نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار گروه علوم دامی، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، ایران

2 استاد گروه تغذیۀ دام و طیور، دانشکدۀ علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

3 استادیار پژوهشگاه علوم و فنون هسته‏ای، پژوهشکدۀ کشاورزی هسته ‏ای کرج، ایران

4 استادیار بخش تغذیه و فیزیولوژی دام و طیور، مؤسسۀ تحقیقات علوم دامی کشور، کرج، ایران

چکیده

این پژوهش به‏منظور بررسی تأثیر دوزهای 25، 50 و 75 کیلوگری پرتوهای یون‏ساز گاما و الکترون، بر ناپدیدشدن شکمبه‏ای اسیدهای آمینه و زیرواحدهای پروتئین کنجالۀ کانولا انجام گرفت. آزمایش تجزیه‏پذیری با تکنیک کیسه‏های نایلونی اجرا شد. بدین ‏منظور از سه رأس گاو نر تالشی مجهز به فیستولای شکمبه‏ای استفاده شد. روند ناپدیدشدن شکمبه‏ای زیرواحدهای پروتئین کنجالۀ کانولا، با تکنیک الکتروفورز ژل پلی‏آکریلامید در حضور سدیم دودسیل‌سولفات تعیین شد. پرتوتابی موجب کاهش تجزیه‏پذیری شکمبه‏‏‏ای اسیدهای آمینه شد (05/0P<). به‏جز در زمینۀ اسیدهای آمینۀ سرین، تیروزین، و گلوتامات، تأثیر پرتو گاما در کاهش تجزیه‏پذیری اسیدهای آمینه بیشتر از پرتو الکترون بود. تجزیۀ الکتروفورزی وجود پروتئین کروسیفرین با چهار زیرواحد را در کنجالۀ کانولا نشان داد. در کنجالۀ کانولای پرتوتابی‌نشده، هر چهار زیرواحد پروتئین کروسیفرین پس از هشت ساعت انکوباسیون شکمبه‏ای تجزیه شدند. دوزهای 25، 50 و 75 کیلوگری پرتو الکترون و پرتو گاما، باعث حفظ زیرواحدهای پروتئین کروسیفرین به‏ترتیب تا زمان 16، 24 و 48 ساعت شدند. نتایج تحقیق حاضر نشان داد که عمل‏آوری با پرتوهای یون‏ساز گاما و الکترون باعث کاهش تجزیۀ شکمبه‏ای زیرواحدهای پروتئین کروسیفرین و اسیدهای آمینۀ کنجالۀ کانولا می‏شود. توانایی پرتو گاما در کاهش تجزیۀ شکمبه‏ای پروتئین‏های کنجالۀ کانولابیشتر از پرتو الکترون است.

کلیدواژه‌ها

عنوان مقاله [English]

Ruminal disappearance of protein and amino acids of untreated and treated canola meal with gamma ray and electron beam

نویسندگان [English]

  • Farzad Ghanbari 1
  • Taghi Ghoorchi 2
  • Parvin Shawrang 3
  • Hormoz Mansouri 4
  • Nour Mohammad Torbatinejad 2

1 Assistant Professor, Department of Animal Science, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Iran

2 Professor, Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Iran

3 Assistant Professor, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran

4 Assistant Professor, Department of Nutrition and Phisiology of Animal and Poultry, Animal Science Research Institute, Karaj, Iran

چکیده [English]

This study was conducted to investigate the effects of doses of 25, 50 and 75 kGy of gamma ray (GR) and electron beam (EB) ionizing radiations on ruminal disappearance of amino acids (AAs) and protein subunits of canola meal (CM). The nylon bag technique was used for degradability trial. Three ruminally fistulated Taleshi bulls were used for this aim. The disappearance trends of protein subunits for protein meal samples were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Irradiation decreased ruminal degradability of AAs (P<0.05). In this case, except for serine, tyrosine and glutamate, the effect of GR in reducing AAs degradability was more than the EB. Electrophoresis analysis identified the presence of cruciferin with four subunits in CM. In unirradiated CM, all four protein subunits of cruciferin were degraded after 8 h of rumen incubation. Doses of 25, 50 and 75 kGy of EB and GR preserved the protein subunits of cruciferin up to 16, 24 and 48 h, respectively. It can be concluded that processing with ionizing radiation of EB and GR decreases ruminal degradation of CM protein subunits of cruciferin and AAs. The effect of GR in reducing ruminal degradation of CM proteins is more than EB.

کلیدواژه‌ها [English]

  • Canola meal
  • cruciferin
  • Degradability
  • electrophoresis
  • ionizing radiations

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