتیاری و ارتباط آن با صفات رشد در گاو‌های نژاد سیستانی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه زابل

2 استادیار، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه زابل

چکیده

چند‏شکلی ناحیۀ اگزون سوم جایگاه ژن FABP4 و ارتباط الگو‏های ژنوتیپی شناسایی‌شده با صفات مرتبط با رشد با استفاده از 45 رأس گاو شامل نژاد سیستانی (30 رأس) و دشتیاری (15 رأس) بررسی شد. DNA از خون کامل استخراج و کیفیت آنها به‌کمک الکتروفورز ژل آگارز یک درصد بررسی شد. پس از تکثیر زنجیره‏ای پلی‏مرازی (PCR) و هضم آنزیمی آنها با NlaIII و الکتروفورز روی ژل آگارز 8/2 درصد، الگو‏های ژنوتیپی دام‏ها براساس اندازه و تعداد باند‏ها تعیین شد. فراوانی نسبی الگو‏های باندی شناسایی‌شده شامل ژنوتیپ‏هایAA ، AB، و BB در دو نژاد سیستانی و دشتیاری به‌ترتیب 67، 30، و 3 و نیز 73، 27، و صفر درصد بودند. فراوانی آلل‏های A و  Bدر جایگاه مطالعه‌شده در جمعیت سیستانی و دشتیاری به‌ترتیب 82، 18، 5/86، و 5/13 درصد محاسبه شد. شاخص‏‌های هتروزایگوسیتی شامل شاخص شانون (I)، شاخص نئی، هتروزایگوسیتی مشاهده شد و هتروزایگوسیتی مورد انتظار در جمعیت سیستانی و دشتیاری به‌ترتیب 48، 30، 30 و30 و 39، 11، 27 و 24 درصد برآورد شد. الگو‏های ژنوتیپی در جایگاه ژن FABP4 در جمعیت گاو سیستانی همبستگی معنی‌داری با وزن‌های شش، نه‌، و دوازده ماهگی نشان داد. بنابراین جایگاه مذکور می‏تواند به‌عنوان ژن کاندیدا در توصیف تنوع صفات مرتبط با رشد بعد از سن ازشیرگیری گوساله‏ها در برنامه‏های اصلاح نژادی استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

FABP4 gene polymorphism in Sistani and Dashtiari cattle and its association with growth traits in Sistani cattle

نویسندگان [English]

  • Samane Abuli 1
  • Gholam Reza Dashab 2
  • Mohammad Rokouei 2
  • Mehdi Vafaei valeh 2

1 M.Sc., Department of Animal Science, Faculty of Agriculture, University of Zabol, Iran

2 Assistant Professors, Department of Animal Science, Faculty of Agriculture, University of Zabol, Iran

چکیده [English]

The polymorphism in exon three of FABP4 gene and its association with growth traits of 45 Sistani (n=30) and Dashtiari (n=15) cattle were investigated. DNA extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. Animal genotypes were determined based on polymerase chain reaction (PCR) products and their band size electrophoresed on agarose 2.8 percent resulted from enzyme digestion by NlaIII. The pattern of bands showed three genotypes including AA, AB and BB in two Sistani and Dashtiari breeds with frequency of 67, 30 and three percent and 73, 27 and zero percent, respectively. The frequency of A and B alleles in exon three of FABP4 in Sistani and Dashtiari breeds were 82 and 18 percent and 86.5 and 13.5 percent, respectively. Heterozygosity indices including Shannon index (I), Nei’s index, observed and expected heterozygosity in Sistani and Dashtiari population were 48, 30, 30 and 30 percent and 39, 11, 27 and 24 percent, respectively. The association between genotypes and growth-related traits were significant for body weights in six, nine and 12 months of age. Therefore, this locus can be considered as a candidate gene in breeding programs for describing the variation of growth traits after weaning age in calves.

کلیدواژه‌ها [English]

  • FABP4 gene
  • heterozygosity
  • native breeds
  • Polymorphism Information Content
  • Sistani breed
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تولیدات دامی
دوره 17، شماره 1 - شماره پیاپی 1
بهار و تابستان 1394
فروردین 1394
صفحه 107-117
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هم رسانی
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